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A Study of Phenolic Bioactive Compounds Of Daucus Carota Subsp. Sativus Fruits of Yaskrava, Nantska Kharkivska and Olenka Species and Of Dauci Carotae Subsp. Sativi Fructuum Extractum Siccum


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Figure 1

UPLC-ESI-MS/MS chromatogram of Daucus carota subsp. sativus fruits of Yaskrava species.
UPLC-ESI-MS/MS chromatogram of Daucus carota subsp. sativus fruits of Yaskrava species.

Figure 2

UPLC-ESI-MS/MS chromatogram of Daucus carota subsp. sativus fruits of Olenka species.
UPLC-ESI-MS/MS chromatogram of Daucus carota subsp. sativus fruits of Olenka species.

Figure 3

UPLC-ESI-MS/MS chromatogram of Daucus carota subsp. sativus fruits of Nantska Kharkivska species.
UPLC-ESI-MS/MS chromatogram of Daucus carota subsp. sativus fruits of Nantska Kharkivska species.

Figure 4

UPLC-ESI-MS/MS chromatogram of Dauci carotae subsp. sativi fructus extractum siccum.
UPLC-ESI-MS/MS chromatogram of Dauci carotae subsp. sativi fructus extractum siccum.

0.1% formic acid aqueous solution in acetonitrile, % Time, min
95 1
70 4
50 7
95 2

Bioactive substances of Daucus carota subsp. sativus fruits of Yaskrava, Nantska Kharkivska and Olenka species and of Dauci carotae subsp. sativi fructuum extractum siccum.

Compound m/z of [M H] Daughter ion m/z Content of phenolic compounds as absolutely dry matter, μg/g
Daucus carota subsp. sativus fruits Dauci carotae subsp. sativi fructuum extractum siccum
Yaskrava species Olenka species Nantska Kharkivska species
Quinic acid 191 85 1166.43 ± 29.16 502.83 ±12.57 680.20 ± 17.05 3997.56 ± 99.94
3-(3,4-Dihydroxycinnamoyl) quinic acid (chlorogenic acid) 353 191 129.75 ± 3.24 39.87 ±1.00 242.48 ± 6.62 603.49 ± 15.09
3,4-Dihydroxycinnamic acid (R)-1-carboxy-2-(3,4-dihydroxyphenyl)ethyl ester (rosmarinic acid) 359 161 0.71 ± 0.02 0.57 ± 0.01
3,4-Dihydroxybenzeneacrylic acid (caffeic acid) 392 174 158.91 ± 3.97
Luteolin-7-O-glucoside (cynaroside) 447 285 99.57 ± 2.49 87.48 ± 2.19 80.45 ± 2.01 1997.36 ± 49.93
Quercetin-3-O-rutinoside (rutin) 609 300 14.91 ± 0.37 11.61 ± 0.27 11.50 ± 0.28
Isorhamnetin-3-O-rutinoside (scolymoside) 563 291 6.22 ± 0.16 5.48 ± 0.14 5.10 ± 0.12 124.77 ± 3.12
4′,5,7-Trihydroxyflavone (apigenin) 269 117 0.25 ± 0.01 30.29 ± 0.76
3′-Methylquercetin (isorhamnetin) 315 300 0.06 ± 0.01
Apigenin 8-C-glucoside (vitexin) 431.1 311 0.15 ± 0.01 0.11 ± 0.01 0.10 ± 0.01 4.17 ± 0.10
Phloretin 2′-glucoside (phloridzin) 435 273 0.09 ± 0.01 0.04 ± 0.01
Kaempherol-3-O-glucoside (astragalin) 447 284 3.15 ± 0.08 2.65 ± 0.07 2.68 ± 0.07 67.47 ± 1.69
Quercetin 3-D-galactoside (hyperoside) 451 344 15.73 ± 0.39 11.79 ± 0.29 12.23 ± 0.35 273.92 ± 6.85
Isorhamnetin-3-O-glucoside 477 314 6.86 ± 0.17 6.40 ± 0.16 5.75 ± 0.14 155.89 ± 3.90
3,5,7-Trihydroxyflavone (galangin) 486 195 3.96 ± 0.10 2.99 ± 0.07 2.80 ± 0.07
3′,4′,5,6,7-Pentamethoxyflavone (sinensetin) 414 272 0.10 ± 0.01 0.08 ± 0.01 0.06 ± 0.01
Kaempferol 3-O-(6″-O-p-coumaroyl)glucopyranoside (tiliroside) 528 233 33.05 ± 0.83
Content of alicyclic acids 1166.43 ± 29.16 502.83 ± 12.57 680.20 ± 17.05 3997.56 ± 99.94
Total content of hydroxycinnamic acids 130.46 ± 3.26 40.44 ± 1.01 242.48 ± 6.06 762.40 ± 19.06
Total content of flavonoids 151.05 ± 3.78 128.58 ± 3.21 120.71 ± 3.02 2686.92 ± 67.17
Total content of phenolic compounds 281.51 ± 7.04 169.02 ± 4.23 363.19 ± 9.09 3449.32 ± 86.23
Total content of identified bioactive substances 1447.94 ± 36.20 671.87 ± 16.80 1043.39 ± 26.08 7446.88 ± 186.17
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