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Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by Staphylococcus aureus

Wenjia Wang

Wenjia Wang

  • College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
  • College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyZhengzhou, PR China
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Wang, Wenjia
, 
Rongling Li

Rongling Li

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Li, Rongling
, 
Tingzhu Ye

Tingzhu Ye

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Ye, Tingzhu
, 
Xinxin Zhang

Xinxin Zhang

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Zhang, Xinxin
, 
Chao Chen

Chao Chen

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Chen, Chao
, 
Ai-xin Liang

Ai-xin Liang

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Liang, Ai-xin
 und 
Li-guo Yang

Li-guo Yang

College of Animal Science and Technology of Huazhong Agricultural UniversityWuhan, PR China
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Yang, Li-guo
  
05. Mai 2022
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Online veröffentlicht: 05. Mai 2022
Seitenbereich: 179 - 187
Eingereicht: 23. Nov. 2021
Akzeptiert: 21. Apr. 2022
DOI: https://doi.org/10.2478/jvetres-2022-0022
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© 2022 W. Wang et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Clinical mastitis (CM) is one of the most common diseases of dairy cows globally, has a complex aetiology and recurs easily. Staphylococcus aureus is a frequently isolated pathogen responsible for bovine mastitis and remains difficult to eradicate.

Material and Methods

To characterise the transcriptional profiles of dairy cows infected by S. aureus, we performed an RNA-seq analysis of peripheral blood leukocytes in lactating Chinese Holstein dairy cows with CM and did the same with healthy cows’ samples as controls.

Results

A total of 4,286 genes were detected in the CM cases infected with S. aureus which were differentially expressed compared to the controls, 3,085 of which were upregulated, the remainder being downregulated. Notably, we observed that some differentially expressed genes (DEGs) had strong protein–protein interaction. Of these, six downregulated DEGs (AKR1C4, PTGS2, HNMT, EPHX2, CMBL, and IDH1) were involved in the metabolic pathway, while eight upregulated DEGs (VWF, GP9, MYLK, GP6, F2RL3, ITGB3, GP5, and PRKG1) were associated with the platelet activation pathway.

Conclusion

The transcriptome dataset of CM cases would be a valuable resource for clinical guidance on anti-inflammatory medication and for deeper understanding of the biological processes of CM response to S. aureus infection, and it would enable us to identify specific genes for diagnostic markers and possibly for targeted therapy.
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