S. putrefaciens isolates | |||||||||
---|---|---|---|---|---|---|---|---|---|
Biochemical properties | ATCC BAA-1097 | ATCC 8071 | ATCC 8073 | ATCC 700550 | JCM 16212T | ||||
Ornithine decarboxylase | 6 | 1 | 14 | 5 | - | - | − | + | + |
Citrate utilisation | 2 | 0 | 4 | 2 | +w | +w | − | + | + |
Acid from arabinose | 9 | 0 | 3 | 1 | + | + | + | − | + |
Aesculin hydrolysis | 9 | 4 | 23 | 6 | +w | +w | + | + | + |
Assimilation: | |||||||||
Glucose | 8 | 2 | 12 | 0 | ̶ | − | + | − | + |
Arabinose | 9 | 0 | 8 | 1 | − | + | + | − | + |
Potassium gluconate | 0 | 0 | 18 | 1 | − | − | − | − | − |
Capric acid | 7 | 1 | 16 | 0 | + | + | − | − | + |
Malic acid | 9 | 4 | 25 | 5 | + | + | − | − | + |
Growth condition NaCl range (%) | |||||||||
0.0–2.0 | 9 | 4 | 25 | 6 | + | + | + | + | + |
3.0 | 9 | 3 | 25 | 5 | + | + | + | + | + |
4.0 | 5 | 0 | 25 | 1 | + | + | +w | + | + |
5.0 | 3 | 0 | 24 | 1 | +w | − | +w | +w | +w |
6.0 | 0 | 0 | 0 | 0 | − | − | − | − | − |
Antisera* | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Titre | S-K604 | S-K575 | S-St5 | S-St16 | S-K530 | S-ATCC BAA 1097 | S-K696 | S-KK3 | S-Am6 | S-TIII | S-ATCC 8071 | S-Sh1 | S-K678 | S-St8 | |
antigens | A-K604 | 2,560 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
A-K575 | 40 | 2,560 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
A-St5 | 0 | 0 | 2,560 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
A-St16 | 0 | 0 | 0 | 2,560 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
A-K530 | 0 | 0 | 0 | 0 | 2,560 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
A- ATCC BAA 1097 | 0 | 0 | 0 | 0 | 0 | 2,560 | 0 | 0 | 0 | 0 | 0 | 160 | 0 | 0 | |
A-K696 | 0 | 0 | 0 | 0 | 0 | 0 | 1,280 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
A-KK3 | 0 | 0 | 0 | 0 | 0 | 160 | 0 | 1,280 | 0 | 0 | 0 | 0 | 0 | 0 | |
A-Am6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1,280 |
0 | 0 | 0 | 0 | ||
A-TIII | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1,280 | 0 | 0 | 0 | 0 | |
A-ATCC 8071 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1,280 | 0 | 0 | 0 | |
A-Sh1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 640 | 0 | 0 | |
A-K678 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 320 | 0 | |
A-St8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 160 | |
Variant | I | II | III | IV | V | VI | VII | VIII | IX | X | XI | XII | XIII | XIV | |
isolates | ID | K604 |
K575 |
St5 |
St16 | K530 | ATCC |
K696 |
KK3 |
Am6 |
TIII |
ATCC |
Sh1 |
K678 | St8 |
n** | 3 | 5 | 3 | 1 | 1 | 1 | 4 | 4 | 2 | 4 | 1 | 3 | 1 | 1 | |
% | 6% | 11% | 6% | 2% | 2% | 2% | 9% | 9% | 4% | 9% | 2% | 6% | 2% | 2% |
Inhibition zone diameter (mm) | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Antimicrobial (concentration) | Bacteria species | 7 | 11 | 14 | 30 | |||||||||||||||||
6 | - | - | - | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | - | >35 | Criteria* | %NWT | ||
10 | 13 | 16 | 35 | |||||||||||||||||||
sulphonamides (300μg) | 24 | 1 | n.a. | n.d. | ||||||||||||||||||
9 | n.d. | |||||||||||||||||||||
4 | n.d. | |||||||||||||||||||||
sp.** |
6 | n.d. | ||||||||||||||||||||
sulphamethoxazole/ trimethoprim (23.75/1.25μg) | 1 | 2 | 3 | 1 | 5 | 3 | 2 | 4 | 4 | NWT≤19 mm | 4 | |||||||||||
3 | 1 | 2 | 1 | 1 | 1 | 33 | ||||||||||||||||
