<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>In these studies we attempted to evaluate the lipolytic, proteolytic and cellulolytic activity of bacterial strains isolated from water and the bottom sediments of Turawa Lake. The following bacterial genera prevailed among the isolated strains: <italic>Bacillus, Pseudomonas, Enterobacter, Cellulomonas</italic> and <italic>Cytophaga.</italic> The lipolytic activity was determined using a titrimetric method, the proteolytic activity — using a modified Anson method, and the cellulolytic activity - on the basis of mass decrement of a cellulose disk after 14 days of bacterial culture. The cultures were maintained at 28°C, pH 7.0 with the following substrates: olive oil, albumin and cellulose disk. Among the analysed microorganisms, <italic>Bacillus</italic> and <italic>Pseudomonas</italic> strains showed the highest lipolytic and proteolytic activity. In the cellulolytic assay <italic>Cytophaga</italic> bacteria showed about twofold higher activity than that of <italic>Celulomonas.</italic></p></abstract>

In these studies we attempted to evaluate the lipolytic, proteolytic and cellulolytic activity of bacterial strains isolated from water and the bottom sediments of Turawa Lake. The following bacterial genera prevailed among the isolated strains: Bacillus, Pseudomonas, Enterobacter, Cellulomonas and Cytophaga. The lipolytic activity was determined using a titrimetric method, the proteolytic activity — using a modified Anson method, and the cellulolytic activity - on the basis of mass decrement of a cellulose disk after 14 days of bacterial culture. The cultures were maintained at 28°C, pH 7.0 with the following substrates: olive oil, albumin and cellulose disk. Among the analysed microorganisms, Bacillus and Pseudomonas strains showed the highest lipolytic and proteolytic activity. In the cellulolytic assay Cytophaga bacteria showed about twofold higher activity than that of Celulomonas.

Evaluation of the enzymatic activity of selected bacterial strains

Biotechnology and Molecular Biology, Opole University, ul. Kominka 4, 45-032 Opole, Poland

Polish Journal of Chemical Technology

{"article-title":"Evaluation of the enzymatic activity of selected bacterial strains"}

In these studies we attempted to evaluate the lipolytic, proteolytic and cellulolytic activity of bacterial strains isolated from water and the bottom...

Evaluation of the enzymatic activity of selected bacterial strains

Online veröffentlicht: 19. Okt. 2007

Seitenbereich: 68 - 72

DOI: https://doi.org/10.2478/v10026-007-0030-y

Schlüsselwörter
bottom sediment, bacteria, lipolytic, proteolytic and cellulolytic activity

This content is open access.

Evaluation of the enzymatic activity of selected bacterial strains

Online veröffentlicht: 19. Okt. 2007

Seitenbereich: 68 - 72

DOI: https://doi.org/10.2478/v10026-007-0030-y

Schlüsselwörterbottom sediment, bacteria, lipolytic, proteolytic and cellulolytic activity

This content is open access.

Schlüsselwörter
bottom sediment, bacteria, lipolytic, proteolytic and cellulolytic activity