<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The purpose of the present work was the evaluation of the prognostic potential of  histopathologic features, cancer stem cells (CSCs), and epthelial-mesenchymal transition (EMT) in relation to lymph node status and lymphovascular invasion (LVI) in canine mammary gland carcinomas (CMGCs). CSCs are proposed as the main cause of tumorigenesis, therapy failure, and recurrence which form a small fraction of tumor bulk. We evaluated presence of micropapillary growth pattern (MGP), infiltration into surrounding tissues (IST), and vasculogenic mimicry (VM) in H&amp;E stained slides of 26 paraffin-embedded tumor samples. Lymph nodes of all cases were assessed. Additionally, they were examined immunohistochemically in terms of  vimentin expression as an indicator of EMT which is a well-known mechanism for metastasis, and CD44, CD24, and ALDH1 for CSCs detection. Data analyses showed significant relationships between MGP and CSCs (P = 0.037), VM and CSCs (P = 0.013), lymph node status and CSCs (P = 0.0001), lymph node status and EMT (P = 0.003), IST and LVI (P = 0.05), VM and LVI (P = 0.01), VM and lymph node status (P = 0.007), and LVI and lymph node status (P = 0.04). Results indicated the prognostic value of MGP, VM, and CSCs with respect to confirmed prognostic markers, including LVI and lymph node involvement, in CMGCs.</p>
</abstract>

The purpose of the present work was the evaluation of the prognostic potential of  histopathologic features, cancer stem cells (CSCs), and epthelial-mesenchymal transition (EMT) in relation to lymph node status and lymphovascular invasion (LVI) in canine mammary gland carcinomas (CMGCs). CSCs are proposed as the main cause of tumorigenesis, therapy failure, and recurrence which form a small fraction of tumor bulk. We evaluated presence of micropapillary growth pattern (MGP), infiltration into surrounding tissues (IST), and vasculogenic mimicry (VM) in H&E stained slides of 26 paraffin-embedded tumor samples. Lymph nodes of all cases were assessed. Additionally, they were examined immunohistochemically in terms of  vimentin expression as an indicator of EMT which is a well-known mechanism for metastasis, and CD44, CD24, and ALDH1 for CSCs detection. Data analyses showed significant relationships between MGP and CSCs (P = 0.037), VM and CSCs (P = 0.013), lymph node status and CSCs (P = 0.0001), lymph node status and EMT (P = 0.003), IST and LVI (P = 0.05), VM and LVI (P = 0.01), VM and lymph node status (P = 0.007), and LVI and lymph node status (P = 0.04). Results indicated the prognostic value of MGP, VM, and CSCs with respect to confirmed prognostic markers, including LVI and lymph node involvement, in CMGCs.

Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas

Department of Pathology and Experimental Animals

Islamic Azad University Central Tehran Branch

Department of Veterinary Laboratory Science

Department of Clinical Science, School of Veterinary Medicine

Department of Medicine, Faculty of Medical Science

Department of Surgery and Radiology, Faculty of Veterinary Medicine

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

The purpose of the present work was the evaluation of the prognostic potential of  histopathologic features, cancer stem cells (CSCs), and...

Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas

Online veröffentlicht: 27. Sept. 2021

Seitenbereich: 303 - 317

Akzeptiert: 23. Aug. 2021

DOI: https://doi.org/10.2478/acve-2021-0026

Schlüsselwörter
cancer stem cells, canine, carcinoma, epithelial-mesenchymal transition, immunohistochemistry, animal model

© 2021 Havva Marzban et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas

Online veröffentlicht: 27. Sept. 2021

Seitenbereich: 303 - 317

Akzeptiert: 23. Aug. 2021

DOI: https://doi.org/10.2478/acve-2021-0026

Schlüsselwörtercancer stem cells, canine, carcinoma, epithelial-mesenchymal transition, immunohistochemistry, animal model

© 2021 Havva Marzban et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
cancer stem cells, canine, carcinoma, epithelial-mesenchymal transition, immunohistochemistry, animal model