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First Report of Meloidogyne incognita Infecting Mitragyna speciosa in the United States

Alemayehu Habteweld

Alemayehu Habteweld

Entomology and Nematology Department, University of FloridaGainesville, U.S.A
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Habteweld, Alemayehu
, 
Wade Davidson

Wade Davidson

Entomology and Nematology Department, University of FloridaGainesville, U.S.A
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Davidson, Wade
, 
Johan Desaeger

Johan Desaeger

Gulf Coast Research and Education Center, University of FloridaWimauma, U.S.A
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Desaeger, Johan
 und 
William T. Crow

William T. Crow

Entomology and Nematology Department, University of FloridaGainesville, U.S.A
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Crow, William T.
  
02. Juli 2022
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Artikel-Kategorie: Research Note
Online veröffentlicht: 02. Juli 2022
Eingereicht: 08. März 2022
DOI: https://doi.org/10.2478/jofnem-2022-0021
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© 2022 Habteweld et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

(A) Galled kratom (Mitragyna speciosa) root sample received from Hardee County, Florida; (B) a root-knot nematode female inside the kratom root sample.
(A) Galled kratom (Mitragyna speciosa) root sample received from Hardee County, Florida; (B) a root-knot nematode female inside the kratom root sample.

Figure 2

(A) Two mitochondrial DNA regions span the intergenic spacer and part of the adjacent large subunit ribosomal RNA gene (lrDNA) amplified by TRNAH/MHR106 (Lanes 2 and 4, 557-bp) and MORF/MTHIS (Lanes 3 and 5, 742-bp) primer sets for sample 1 (S1) and sample 2 (S2); (B) amplified products using M. incognita-specific (MIF/MIR) (Lanes 2 and 4) and M. javanica-specific (Fjav/Rjav) (Lanes 3 and 5) primers. Only MIF/MIR primers yielded an amplified product approximately 999-bp which indicates the root-knot nematode that infected kratom was M. incognita. No product was observed from Fjav/Rjav primers. Lane 1 = 1 kb DNA ladder, with the position of the 500-bp band indicated by the arrow.
(A) Two mitochondrial DNA regions span the intergenic spacer and part of the adjacent large subunit ribosomal RNA gene (lrDNA) amplified by TRNAH/MHR106 (Lanes 2 and 4, 557-bp) and MORF/MTHIS (Lanes 3 and 5, 742-bp) primer sets for sample 1 (S1) and sample 2 (S2); (B) amplified products using M. incognita-specific (MIF/MIR) (Lanes 2 and 4) and M. javanica-specific (Fjav/Rjav) (Lanes 3 and 5) primers. Only MIF/MIR primers yielded an amplified product approximately 999-bp which indicates the root-knot nematode that infected kratom was M. incognita. No product was observed from Fjav/Rjav primers. Lane 1 = 1 kb DNA ladder, with the position of the 500-bp band indicated by the arrow.
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