<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The reversed-phase preparative high performance liquid chromatographic purification of the methanol extract of the fruits of <italic>Ribes biebersteinii</italic> Berl. (<italic>Grossulariaceae</italic>) afforded five cyanidin glycosides, 3-<italic>O</italic>-sambubiosyl-5-<italic>O</italic>-glucosyl cyanidin (<bold>1</bold>), cyanidin 3-<italic>O</italic>-sambubioside (<bold>2</bold>), cyanidin 3-<italic>O</italic>-glucoside (<bold>3</bold>), cyanidin 3-<italic>O</italic>-(2G-xylosyl)-rutinoside (<bold>4</bold>) and cyanidin 3-<italic>O</italic>-rutinoside (<bold>5</bold>). They showed considerable free-radical-scavenging properties in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay with the <italic>R</italic>C50 values of 9.29 × 10-6, 9.33 × 10-6, 8.31 × 10-6, 8.96 × 10-6 and 9.55 × 10-6 mol L-1, respectively. The structures of these compounds were elucidated by various chemical hydrolyses and spectroscopic means. The total anthocyanin content was 1.9 g per 100 g dried fruits on cyanidin 3-glucoside basis.</abstract>

The reversed-phase preparative high performance liquid chromatographic purification of the methanol extract of the fruits of Ribes biebersteinii Berl. (Grossulariaceae) afforded five cyanidin glycosides, 3-O-sambubiosyl-5-O-glucosyl cyanidin (1), cyanidin 3-O-sambubioside (2), cyanidin 3-O-glucoside (3), cyanidin 3-O-(2G-xylosyl)-rutinoside (4) and cyanidin 3-O-rutinoside (5). They showed considerable free-radical-scavenging properties in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay with the RC50 values of 9.29 × 10-6, 9.33 × 10-6, 8.31 × 10-6, 8.96 × 10-6 and 9.55 × 10-6 mol L-1, respectively. The structures of these compounds were elucidated by various chemical hydrolyses and spectroscopic means. The total anthocyanin content was 1.9 g per 100 g dried fruits on cyanidin 3-glucoside basis.

Isolation and free-radical-scavenging properties of cyanidin 3-<italic>O</italic>-glycosides from the fruits of <italic>Ribes biebersteinii</italic> Berl.

School of Pharmacy, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Drug Discovery and Design Research Division, Department of Pharmacy School of Applied Sciences, University of Wolverhampton, MA Building Wulfruna Street, Wolverhampton WV1 1LY, West Midland, UK

Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, MM Building Molineux Street, Wolverhampton WV1 1SB, West Midland, UK

Acta Pharmaceutica

Isolation and free-radical-scavenging properties of cyanidin 3--glycosides from the fruits of  Berl.

The reversed-phase preparative high performance liquid chromatographic purification of the methanol extract of the fruits of Ribes biebersteinii Berl....

Isolation and free-radical-scavenging properties of cyanidin 3-O-glycosides from the fruits of Ribes biebersteinii Berl.

Online veröffentlicht: 13. März 2010

Seitenbereich: 1 - 11

DOI: https://doi.org/10.2478/v10007-010-0007-x

Schlüsselwörter
Berl. (), 2,2-difenil-1-pikrilhidrazil (DPPH), cijanidin glikozid, hvatač slobodnih radikala

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