Uneingeschränkter Zugang

The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (Fragaria vesca L.) in vitro

Jurgita Vinskienė

Jurgita Vinskienė

Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and ForestryBabtai, Lithuania
Suche nach diesem Autor auf
Sciendo|Google Scholar
Vinskienė, Jurgita
, 
Vidmantas Bendokas

Vidmantas Bendokas

Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and ForestryBabtai, Lithuania
Suche nach diesem Autor auf
Sciendo|Google Scholar
Bendokas, Vidmantas
, 
Vidmantas Stanys

Vidmantas Stanys

Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and ForestryBabtai, Lithuania
Suche nach diesem Autor auf
Sciendo|Google Scholar
Stanys, Vidmantas
, 
Audrius Sasnauskas

Audrius Sasnauskas

Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and ForestryBabtai, Lithuania
Suche nach diesem Autor auf
Sciendo|Google Scholar
Sasnauskas, Audrius
 und 
Rytis Rugienius

Rytis Rugienius

Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and ForestryBabtai, Lithuania
Suche nach diesem Autor auf
Sciendo|Google Scholar
Rugienius, Rytis
  
31. Dez. 2023
The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (Fragaria vesca L.) in vitro's Cover Image
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zitieren
COVER HERUNTERLADEN
Artikel-Kategorie: ORIGINAL ARTICLE
Online veröffentlicht: 31. Dez. 2023
Seitenbereich: 419 - 431
Eingereicht: 26. Okt. 2022
Akzeptiert: 24. Okt. 2023
DOI: https://doi.org/10.2478/fhort-2023-0030
Schlüsselwörter
© 2023 Jurgita Vinskienė et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1.

Gene expression in wild strawberry microshoots grown on MS medium with different concentrations of sucrose. MS medium with 3% sucrose was used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; MS, Murashige and Skoog; Myb10, MYB domain protein 10; UFGT, UDP glucose: flavonol 3-O-glucosyltransferase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown on MS medium with different concentrations of sucrose. MS medium with 3% sucrose was used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; MS, Murashige and Skoog; Myb10, MYB domain protein 10; UFGT, UDP glucose: flavonol 3-O-glucosyltransferase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Figure 2.

Gene expression in wild strawberry microshoots grown on MS medium with different concentrations of PEG. MS medium with 3% sucrose was used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHS, chalcone synthase; DFR, dihydroflavonol reductase; EGL, enhancer of glabra; MS, Murashige and Skoog; PEG, polyethylene glycol; UFGT, UDP glucose: flavonol 3-O-glucosyltransferase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown on MS medium with different concentrations of PEG. MS medium with 3% sucrose was used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHS, chalcone synthase; DFR, dihydroflavonol reductase; EGL, enhancer of glabra; MS, Murashige and Skoog; PEG, polyethylene glycol; UFGT, UDP glucose: flavonol 3-O-glucosyltransferase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Figure 3.

Wild strawberry microshoots grown under different light spectra: fluorescent light (F), LED lighting system: blue light (B), red light (R), blue + red lights (BR), and blue + red + UV lights (BR UV). LED, light-emitting diode.
Wild strawberry microshoots grown under different light spectra: fluorescent light (F), LED lighting system: blue light (B), red light (R), blue + red lights (BR), and blue + red + UV lights (BR UV). LED, light-emitting diode.

Figure 4.

Gene expression in wild strawberry microshoots grown at 15°C for 1-4 weeks. Microshoots grown at 22°C were used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown at 15°C for 1-4 weeks. Microshoots grown at 22°C were used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Figure 5.

Gene expression in wild strawberry microshoots grown at 30°C for 1-4weeks. Microshoots grown at 22°C were used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol reductase; F3H, flavanone 3-hydroxylase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown at 30°C for 1-4weeks. Microshoots grown at 22°C were used as a control. Bars denote the standard error of the mean. Asterisks indicate significant differences compared to the control assessed by Fisher’s test (*p < 0.05, **p < 0.01), n = 3. CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol reductase; F3H, flavanone 3-hydroxylase; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Supplementary Figure S1.

