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A preliminary study through lymphocyte immunophenotyping of the effects of different forms of phosphorus deficiency on dairy cattle blood haematological parameters and immune status

, ,  und   
13. Juni 2025

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COVER HERUNTERLADEN

Fig. 1.

Gating strategy for immunophenotyping of bovine peripheral blood lymphocytes. A – single-cell gating; B – lymphocyte gating; C–F – fluorescence cytograms of positive cells
Gating strategy for immunophenotyping of bovine peripheral blood lymphocytes. A – single-cell gating; B – lymphocyte gating; C–F – fluorescence cytograms of positive cells

Morphology of blood samples of Friesian dairy cows with atypical or clinical hypophosphataemia

Parameters Scope of standards (17) Group I (control) Group II Group III Group IV
Pi (mmol/L) 1.5–2.9 1.53 ± 0.10A,a 1.12 ± 0.16A,b 0.37 ± 0.05B,c 0.33 ± 0.15B,c
WBC (×109/L) 6.25–9.5 7.55 ± 1.20A,a 5.63 ± 1.03B,b 4.35 ± 1.45B,c 3.50 ± 1.64C,d
GP (%) 0–2 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0
GS (%) 23–37 29.0 ± 7.07A,a 23.0 ± 9.38A,b 37.75 ± 11.09B,c 54.25 ± 7.23C,d
L (%) 53–67 60.0 ± 4.24A,b 68.75 ± 19.26a 54.0 ± 15.51b 41.75 ± 5.19c
TCD4+ (%) 24.64 ± 2.04a 29.38 ± 3.48b 29.44 ± 5.33b 32.51 ± 4.27b
TCD8+ (%) 12.79 ± 0.57a 7.91 ± 0.71b 10.84 ± 2.66A,b 11.61 ± 4.27A,b
TCD4/TCD8 1.94 ± 0.23 3.72 ± 0.45 2.77 ± 0.34 2.89 ± 0.57
MHC II (%) 39.29 ± 2.5a 46.22 ± 3.83A,b 50.64 ± 11.8b 64.20 ± 5.33c
BCD21+ (%) 30.56 ± 5.63 34.10 ± 3.83 34.38 ± 5.07 37.17 ± 9.75
RBC (×1012/L) 5.0–7.0 7.03 ± 1.17a 6.04 ± 0.59a 4.95 ± 0.67b 4.91 ± 0.77b
HGB (g/L) 105–140 101.5 ± 9.19A,a 90.25 ± 10.72A,a 63.75 ± 22.74bi' 73.75 ± 11.41B,b
HCT (L/L) 0.3–0.4 0.33 ± 0.04a 0.28 ± 0.04A,b 0.23 ± 0.03b 0.23 ± 0.04b
MCV (fL) 40–60 46.5 ± 2.12 47.25 ± 2.22 45.75 ± 2.06 47.25 ± 0.96
MCH (fmol/L) 0.9–1.5 0.97 ± 0.03 0.89 ± 0.03 0.90 ± 0.03 0.93 ± 0.02
MCHC (mmol/L) 16–21 19.44 ± 0.48 19.58 ± 0.27 19.47 ± 0.30 19.70 ± 0.48
PLT (×109/L) 200–800 330.5 ± 17.78 647 ± 269.16 469 ± 114.07 388.5 ± 121.33
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
4 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Biologie, Molekularbiologie, Mikrobiologie und Virologie, Biologie, andere, Medizin, Veterinärmedizin