n cysts examined | ||
---|---|---|
Oryx A | 11 (lungs, fertile) | EC (n=10), EO (n=1) |
1 (liver, sterile) | na (n=1) | |
Oryx B | 4 (liver, sterile) | EO (n=2), na (n=2) |
Target | Primers for first PCR (5′-3′) | Primers for nested PCR (5′-3′) |
---|---|---|
Complete |
F: TGGAACTCAGTTTGAGCTTTACTA | F: TATTAAAAATATTGAGTTTGCGTC |
R: ATATCAAAGTAACCTGCTATGCAG | R: TCTTGAAGTTAACAGCATCACGAT | |
~190 bp fragment of |
F: TGTTTTTGAGATCAGTTCGGTGTG | F: CAGTTCGGTGTGCTTTTGGGTCTG |
R: CATAATCAAACGGAGTACGATTAG | R: GAGTACGATTAGTCTCACACAGCA |
Host species | Number of isolated taeniid eggs | Number of successful amplified and sequenced eggs | species identified (number of eggs) |
---|---|---|---|
Dog A | 2 | - | - |
Dog B | 10 | 4 | |
Dog C | 7 | - | - |
Dog D | 10 | - | - |
Dog E | 2 | - | - |
Dog F | 3 | - | - |
Dog G | 2 | - | - |
Dog H | 7 | - | - |
Dog I | 2 | - | - |
Jackal A | - | - | - |
Jackal B | 30 | 6 | |
Jackal C | - | - | - |
Jackal D | 19 | 2 | |
Jackal E | 10 | - | - |