<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme importance for the assessment of iron in samples, which can then be used for further investigations. Biological samples are a good example, however, as the concentrations of iron are very low in these, it is important to exclude possible artefacts from the background spectrum related to iron present in the counter and cryostat windows. The aim of this study was to compare two methods of determination of the amounts of iron in investigated sample: one, in which the background spectrum was subtracted from the sample spectrum measured, and the other, in which the obtained non-elaborated spectrum was fitted with two doublets - a doublet for the measured sample and a doublet for the background spectrum. Three samples containing known amounts of natural iron (400, 800 and 1600 μg) and a sample of lyophilized human brain tissue obtained from globus pallidus were assessed. Both methods led to the creation of a very good calibration curve with a correlation coefficient of 0.99. Although both methods gave similar results for the concentration of iron in the sample, the subtraction of the background spectrum had a significantly lower error of the final result. </p></abstract>

Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme importance for the assessment of iron in samples, which can then be used for further investigations. Biological samples are a good example, however, as the concentrations of iron are very low in these, it is important to exclude possible artefacts from the background spectrum related to iron present in the counter and cryostat windows. The aim of this study was to compare two methods of determination of the amounts of iron in investigated sample: one, in which the background spectrum was subtracted from the sample spectrum measured, and the other, in which the obtained non-elaborated spectrum was fitted with two doublets - a doublet for the measured sample and a doublet for the background spectrum. Three samples containing known amounts of natural iron (400, 800 and 1600 μg) and a sample of lyophilized human brain tissue obtained from globus pallidus were assessed. Both methods led to the creation of a very good calibration curve with a correlation coefficient of 0.99. Although both methods gave similar results for the concentration of iron in the sample, the subtraction of the background spectrum had a significantly lower error of the final result.

An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue

Nukleonika

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of...

An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue

Online veröffentlicht: 09. Juni 2017

Seitenbereich: 159 - 163

Eingereicht: 02. Juli 2016

Akzeptiert: 29. Nov. 2016

DOI: https://doi.org/10.1515/nuka-2017-0024

Schlüsselwörter
Mössbauer spectroscopy, background spectrum, iron concentration, brain tissue, calibration curve

© by Jolanta Gałązka-Friedman

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue

Online veröffentlicht: 09. Juni 2017

Seitenbereich: 159 - 163

Eingereicht: 02. Juli 2016

Akzeptiert: 29. Nov. 2016

DOI: https://doi.org/10.1515/nuka-2017-0024

SchlüsselwörterMössbauer spectroscopy, background spectrum, iron concentration, brain tissue, calibration curve

© by Jolanta Gałązka-Friedman

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Schlüsselwörter
Mössbauer spectroscopy, background spectrum, iron concentration, brain tissue, calibration curve