Gene expression in wild strawberry microshoots grown on MS medium under different light spectra. Microshoots grown under fluorescent lamps were used as a control. Bars denote the standard error of the mean. CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol reductase; F3H, flavanone 3-hydroxylase; MS, Murashige and Skoog; Myb10, MYB domain protein 10; WD40, a tryptophan-aspartic acid (W-D) dipeptide.
Gene expression in wild strawberry microshoots grown on MS medium under different light spectra. Microshoots grown under fluorescent lamps were used as a control. Bars denote the standard error of the mean. CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol reductase; F3H, flavanone 3-hydroxylase; MS, Murashige and Skoog; Myb10, MYB domain protein 10; WD40, a tryptophan-aspartic acid (W-D) dipeptide.

Primers used for anthocyanin biosynthesis gene expression analysis

Gene Accession number Primer sequence Annealing temperature, °C Product size, bp
Myb10 EU155163 F: 5’-CGGAAGATTGCCAGGAAGAAC-3’ 62.4 165
R: 5’-ATGAAGGTTCGTGGTCGAGG-3’ 63.1
WD40-TTG1 XM_004307863 F: 5’-AGCAGGACTTGAGGTACATGG-3’ 63 129
R: 5’-ACGCAATCGCATTCACACTC-3’ 62.8
EGL XM_004308329 F: 5’-GCCTTCGATAAACAAGCGGAAG-3’ 63.1 131
R: 5’-TCTCTATCAGAACCTCCTGCTC-3’ 61.9
UFGT XM_004307828 F: 5’-GCGCATGGTTCAGTTGGAG-3’ 62.8 151
R: 5’-GACCAATCTTCCACACATCCTC-3’ 62
DFR KC894052 F: 5’-GTCTCATTACCGGACTTTCGC-3’ 62.1 131
R: 5’-CTCTGCTTTCGGATGCTCG-3’ 61.9
F3H AB201760 F: 5’-CACAGCAGGTTGTCCATAGC-3’ 62.2 114
R: 5’-AGTGTAAGTCATCGGCTCCTC-3’ 62.6
CHI XM_004307403 5’-AAAGATCAGACCTTCCCACCC-3’ 63 119
R: 5’-TCAATCACCGCATTCCCAAC-3’ 62.4
CHS AB250913 F: 5’-ACTTTTCTGGATTGCACACCC-3’ 62.6 189
R: 5’-GTCTTGTGCCCATTAGCTGC-3’ 62.8
β-actin* XM_004306544 F: 5’-TCAACTATGTTCCCTGGTATTGC-3’ 62 175
R: 5’-CTCCCTTGGAAATCCACATCTG-3’ 62.1

Average weight of wild strawberry microshoots, affected by osmotic components in MS medium, light and growth temperature_

Trait Parameter Average weight of microshoot (g)
Osmotic components Sucrose 15 g ∙ L-1 0.27 ± 0.03
Sucrose 30 g ∙ L-1 0.30 ± 0.03
Sucrose 60 g ∙ L-1 0.13 ± 0.04*
Sucrose 90 g ∙ L-1 0.10 ± 0.04*
Sucrose 30 g ∙ L-1 + PEG 50 g ∙ L-1 0.12 ± 0.10*
Sucrose 30 g ∙ L-1 + PEG 100 g ∙ L-1 0.11 ± 0.11*
Sucrose 30 g ∙ L-1 + PEG 120 g ∙ L-1 0.44 ± 0.60*
Light Fluorescent 0.28 ± 0.01
Blue 0.31 ± 0.02
Red 0.27 ± 0.02
BR 0.34 ± 0.03
BRUV 0.24 ± 0.01
Temperature 15°C 0.13 ± 0.03*
22°C 0.34 ± 0.06
30°C 0.30 ± 0.15
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
2 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Biologie, Botanik, Zoologie, Ökologie, Biologie, andere
Zeitschrift RSS Feed