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EGF enema and EGFR monoclonal antibody injection alleviate the inflammatory bowel disease in AMO/DSS induced mice model

Xueyan Zheng

Xueyan Zheng

Department of Gastroenterology, First Affiliated Hospital of Fujian Medical UniversityFuzhou, China
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Zheng, Xueyan
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Chengdang Wang

Chengdang Wang

Department of Gastroenterology, First Affiliated Hospital of Fujian Medical UniversityFuzhou, China
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Wang, Chengdang
  
21. Juni 2025
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Artikel-Kategorie: Original Study
Online veröffentlicht: 21. Juni 2025
Seitenbereich: 87 - 94
Eingereicht: 19. Sept. 2024
Akzeptiert: 12. Mai 2025
DOI: https://doi.org/10.2478/ahem-2025-0011
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© 2025 Xueyan Zheng et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

The effect of EGFR mAb treatment combined with EGF enema in the IBD model in vivo. (A) Line-graph showing changes in body weight of mice (n=3 per group) in control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. Treatment time and cycles are mentioned. (B) Bar-graph showing changes in weight of colon tissues (grams) among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. Data is presented as mean ± SEM. * indicates comparison to the initial body weight (Week 0), and # denotes comparison to the body weight at Week 2. ns; non-significant. *P<0.05, **P<0.01, ***P<0.001, # P<0.05, ##P<0.01, ###P<0.001
The effect of EGFR mAb treatment combined with EGF enema in the IBD model in vivo. (A) Line-graph showing changes in body weight of mice (n=3 per group) in control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. Treatment time and cycles are mentioned. (B) Bar-graph showing changes in weight of colon tissues (grams) among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. Data is presented as mean ± SEM. * indicates comparison to the initial body weight (Week 0), and # denotes comparison to the body weight at Week 2. ns; non-significant. *P<0.05, **P<0.01, ***P<0.001, # P<0.05, ##P<0.01, ###P<0.001

Figure 2.

EGFR mAb treatment combined with EGF enema alleviates colonic inflammation in the IBD model in vivo. (A-D) Representative H&E staining images (100x) of colon tissue from control (A), AOM/DSS model (B), model with EGF enema (C) and model with EGF enema and EGFR mAb treatment (D)
EGFR mAb treatment combined with EGF enema alleviates colonic inflammation in the IBD model in vivo. (A-D) Representative H&E staining images (100x) of colon tissue from control (A), AOM/DSS model (B), model with EGF enema (C) and model with EGF enema and EGFR mAb treatment (D)

Figure 3.

EGFR mAb treatment combined with EGF enema reduces proliferation in the IBD model in vivo. (A) IHC images (100x) showing changes in expression of Ki67 and EGFR among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. (B) Bar-graph showing quantification of staining in (A). Data is presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001
EGFR mAb treatment combined with EGF enema reduces proliferation in the IBD model in vivo. (A) IHC images (100x) showing changes in expression of Ki67 and EGFR among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. (B) Bar-graph showing quantification of staining in (A). Data is presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001

Figure 4.

EGFR mAb treatment combined with EGF enema reduces oncogenic signaling in the IBD model in vivo. (A) Immunoblots showing changes in expression of p-AKT, p-ERK and EGFR among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. (B) Bar-graph showing quantification of changes in protein expression in (A). Data is presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001
EGFR mAb treatment combined with EGF enema reduces oncogenic signaling in the IBD model in vivo. (A) Immunoblots showing changes in expression of p-AKT, p-ERK and EGFR among control, AOM/DSS model, model with EGF enema and model with EGF enema and EGFR mAb treatment. (B) Bar-graph showing quantification of changes in protein expression in (A). Data is presented as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001
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Biologie, Molekularbiologie, Mikrobiologie und Virologie, Medizin, Vorklinische Medizin, Grundlagenmedizin, Immunologie
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