<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2019-0046_s_005">
<h3>Introduction</h3>
<p>The <italic>Shewanella putrefaciens</italic> group are ubiquitous microorganisms recently isolated from different freshwater fish species and causing serious health disorders. The purpose of the study was to characterise isolates of the <italic>S. putrefaciens</italic> group with special emphasis on elucidating serological diversity and determining putative virulence factors.</p></sec>

<sec id="j_jvetres-2019-0046_s_006">
<h3>Material and Methods</h3>
<p>Isolates collected from freshwater fish (n = 44) and reference strains were used. The identification of bacteria was carried out using biochemical kits and 16S rRNA sequencing. Polyclonal antibodies were prepared against the <italic>S. putrefaciens</italic> group. The bacterium’s susceptibility to antimicrobial agents, its enzymatic properties, and its adhesion ability to fish cell lines were also tested. Finally, selected isolates were used in challenge experiments in common carp and rainbow trout.</p></sec>

<sec id="j_jvetres-2019-0046_s_007">
<h3>Results</h3>
<p>Excluding six isolates undeterminable for species, the bacteria were classified to three species: <italic>S. putrefaciens</italic>, <italic>S. xiamenensis</italic>, and <italic>S. oneidensis</italic>, and showed some phenotypic diversity. Fourteen serological variants of the <italic>S. putrefaciens</italic> group were determined with the newly developed serotyping scheme.</p></sec>

<sec id="j_jvetres-2019-0046_s_008">
<h3>Conclusion</h3>
<p>Serodiversity may play an important role in the virulence of particular isolates. Further, <italic>S. putrefaciens</italic> group members adhere to epithelial cells and produce enzymes which may contribute to their virulence. Challenge tests confirmed the pathogenicity of the <italic>S. putrefaciens</italic> group for fish.</p></sec>

</abstract>

Introduction
The Shewanella putrefaciens group are ubiquitous microorganisms recently isolated from different freshwater fish species and causing serious health disorders. The purpose of the study was to characterise isolates of the S. putrefaciens group with special emphasis on elucidating serological diversity and determining putative virulence factors.


Material and Methods
Isolates collected from freshwater fish (n = 44) and reference strains were used. The identification of bacteria was carried out using biochemical kits and 16S rRNA sequencing. Polyclonal antibodies were prepared against the S. putrefaciens group. The bacterium’s susceptibility to antimicrobial agents, its enzymatic properties, and its adhesion ability to fish cell lines were also tested. Finally, selected isolates were used in challenge experiments in common carp and rainbow trout.


Results
Excluding six isolates undeterminable for species, the bacteria were classified to three species: S. putrefaciens, S. xiamenensis, and S. oneidensis, and showed some phenotypic diversity. Fourteen serological variants of the S. putrefaciens group were determined with the newly developed serotyping scheme.


Conclusion
Serodiversity may play an important role in the virulence of particular isolates. Further, S. putrefaciens group members adhere to epithelial cells and produce enzymes which may contribute to their virulence. Challenge tests confirmed the pathogenicity of the S. putrefaciens group for fish.

Phenotypic diversity and potential virulence factors of the <italic>Shewanella putrefaciens</italic> group isolated from freshwater fish

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phenotypic diversity and potential virulence factors of the  group isolated from freshwater fish

Introduction
The Shewanella putrefaciens group are ubiquitous microorganisms recently isolated from different freshwater fish species and causing serious...

Phenotypic diversity and potential virulence factors of the Shewanella putrefaciens group isolated from freshwater fish

Online veröffentlicht: 13. Sept. 2019

Seitenbereich: 321 - 332

Eingereicht: 07. März 2019

Akzeptiert: 30. Juli 2019

DOI: https://doi.org/10.2478/jvetres-2019-0046

Schlüsselwörter
bacterial fish diseases, serotyping, virulence, adhesion

© 2019 E. Paździor et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phenotypic diversity and potential virulence factors of the Shewanella putrefaciens group isolated from freshwater fish

Online veröffentlicht: 13. Sept. 2019

Seitenbereich: 321 - 332

Eingereicht: 07. März 2019

Akzeptiert: 30. Juli 2019

DOI: https://doi.org/10.2478/jvetres-2019-0046

Schlüsselwörterbacterial fish diseases, serotyping, virulence, adhesion

© 2019 E. Paździor et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
bacterial fish diseases, serotyping, virulence, adhesion