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Interaction of agonists of a different subtype of the nAChR and carvacrol with GABA in Ascaris suum somatic muscle contractions

Djordje S. Marjanović
Djordje S. Marjanović
, 
Saša M. Trailović
Saša M. Trailović
 und 
Mirjana Milovanović
Mirjana Milovanović
   | 01. Jan. 2021
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Online veröffentlicht: 01. Jan. 2021
Seitenbereich: 1 - 8
DOI: https://doi.org/10.21307/jofnem-2021-022
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© 2021 Authors, published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

A Original recording of isometric contractions of A. suum muscle flap induced by increasing concentrations of pyrantel and the effect of GABA (3 μM) and Carvacrol (100 μM) on those contractions; B the concentration-response plot for pyrantel control (n = 6, blue), in the presence of GABA 3 μM (n = 6, green), in the presence of GABA and Carvacrol 100 μM (n = 6, red) and after washing (n = 6, dashed black) (mean ± S.E.).
A Original recording of isometric contractions of A. suum muscle flap induced by increasing concentrations of pyrantel and the effect of GABA (3 μM) and Carvacrol (100 μM) on those contractions; B the concentration-response plot for pyrantel control (n = 6, blue), in the presence of GABA 3 μM (n = 6, green), in the presence of GABA and Carvacrol 100 μM (n = 6, red) and after washing (n = 6, dashed black) (mean ± S.E.).

Figure 2:

A Original recording of isometric contractions of A. suum muscle flap induced by increasing concentrations of bephenium and the effect of GABA (3 μM) and Carvacrol (100 μM) on those contractions; B the concentration-response plot for bephenium control (n = 6, blue), in the presence of GABA 3 μM (n = 6, green), in the presence of GABA and Carvacrol 100 μM (n = 6, red) and after washing (n = 6, dashed black) (mean ± S.E.).
A Original recording of isometric contractions of A. suum muscle flap induced by increasing concentrations of bephenium and the effect of GABA (3 μM) and Carvacrol (100 μM) on those contractions; B the concentration-response plot for bephenium control (n = 6, blue), in the presence of GABA 3 μM (n = 6, green), in the presence of GABA and Carvacrol 100 μM (n = 6, red) and after washing (n = 6, dashed black) (mean ± S.E.).

Figure 3:

A Original recording of isometric contractions of A. suum muscle flap induced by increasing concentrations of morantel and the effect of GABA (3 μM) and carvacrol (100 μM) on those contractions; B the concentration-response plot for morantel control (n = 6, blue), in the presence of GABA 3 μM (n = 6, green), in the presence of GABA and carvacrol 100 μM (n = 6, red) and after washing (n = 6, dashed black) (mean ± S.E.).
A Original recording of isometric contractions of A. suum muscle flap induced by increasing concentrations of morantel and the effect of GABA (3 μM) and carvacrol (100 μM) on those contractions; B the concentration-response plot for morantel control (n = 6, blue), in the presence of GABA 3 μM (n = 6, green), in the presence of GABA and carvacrol 100 μM (n = 6, red) and after washing (n = 6, dashed black) (mean ± S.E.).

EC50 (±S.E.) values of pyrantel, bephenium, and morantel in the presence of GABA and carvacrol.

Control GABA 3 μM GABA 3 μM + Carvacrol 100 μM Wash
Pyrantel (nM) 8.44 ± 1.56 28.11 ± 1.61**** 44.62 ± 2.70****++++ 20.51 ± 2.34***oooo
Bephenium (μM) 0.62 ± 0.11 0.96 ± 0.21 1.40 ± 0.12*** 1.32 ± 0.09**
Morantel (nM) 3.72 ± 1.73 5.69 ± 2.12 7.94 ± 2.35 15.81 ± 4.03*+

R max (g ± S.E.) values of pyrantel, bephenium, and morantel in the presence of GABA and carvacrol.

Control GABA 3 μM GABA 3 μM + Carvacrol 100 μM Wash
Pyrantel 1.52 ± 0.12 1.38 ± 0.20 0.47 ± 0.15****+++ 0.77 ± 0.17**+
Bephenium 2.20 ± 0.30 2.18 ± 0.34 0.89 ± 0.22**++ 1.15 ± 0.23*+
Morantel 1.16 ± 0.07 0.99 ± 0.11 0.39 ± 0.11****+++ 0.59 ± 0.15***o
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