<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Until recently, the stem gall nematode <italic>Pterotylenchus cecidogenus</italic> was only registered in eastern Colombia. However, the disease has recently been observed in central Colombian oil palm plantations that use <italic>Desmodium ovalifolium</italic> as a cover crop. Soil, root, stem, and leaf samples were collected from <italic>D. ovalifolium</italic>. Plants showed foliar yellowing, leaf drying, and galls within stem nodes. Nematodes were identified, and the distribution, population density, and relative importance of different genera were determined. We performed morphometric and molecular identification of nematodes associated with gall symptoms. The D2-D3 segment of the large subunit-28S of ribosomal ribonucleic acid (RNA) and internal transcribed spacer (ITS) was sequenced, and phylogenetic analysis was performed. <italic>P. cecidogenus</italic> mainly occurred in the galls and to a lesser extent in the roots and soil. Nematodes were not found in leaf or inflorescence tissue. Morphological and morphometric data confirm the presence of <italic>P. cecidogenus</italic> in the stems of <italic>D. ovalifolium</italic> with gall symptoms. This study is the first to report deoxyribonucleic acid (DNA) sequences of <italic>P. cecidogenus</italic>. Based on D2-D3 and ITS partial sequences, <italic>P. cecidogenus</italic> is a sister species of the leaf-galling nematode <italic>Ditylenchus phyllobius</italic> (Sinm. <italic>Orrina phyllobia</italic>).</p>
</abstract>

Until recently, the stem gall nematode Pterotylenchus cecidogenus was only registered in eastern Colombia. However, the disease has recently been observed in central Colombian oil palm plantations that use Desmodium ovalifolium as a cover crop. Soil, root, stem, and leaf samples were collected from D. ovalifolium. Plants showed foliar yellowing, leaf drying, and galls within stem nodes. Nematodes were identified, and the distribution, population density, and relative importance of different genera were determined. We performed morphometric and molecular identification of nematodes associated with gall symptoms. The D2-D3 segment of the large subunit-28S of ribosomal ribonucleic acid (RNA) and internal transcribed spacer (ITS) was sequenced, and phylogenetic analysis was performed. P. cecidogenus mainly occurred in the galls and to a lesser extent in the roots and soil. Nematodes were not found in leaf or inflorescence tissue. Morphological and morphometric data confirm the presence of P. cecidogenus in the stems of D. ovalifolium with gall symptoms. This study is the first to report deoxyribonucleic acid (DNA) sequences of P. cecidogenus. Based on D2-D3 and ITS partial sequences, P. cecidogenus is a sister species of the leaf-galling nematode Ditylenchus phyllobius (Sinm. Orrina phyllobia).

<article-title xml:lang="en">Characterization of <italic>Pterotylenchus cecidogenus</italic> in <italic>Desmodium ovalifolium</italic> cover crop from oil palm plantations in central Colombia

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of  in  cover crop from oil palm plantations in central Colombia

Until recently, the stem gall nematode Pterotylenchus cecidogenus was only registered in eastern Colombia. However, the disease has recently been observed in...

Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia

Online veröffentlicht: 01. Dez. 2021

Seitenbereich: 1 - 14

Eingereicht: 16. Nov. 2020

DOI: https://doi.org/10.21307/jofnem-2021-099

Schlüsselwörter
Diagnostic, Molecular biology, Phylogenetic analysis, Stem gall

© 2021 Oscar Velandia et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of Pterotylenchus cecidogenus in Desmodium ovalifolium cover crop from oil palm plantations in central Colombia

Online veröffentlicht: 01. Dez. 2021

Seitenbereich: 1 - 14

Eingereicht: 16. Nov. 2020

DOI: https://doi.org/10.21307/jofnem-2021-099

SchlüsselwörterDiagnostic, Molecular biology, Phylogenetic analysis, Stem gall

© 2021 Oscar Velandia et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Schlüsselwörter
Diagnostic, Molecular biology, Phylogenetic analysis, Stem gall