<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Capsicum chinense</italic> Jacq. is classified under the Solanaceae family, which is an extensively consumed spice and vegetable globally. Therefore, to gain more knowledge and insight into the diversity of <italic>Capsicum chinense</italic> Jacq. metabolites, a total of 18 placental tissues from various development stages were collected and untargeted metabolomics was conducted by means of ultra-performance liquid chromatography (UPLC) and mass spectrometry (MS). Principal component analysis (PCA) analysis established the existence of distinct metabolite distribution patterns as observed at 16 days post anthesis (DPA), compared with the metabolites at 36 and 48 DPA groups, whereas there was a difference in metabolites between the orange ripening period (B) and the red ripening period (C), which intersected with each other. Furthermore, several pathways including metabolic pathways, biosynthesis of phenylpropanoids, ABC transporters, alanine, aspartate and glutamate metabolism, fatty acid biosynthesis, pentose and glucoronate pathways, secondary metabolites biosynthesis, cutin, biosynthesis of suberine and wax were significantly enriched across the fruit ripening stages. The capsaicin content was observed to be less in the early ripening stages, but gradually increased to a high concentration during the late ripening stages. In conclusion, our study findings submit a suitable approach for interpreting the biochemical variances of non-targeted metabolomics in hot pepper developmental stages, as well as offer new findings that can be applied in the development strategies in breeding of <italic>Capsicum chinense</italic> Jacq.</p>
</abstract>

Capsicum chinense Jacq. is classified under the Solanaceae family, which is an extensively consumed spice and vegetable globally. Therefore, to gain more knowledge and insight into the diversity of Capsicum chinense Jacq. metabolites, a total of 18 placental tissues from various development stages were collected and untargeted metabolomics was conducted by means of ultra-performance liquid chromatography (UPLC) and mass spectrometry (MS). Principal component analysis (PCA) analysis established the existence of distinct metabolite distribution patterns as observed at 16 days post anthesis (DPA), compared with the metabolites at 36 and 48 DPA groups, whereas there was a difference in metabolites between the orange ripening period (B) and the red ripening period (C), which intersected with each other. Furthermore, several pathways including metabolic pathways, biosynthesis of phenylpropanoids, ABC transporters, alanine, aspartate and glutamate metabolism, fatty acid biosynthesis, pentose and glucoronate pathways, secondary metabolites biosynthesis, cutin, biosynthesis of suberine and wax were significantly enriched across the fruit ripening stages. The capsaicin content was observed to be less in the early ripening stages, but gradually increased to a high concentration during the late ripening stages. In conclusion, our study findings submit a suitable approach for interpreting the biochemical variances of non-targeted metabolomics in hot pepper developmental stages, as well as offer new findings that can be applied in the development strategies in breeding of Capsicum chinense Jacq.

Biochemical variances through metabolomic profile analysis of <italic>Capsicum chinense</italic> Jacq. during fruit development

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Biochemical variances through metabolomic profile analysis of  Jacq. during fruit development

Capsicum chinense Jacq. is classified under the Solanaceae family, which is an extensively consumed spice and vegetable globally. Therefore, to gain more...

Group name	All diff	Down-regulated	Up-regulated
A vs. B	465	320	145
A vs. C	610	405	205
B vs. C	102	45	57

Group	Diff number	Diff KEGG Annotation
A vs. B	465	27
A vs. C	610	29
B vs. C	102	6

Biochemical variances through metabolomic profile analysis of Capsicum chinense Jacq. during fruit development

Article Category: Research Article

Online veröffentlicht: 02. März 2021

Seitenbereich: 17 - 26

Eingereicht: 11. Aug. 2020

Akzeptiert: 28. Dez. 2020

DOI: https://doi.org/10.2478/fhort-2021-0001

Schlüsselwörter
Jacq., chili pepper, GC-MS, LC-MS, pepper fruit diversity, pepper fruit morphology, untargeted metabolomics

© 2021 Yaping Tang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Results of the differential metabolites (A represents 16 DPA, B represents 36 DPA and C represents 48 DPA).

KEGG annotation results of different metabolites in each group (A represents 16 DPA, B represents 36 DPA and C represents 48 DPA).

Biochemical variances through metabolomic profile analysis of Capsicum chinense Jacq. during fruit development

Article Category: Research Article

Online veröffentlicht: 02. März 2021

Seitenbereich: 17 - 26

Eingereicht: 11. Aug. 2020

Akzeptiert: 28. Dez. 2020

DOI: https://doi.org/10.2478/fhort-2021-0001

SchlüsselwörterJacq., chili pepper, GC-MS, LC-MS, pepper fruit diversity, pepper fruit morphology, untargeted metabolomics

© 2021 Yaping Tang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Results of the differential metabolites (A represents 16 DPA, B represents 36 DPA and C represents 48 DPA).

KEGG annotation results of different metabolites in each group (A represents 16 DPA, B represents 36 DPA and C represents 48 DPA).

Schlüsselwörter
Jacq., chili pepper, GC-MS, LC-MS, pepper fruit diversity, pepper fruit morphology, untargeted metabolomics