<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2022-0022_s_005">
<h3>Introduction</h3>
<p>Clinical mastitis (CM) is one of the most common diseases of dairy cows globally, has a complex aetiology and recurs easily. <italic>Staphylococcus aureus</italic> is a frequently isolated pathogen responsible for bovine mastitis and remains difficult to eradicate.</p>

</sec>
<sec id="j_jvetres-2022-0022_s_006">
<h3>Material and Methods</h3>
<p>To characterise the transcriptional profiles of dairy cows infected by <italic>S. aureus</italic>, we performed an RNA-seq analysis of peripheral blood leukocytes in lactating Chinese Holstein dairy cows with CM and did the same with healthy cows’ samples as controls.</p></sec>
<sec id="j_jvetres-2022-0022_s_007">
<h3>Results</h3>
<p>A total of 4,286 genes were detected in the CM cases infected with <italic>S. aureus</italic> which were differentially expressed compared to the controls, 3,085 of which were upregulated, the remainder being downregulated. Notably, we observed that some differentially expressed genes (DEGs) had strong protein–protein interaction. Of these, six downregulated DEGs (<italic>AKR1C4</italic>, <italic>PTGS2</italic>, <italic>HNMT</italic>, <italic>EPHX2</italic>, <italic>CMBL</italic>, and <italic>IDH1</italic>) were involved in the metabolic pathway, while eight upregulated DEGs (<italic>VWF</italic>, <italic>GP9</italic>, <italic>MYLK</italic>, <italic>GP6</italic>, <italic>F2RL3</italic>, <italic>ITGB3</italic>, <italic>GP5</italic>, and <italic>PRKG1</italic>) were associated with the platelet activation pathway.</p></sec>
<sec id="j_jvetres-2022-0022_s_008">
<h3>Conclusion</h3>
<p>The transcriptome dataset of CM cases would be a valuable resource for clinical guidance on anti-inflammatory medication and for deeper understanding of the biological processes of CM response to <italic>S. aureus</italic> infection, and it would enable us to identify specific genes for diagnostic markers and possibly for targeted therapy.</p></sec>
</abstract>

Introduction
Clinical mastitis (CM) is one of the most common diseases of dairy cows globally, has a complex aetiology and recurs easily. Staphylococcus aureus is a frequently isolated pathogen responsible for bovine mastitis and remains difficult to eradicate.



Material and Methods
To characterise the transcriptional profiles of dairy cows infected by S. aureus, we performed an RNA-seq analysis of peripheral blood leukocytes in lactating Chinese Holstein dairy cows with CM and did the same with healthy cows’ samples as controls.

Results
A total of 4,286 genes were detected in the CM cases infected with S. aureus which were differentially expressed compared to the controls, 3,085 of which were upregulated, the remainder being downregulated. Notably, we observed that some differentially expressed genes (DEGs) had strong protein–protein interaction. Of these, six downregulated DEGs (AKR1C4, PTGS2, HNMT, EPHX2, CMBL, and IDH1) were involved in the metabolic pathway, while eight upregulated DEGs (VWF, GP9, MYLK, GP6, F2RL3, ITGB3, GP5, and PRKG1) were associated with the platelet activation pathway.

Conclusion
The transcriptome dataset of CM cases would be a valuable resource for clinical guidance on anti-inflammatory medication and for deeper understanding of the biological processes of CM response to S. aureus infection, and it would enable us to identify specific genes for diagnostic markers and possibly for targeted therapy.

Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by <italic>Staphylococcus aureus</italic>

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by 

Introduction
Clinical mastitis (CM) is one of the most common diseases of dairy cows globally, has a complex aetiology and recurs easily. Staphylococcus aureus...

Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by Staphylococcus aureus

Online veröffentlicht: 05. Mai 2022

Seitenbereich: 179 - 187

Eingereicht: 23. Nov. 2021

Akzeptiert: 21. Apr. 2022

DOI: https://doi.org/10.2478/jvetres-2022-0022

Schlüsselwörter
clinical mastitis, cattle, inflammatory response, RNA-seq

© 2022 W. Wang et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Preliminary study on gene regulation and its pathways in Chinese Holstein cows with clinical mastitis caused by Staphylococcus aureus

Online veröffentlicht: 05. Mai 2022

Seitenbereich: 179 - 187

Eingereicht: 23. Nov. 2021

Akzeptiert: 21. Apr. 2022

DOI: https://doi.org/10.2478/jvetres-2022-0022

Schlüsselwörterclinical mastitis, cattle, inflammatory response, RNA-seq

© 2022 W. Wang et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
clinical mastitis, cattle, inflammatory response, RNA-seq