<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In the present research we produced triploid, mitogynogenetic (doubled haploid; DH) and meiogynogenetic brook trout (<italic>Salvelinus fontinalis</italic>) to examine efficiency of these technologies and potential susceptibility of chromosome set manipulated individuals for the spinal disorders. Triploidy was induced by shocking (High Hydrostatic Pressure – HHP) of fertilized eggs 30 min. after insemination. In turn, gynogenetic development was induced by activation of eggs with UV-irradiated sperm. Activated eggs were then exposed to HHP shock applied 30 and 420 minutes after insemination to provide meiogynogenotes and gynogenetic DHs, respectively. When compared to non-manipulated diploids, the highest survival rates were observed among triploid brook trout while DHs showed the highest mortality. Malformation rates in the diploid larvae from the control groups did not exceed 7.0% while percentage of malformed triploid individuals equaled 19.1%. Drastically increased number of deformed larvae (> 30%) was observed in both DH and meiogynogenetic individuals. Intensification of kyphosis and scoliosis was clearly demonstrated in the gynogenetic and triploid brook trout. Genetic factors such as increased number of sets of chromosomes in triploids and expression of lethal alleles in the gynogenetic fish plus side effects of HHP shock utilized for retention of the second polar body or inhibition of the first cell cleavage when induced triploid and gynogenetic development have been discussed to affect survival rates and prevalence for the skeletal deformities in the chromosome set manipulated brook trout.</p></abstract>

In the present research we produced triploid, mitogynogenetic (doubled haploid; DH) and meiogynogenetic brook trout (Salvelinus fontinalis) to examine efficiency of these technologies and potential susceptibility of chromosome set manipulated individuals for the spinal disorders. Triploidy was induced by shocking (High Hydrostatic Pressure – HHP) of fertilized eggs 30 min. after insemination. In turn, gynogenetic development was induced by activation of eggs with UV-irradiated sperm. Activated eggs were then exposed to HHP shock applied 30 and 420 minutes after insemination to provide meiogynogenotes and gynogenetic DHs, respectively. When compared to non-manipulated diploids, the highest survival rates were observed among triploid brook trout while DHs showed the highest mortality. Malformation rates in the diploid larvae from the control groups did not exceed 7.0% while percentage of malformed triploid individuals equaled 19.1%. Drastically increased number of deformed larvae (> 30%) was observed in both DH and meiogynogenetic individuals. Intensification of kyphosis and scoliosis was clearly demonstrated in the gynogenetic and triploid brook trout. Genetic factors such as increased number of sets of chromosomes in triploids and expression of lethal alleles in the gynogenetic fish plus side effects of HHP shock utilized for retention of the second polar body or inhibition of the first cell cleavage when induced triploid and gynogenetic development have been discussed to affect survival rates and prevalence for the skeletal deformities in the chromosome set manipulated brook trout.

Production of triploid, doubled haploid (DH) and meiogynogenetic brook trout (<italic>Salvelinus fontinalis</italic>) – efficiency and development of body deformities

Faculty of Oceanography and Geography, Institute of Oceanography, Department of Marine Biology and Ecology

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Production of triploid, doubled haploid (DH) and meiogynogenetic brook trout () – efficiency and development of body deformities

In the present research we produced triploid, mitogynogenetic (doubled haploid; DH) and meiogynogenetic brook trout (Salvelinus fontinalis) to examine...

Production of triploid, doubled haploid (DH) and meiogynogenetic brook trout (Salvelinus fontinalis) – efficiency and development of body deformities

Online veröffentlicht: 29. Jan. 2021

Seitenbereich: 141 - 157

Eingereicht: 27. Nov. 2019

Akzeptiert: 14. Mai 2020

DOI: https://doi.org/10.2478/aoas-2020-0051

Schlüsselwörter
brook trout, gynogenesis, triploids, deformities, scoliosis

© 2020 Krzysztof Jagiełło et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Production of triploid, doubled haploid (DH) and meiogynogenetic brook trout (Salvelinus fontinalis) – efficiency and development of body deformities

Online veröffentlicht: 29. Jan. 2021

Seitenbereich: 141 - 157

Eingereicht: 27. Nov. 2019

Akzeptiert: 14. Mai 2020

DOI: https://doi.org/10.2478/aoas-2020-0051

Schlüsselwörterbrook trout, gynogenesis, triploids, deformities, scoliosis

© 2020 Krzysztof Jagiełło et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
brook trout, gynogenesis, triploids, deformities, scoliosis