Antioxidant enzymatic activity and soluble protein content in Robinia pseudoacacia L. (black locust), invasive tree in Europe, plants were determined under feeding conditions of the caterpillars of Parectopa robiniella Clemens, 1863, invasive miner in Europe, in trees of three (I, II, III) age groups (5–10, 15–25, and 40 years). Biochemical analysis data showed significant differences between reference and experimental leaf samples in different urban ecosystems. There was no association between changes in catalase and peroxidase activity and protein content under the influence of leaf blotch miners depending on the tree age. Significant variability of proteins under P. robiniella influence was noted; the highest of its values were recorded in the leaves of ІІІ-aged trees. In most plantings (and especially in trees of age group III), an increase (1.2 to 2.7 times) in GPx activity or the absence of its changes was registered both in the ecologically favorable zone and in the locations affected by emissions from road transport and industrial enterprises. Plants of age groups I and II in the emission areas of metallurgical, pipe-rolling, and chemical industries experienced a significant decrease in GPx activity (by 20.2–44.6%) in the leaves damaged by the phytophage. In most of the plantings studied, catalase showed an increase/stability of its activity in all age groups of trees under condition of pest attack. The highest increase in САТ activity (2.1 times) was recorded in young R. pseudoacacia plants in an ecologically favorable area of the city.
