<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">In this study, five compounds, lupeol (<bold>1</bold>), epilupeol (<bold>2</bold>), β-sitosterol (<bold>3</bold>), stigmasterol (<bold>4</bold>) and <italic>p</italic>-methoxybenzaldehyde (<bold>5</bold>) were isolated from the petroleum ether and dichloromethane fractions of a methanolic extract of the stem bark of <italic>Delonix regia</italic>. Antimicrobial screening of the different extracts (15 μg mm-2) was conducted by the disc diffusion method. The zones of inhibition demonstrated by the petroleum ether, carbon tetrachloride and dichloromethane fractions ranged from 9-14 mm, 11-13 mm and 9-20 mm, respectively, compared to kanamycin standard with the zone of inhibition of 20-25 mm. In brine shrimp lethality bioassay, the carbon tetrachloride soluble materials demonstrated the highest toxicity with <italic>LC</italic>50 of 0.83 μg mL-1, while petroleum ether and dichloromethane soluble partitionates of the methanolic extract revealed <italic>LC</italic>50 of 14.94 and 3.29 μg mL-1, respectively, in comparison with standard vincristine sulphate with <italic>LC</italic>50 of 0.812 μg mL-1. This is the first report on compounds separation from <italic>D. regia</italic>, their antimicrobial activity and cytotoxicity.</abstract>

In this study, five compounds, lupeol (1), epilupeol (2), β-sitosterol (3), stigmasterol (4) and p-methoxybenzaldehyde (5) were isolated from the petroleum ether and dichloromethane fractions of a methanolic extract of the stem bark of Delonix regia. Antimicrobial screening of the different extracts (15 μg mm-2) was conducted by the disc diffusion method. The zones of inhibition demonstrated by the petroleum ether, carbon tetrachloride and dichloromethane fractions ranged from 9-14 mm, 11-13 mm and 9-20 mm, respectively, compared to kanamycin standard with the zone of inhibition of 20-25 mm. In brine shrimp lethality bioassay, the carbon tetrachloride soluble materials demonstrated the highest toxicity with LC50 of 0.83 μg mL-1, while petroleum ether and dichloromethane soluble partitionates of the methanolic extract revealed LC50 of 14.94 and 3.29 μg mL-1, respectively, in comparison with standard vincristine sulphate with LC50 of 0.812 μg mL-1. This is the first report on compounds separation from D. regia, their antimicrobial activity and cytotoxicity.

Chemical and biological investigations of <italic>Delonix regia</italic> (Bojer ex Hook.) Raf.

Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000 Bangladesh

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh

Department of Pharmacy, The University of Asia Pacific, Dhaka-1209, Bangladesh

Department of Skin and Veneral Diseases, Bangabandhu Sheikh Mujib Medical University, Shahbag Dhaka-1000, Bangladesh

Acta Pharmaceutica

Chemical and biological investigations of  (Bojer ex Hook.) Raf.

In this study, five compounds, lupeol (1), epilupeol (2), β-sitosterol (3), stigmasterol (4) and p-methoxybenzaldehyde (5) were isolated from the petroleum...

Chemical and biological investigations of Delonix regia (Bojer ex Hook.) Raf.

Online veröffentlicht: 06. Dez. 2010

Seitenbereich: 207 - 215

DOI: https://doi.org/10.2478/v10007-010-0018-7

Schlüsselwörter
metanolni ekstrakt, antimikrobno djelovanje, smrtnost morskih kozica

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