<abstract xmlns="http://www.w3.org/1999/xhtml"><p>We evaluated the influence of some morphological changes of the NCI-H125 cell line in surface expression of the epidermal growth factor receptor (EGFR) and their impact on some biological activity assays using this molecule as target. Hematoxylin and eosin (H/E) staining, light microscopy immunocytochemistry, flow cytometric antibody-receptor binding test, cell viability determination and cell cycle analysis were performed. Phenotypic changes and inconsistency in EGFR expression were detected in NCI-H125 cell cultures. A significant reduction in the growth rate, mainly characterized by cell cycle arrest in the G0-G1 phase, was also evidenced. Differential distribution of cell viability in NCI-H125 subpopulations and its relationship with the EGFR surface expression were determined. Nuclear alterations observed in NCI- -H125 were not apoptosis related. A lack of control of cell cultures affects the reliability and reproducibility of biomedical and biotechnological research using EGFR as target. Therefore, rigorous control of the above mentioned parameters in these experiments is recommended.</p></abstract>

We evaluated the influence of some morphological changes of the NCI-H125 cell line in surface expression of the epidermal growth factor receptor (EGFR) and their impact on some biological activity assays using this molecule as target. Hematoxylin and eosin (H/E) staining, light microscopy immunocytochemistry, flow cytometric antibody-receptor binding test, cell viability determination and cell cycle analysis were performed. Phenotypic changes and inconsistency in EGFR expression were detected in NCI-H125 cell cultures. A significant reduction in the growth rate, mainly characterized by cell cycle arrest in the G0-G1 phase, was also evidenced. Differential distribution of cell viability in NCI-H125 subpopulations and its relationship with the EGFR surface expression were determined. Nuclear alterations observed in NCI- -H125 were not apoptosis related. A lack of control of cell cultures affects the reliability and reproducibility of biomedical and biotechnological research using EGFR as target. Therefore, rigorous control of the above mentioned parameters in these experiments is recommended.

Phenotypic heterogeneity in the NCI-H125 cell line affects biological activity using the epidermal growth factor receptor as target / Fenotipska heterogenost u staničnoj liniji NCI-H125 utječe na biološku aktivnost na receptore za epidermalni faktor rasta

Laboratory of Specific Recognition and Biological Activity Assays, Department of Quality Control, Center of Molecular Immunology, Havana, Cuba

Laboratory of Virology, Department of Quality Control, Center of Molecular Immunology, Havana, Cuba

Laboratory of Microbiology, Department of Quality Control, Center of Molecular Immunology, Havana, Cuba

Acta Pharmaceutica

We evaluated the influence of some morphological changes of the NCI-H125 cell line in surface expression of the epidermal growth factor receptor (EGFR) and...

Phenotypic heterogeneity in the NCI-H125 cell line affects biological activity using the epidermal growth factor receptor as target / Fenotipska heterogenost u staničnoj liniji NCI-H125 utječe na biološku aktivnost na receptore za epidermalni faktor rasta

Online veröffentlicht: 15. Jan. 2013

Seitenbereich: 581 - 591

DOI: https://doi.org/10.2478/v10007-012-0038-6

Schlüsselwörter
cell viability, morphological changes, biological activity, epidermal growth factor receptor, nimotuzumab

This content is open access.

Phenotypic heterogeneity in the NCI-H125 cell line affects biological activity using the epidermal growth factor receptor as target / Fenotipska heterogenost u staničnoj liniji NCI-H125 utječe na biološku aktivnost na receptore za epidermalni faktor rasta

Online veröffentlicht: 15. Jan. 2013

Seitenbereich: 581 - 591

DOI: https://doi.org/10.2478/v10007-012-0038-6

Schlüsselwörtercell viability, morphological changes, biological activity, epidermal growth factor receptor, nimotuzumab

This content is open access.

Schlüsselwörter
cell viability, morphological changes, biological activity, epidermal growth factor receptor, nimotuzumab