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Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities

Phanthiwa Khongkarat
Phanthiwa Khongkarat
, 
Rico Ramadhan
Rico Ramadhan
, 
Preecha Phuwapraisirisan
Preecha Phuwapraisirisan
 und 
Chanpen Chanchao
Chanpen Chanchao
   | 03. März 2021
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Article Category: Original Article
Online veröffentlicht: 03. März 2021
Seitenbereich: 71 - 83
Eingereicht: 11. Juni 2019
Akzeptiert: 09. Nov. 2020
DOI: https://doi.org/10.2478/jas-2021-0001
Schlüsselwörter
© 2021 Phanthiwa Khongkarat et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Summarized procedure for the extraction and enrichment of antioxidants from mimosa flower bee pollen.
Summarized procedure for the extraction and enrichment of antioxidants from mimosa flower bee pollen.

Fig. 2

Morphology of the mimosa flower (Mimosa pigra L.) bee pollen under scanning electron microscopy at 5,000X magnification. Images shown are representative of at least 100 pollen grains observed per sample.
Morphology of the mimosa flower (Mimosa pigra L.) bee pollen under scanning electron microscopy at 5,000X magnification. Images shown are representative of at least 100 pollen grains observed per sample.

Fig. 3

Representative TLC plate showing the compound profile of DCMMBP (lane 1), DCMMBP3-1 (lane 2) and DCMMBP3-2 (lane 3). The mobile phase was 10: 1 (v/v) DCM: MeOH. Arrow indicates compound I.
Representative TLC plate showing the compound profile of DCMMBP (lane 1), DCMMBP3-1 (lane 2) and DCMMBP3-2 (lane 3). The mobile phase was 10: 1 (v/v) DCM: MeOH. Arrow indicates compound I.

Fig. 4

The structural formula of naringenin (Compound I), found in fraction DCMMBP3-2 from DCMMBP3.
The structural formula of naringenin (Compound I), found in fraction DCMMBP3-2 from DCMMBP3.

Characteristics, antioxidant (EC50 value) and anti-tyrosinase (IC50 value) activities of the crude (CME) and partitioned (MTMBP, DCMMBP and HXMBP) bee pollen extracts

Sample Weight (g) Yield (%) Appearance EC50 (μg/mL) IC50 (μg/mL)
Before partition
CME 85.76 61.3 Turbid dark brown oil - -
After partition
MTMBP 51.87 60.5 Dark brown oil >1,000 >500
DCMMBP 7.24 8.44 Sticky dark brown solid 192.1±17.83 >500
HXMBP 8.14 9.49 Dark brown oil >1,000 >500
Reference standards
Ascorbic acid - - - 89.8±5.07 -
Kojic acid - - - - 8.58±0.31

Characteristics, antioxidant (EC50 value) and anti-tyrosinase (% inhibition) activities of the different pooled fractions of DCMMBP after SiG60-CC and Sephadex LH-20 size exclusion CC

Fraction Weight (g) Yield (%) Appearance EC50 (μg/mL) Tyrosinase inhibition (%)
After SiG60-CC
DCMMBP1 0.42 5.8 Sticky dark brown solid 819.5±24.37 2.86±1.75
DCMMBP2 0.69 9.5 Sticky dark brown solid 284.1±11.92 2.99±3.18
DCMMBP3 1.92 26.5 Pale brown solid 112.2±1.35 0
Ascorbic acid - - - 81.1±1.44 -
Kojic acid - - - - 74.2±1.2
After Sephadex LH-20 size exclusion CC
DCMMBP3-1 1.6599 86.5 Pale brown solid 121.3±8.11 -
DCMMBP3-2 0.0176 0.92 Yellow solid >1000 -
Ascorbic acid - - - 81.1±1.44 -
eISSN:
2299-4831
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
2 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Biologie, Zoologie, andere
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