RAW 264.7 cell viability (%) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
24 h | 48 h | 72 h | ||||||||||
0.010 µg/mL | 0.025 µg/mL | 0.05 µg/mL | 0.1 µg/mL | 0.010 µg/mL | 0.025 µg/mL | 0.05 µg/mL | 0.1 µg/mL | 0.010 µg/mL | 0.025 µg/mL | 0.05 µg/mL | 0.1 µg/mL | |
88.65±0.09 | 69.05±0.35 | 56.48±0.01 | 89.42±0.02 | 73.98±0.33 | 59.43±0.02 | 86.62±0.23 | 83.49±0.08 | 54.58±0.02 | ||||
81.56±0.26 | 75.43±0.29 | 75.92±0.23 | 74.55±0.23 | 94.62±0.02 | 81.95±0.11 | 71.44±0.03 | 58.43±0.06 | 92.13±0.08 | 91.46±0.34 | 89.06±0.28 | 73.70±0.22 | |
95.30±0.08 | 68.91±0.12 | 62.78±0.25 | 95.75±0.29 | 91.22±0.05 | 85.44±0.06 | 64.09±0.03 | 87.12±0.27 | 82.65±0.09 | 73.35±0.11 | 69.72±0.17 | ||
93.14±0.08 | 80.42±0.19 | 66.66±0.46 | 62.10±0.21 | 88.73±0.06 | 77.09±0.12 | 63.16±0.12 | 56.05±0.04 | 92.13±0.08 | 91.46±0.34 | 89.06±0.28 | 73.71±0.22 | |
90.98±0.30 | 86.81±0.12 | 64.91±0.09 | 60.60±0.31 | 96.51±0.22 | 94.42±0.12 | 80.82±0.08 | 68.49±0.22 | 96.02±0.33 | 87.31±0.27 | 69.95±0.09 | 55.88±0.06 | |
80.46±0.09 | 81.65±0.18 | 85.84±0.26 | 81.47±0.09 | 88.72±0.09 | 72.27±0.04 | 62.23±0.02 | 51.10±0.15 | 85.24±0.09 | 76.56±0.30 | 73.78±0.09 | 56.03±0.32 | |
89.19±0.13 | 78.73±0.17 | 70.83±0.33 | 53.27±0.04 | 92.75±0.13 | 82.93±0.04 | 63.82±0.09 | 54.44±0.05 | 89.70±0.09 | 80.44±0.08 | 63.51±0.35 | 54.76±0.20 | |
78.43±0.31 | 65.99±0.05 | 56.58±0.29 | 50.72±0.04 | 76.23±0.27 | 59.40±0.03 | 53.59±0.06 | 53.05±0.08 | 94.10±0.27 | 79.09±0.03 | 73.90±0.06 | 51.68±0.17 | |
85.54±0.07 | 60.58±0.10 | 56.67±0.26 | 50.07±0.16 | 83.62±0.06 | 62.94±0.12 | 54.81±0.05 | 52.27±0.04 | 91.99±0.42 | 82.25±0.12 | 74.99±0.05 | 54.11±0.09 |
SLN1 | 181.6±1.27 | 0.25±0.02 | -14.6±0.32 | 68.61±0.20 |
SLN2 | 201.8±1.65 | 0.29±0.02 | -17.5±0.14 | 74.53±0.34 |
NLC1 | 176.8±1.90 | 0.57±0.23 | -11.6±0.61 | 77.82±0.21 |
NLC2 | 132.4±1.62 | 0.51±0.53 | -6.3±0.38 | 77.17±0.21 |
Type of LNPs | Baseline value | After one month storage | Baseline value | After one month storage | Baseline value | After one month storage |
---|---|---|---|---|---|---|
SLN1 | 181.6±1.27 | 186.1±2.89 | 0.25±0.02 | 0.36±0.25 | -14.6±0.32 | |
SLN2 | 201.8±1.65 | 200.5±3.56 | 0.29±0.02 | 0.39±0.12 | -17.5±0.14 | |
NLC1 | 176.8±1.90 | 180.3±1.64 | 0.57±0.23 | 0.60±0.46 | -11.6±0.61 | -8.30±0.78 |
NLC2 | 132.4±1.62 | 135.2±3.98 | 0.51±0.53 | 0.65±0.22 | -6.3±0.38 | -5.62±0.96 |
SLN1 | 150 mg | - | - | 100 mg | - | - |
SLN2 | 150 mg | - | - | - | 100 mg | - |
NLC1 | 110 mg | 40 mg | 10 mg | - | 100 mg | 10 mg |
NLC2 | 110 mg | 40 mg | - | - | 100 mg | 10 mg |
Standard MOX solution for SLNs * (0.68 mg) | 33±0.07 | 0.041 |
SLN1 | 0.020 | |
SLN2 | 32±0.11 | 0.041 |
Standard MOX solution for NLCs** (0.78 mg) | 35±0.08 | 0.023 |
NLC1 | 36±0.12 | 0.023 |
NLC2 | 35±0.05 | 0.023 |