1 | 1 | 1 | 1 | 0 | ||||||||||||||||||
sp.** |
1 | 1 | 1 | 1 | 2 | 0 | ||||||||||||||||
oxolic acid (2μg) | 2 | 3 | 5 | 1 | 1 | 7 | 3 | 1 | 2 | NWT ≤29 mm | 100 | |||||||||||
6 | 1 | 1 | 1 | 100 | ||||||||||||||||||
1 | 1 | 1 | 1 | 75 | ||||||||||||||||||
sp.** |
1 | 1 | 4 | 33 | ||||||||||||||||||
flumequine (30μg) | 1 | 1 | 1 | 5 | 16 | 1 | n.a. | n.d. | ||||||||||||||
5 | 1 | 1 | 1 | 1 | n.d. | |||||||||||||||||
1 | 3 | n.d. | ||||||||||||||||||||
sp.** |
1 | 4 | 1 | n.d. | ||||||||||||||||||
enrofloxacin (5μg) | 1 | 1 | 2 | 1 | 1 | 1 | 2 | 3 | 12 | 1 | n.a. | n.d. | ||||||||||
3 | 2 | 1 | 3 | n.d. | ||||||||||||||||||
1 | 3 | n.d. | ||||||||||||||||||||
sp.** |
1 | 5 | n.d. | |||||||||||||||||||
norfloxacine (10μg) | 2 | 3 | 3 | 1 | 3 | 2 | 1 | 3 | 7 | n.a. | n.d. | |||||||||||
4 | 1 | 1 | 1 | 2 | n.d. | |||||||||||||||||
1 | 1 | 1 | 1 | n.d. | ||||||||||||||||||
sp.** |
1 | 1 | 4 | n.d. | ||||||||||||||||||
oxytetracycline (30μg) | 1 | 1 | 4 | 1 | 7 | 4 | 4 | 1 | 2 | NWT ≤27 mm | 100 | |||||||||||
6 | 2 | 1 | 100 | |||||||||||||||||||
2 | 1 | 1 | 100 | |||||||||||||||||||
sp.** |
1 | 1 | 2 | 1 | 1 | 100 | ||||||||||||||||
doxycycline (30μg) | 2 | 2 | 6 | 3 | 2 | 2 | 1 | 1 | 5 | 1 | n.a. | n.d. | ||||||||||
2 | 1 | 5 | 1 | n.d. | ||||||||||||||||||
1 | 1 | 1 | 1 | n.d. | ||||||||||||||||||
sp.** |
1 | 1 | 1 | 1 | 1 | 1 | n.d. | |||||||||||||||
florphenicol (30μg) | 5 | 16 | 4 | NWT ≤26 mm | 0 | |||||||||||||||||
8 | 1 | 0 | ||||||||||||||||||||
4 | 0 | |||||||||||||||||||||
sp.** |
2 | 1 | 3 | 0 |
Molecular identification | Symbol of isolate | APINE profile | Fish species | Symptoms of diseases/mortality |
---|---|---|---|---|
K736 | 1451354 | Common carp | Yes/Yes | |
K733 | Common carp | No/Yes | ||
KK3 | Koi carp | Yes/Yes | ||
KOI2 | Koi carp | Yes/Yes | ||
W24 | European eel | Yes/Yes | ||
W28 | European eel | Yes/Yes | ||
Jes15 | Siberian sturgeon | No/No | ||
St16 | Slender krib | Yes/Yes | ||
St8 | Slender krib | Yes/Yes | ||
Am6 | Grass carp | No/No | ||
K677 | 1410344 | Common carp | No/No | |
K678 | Common carp | No/No | ||
K769 | Common carp | Yes/No | ||
Pt400 | Rainbow trout | nd/Yes | ||
Pt411 | Rainbow trout | Yes/Yes | ||
K313 | 1450754 | Common carp | Yes/Yes | |
K530 | Common carp | Yes/Yes | ||
K604 | Common carp | Yes/Yes | ||
K679 | Common carp | No/No | ||
K680 | Common carp | No/No | ||
K575 | 1450744 | Common carp | Yes/Yes | |
GIII41(3) | Brown trout | Yes/Yes | ||
St4 | Rainbow trout | Yes/Yes | ||
Tp5 | 1410354 | Silver carp | Yes/Yes | |
Plo1 | Common roach | No/No | ||
Jes34 | Siberian sturgeon | No/No | ||
Tsk20 | 1410754 | Brown trout | Yes/Yes | |
St5 | European whitefish | Yes/Yes | ||
E6 | Rainbow trout | No/No | ||
Sh1 | 1411754 | Common carp | Yes/Yes | |
Sh2 | Common carp | Yes/Yes | ||
Jes1 | 1451754 | Siberian sturgeon | No/No | |
Sh4 | Common carp | Yes/Yes | ||
Jaz4 | Ide | No/No | ||
TIII | 1411744 | Brown trout | Yes/Yes | |
K869 | Common carp | Yes/Yes | ||
K696 | 1450344 | Common carp | Yes/Yes | |
E9 | 1010344 | Rainbow trout | No/No | |
E3 | 1011754 | Rainbow trout | No/No | |
16/c/29 | 1051344 | Zebrafish | Yes/Yes | |
W9 | 1400354 | European eel | Yes/Yes | |
St15 | 1411344 | Slender krib | Yes/Yes | |
K751 | 1450354 | Common carp | No/No | |
GIII46(3) | 1451344 | Brown trout | Yes/Yes |