<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Free radicals formed during thermal sterilization of <italic>eucerinum anhydricum</italic> – the pharmaceutical base were examined by an X-band (9.3 GHz) spectrometer. <italic>Eucerinum anhydricum</italic> was sterilized at different physical conditions according to the Polish Pharmacopeia norms. The samples were heated at temperatures: 160°C (120 min), 170°C (60 min), and 180°C (30 min). The aim of this study is to compare free radical concentration and effect of microwave power on EPR spectra of <italic>eucerinum anhydricum</italic> base thermally sterilized at different temperatures and periods of time. The effect of time storage on the free radicals in the heated samples was tested. Free radical concentrations in the sample stored 15 min strongly decreased with the increasing of sterilization temperature, probably as the result of recombination. Storage caused strong decrease of free radical concentrations in the samples, probably as the result of interactions with oxygen. It was observed to be independent of sterilization conditions from 2 days of storage and longer. Because of the lowest free radical concentration, for <italic>eucerinum anhydricum</italic> thermal sterilization at 180°C for 30 min is recommended. The sterilized samples should be stored at inert atmosphere without oxygen molecules. Fast spin-lattice relaxation processes existed in sterilized <italic>eucerinum anhydricum</italic>. The character of changes of amplitudes and linewidths of EPR lines with increasing of microwave power was the same for different storage times. The parameters of thermal sterilization and storage time influenced free radical concentration in <italic>eucerinum anhydricum</italic>, but magnetic spin-lattice interactions were unchanged. The usefulness of EPR spectroscopy in optimization of thermal sterilization process of <italic>eucerinum anhydricum</italic> was confirmed.</p></abstract>

Free radicals formed during thermal sterilization of eucerinum anhydricum – the pharmaceutical base were examined by an X-band (9.3 GHz) spectrometer. Eucerinum anhydricum was sterilized at different physical conditions according to the Polish Pharmacopeia norms. The samples were heated at temperatures: 160°C (120 min), 170°C (60 min), and 180°C (30 min). The aim of this study is to compare free radical concentration and effect of microwave power on EPR spectra of eucerinum anhydricum base thermally sterilized at different temperatures and periods of time. The effect of time storage on the free radicals in the heated samples was tested. Free radical concentrations in the sample stored 15 min strongly decreased with the increasing of sterilization temperature, probably as the result of recombination. Storage caused strong decrease of free radical concentrations in the samples, probably as the result of interactions with oxygen. It was observed to be independent of sterilization conditions from 2 days of storage and longer. Because of the lowest free radical concentration, for eucerinum anhydricum thermal sterilization at 180°C for 30 min is recommended. The sterilized samples should be stored at inert atmosphere without oxygen molecules. Fast spin-lattice relaxation processes existed in sterilized eucerinum anhydricum. The character of changes of amplitudes and linewidths of EPR lines with increasing of microwave power was the same for different storage times. The parameters of thermal sterilization and storage time influenced free radical concentration in eucerinum anhydricum, but magnetic spin-lattice interactions were unchanged. The usefulness of EPR spectroscopy in optimization of thermal sterilization process of eucerinum anhydricum was confirmed.

Effect of microwave power on EPR spectra of thermally sterilized <italic>eucerinum anhydricum</italic>

Department of Biophysics, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, 8 Jedności Str., 41-200 Sosnowiec, Poland

Nukleonika

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of microwave power on EPR spectra of thermally sterilized 

Free radicals formed during thermal sterilization of eucerinum anhydricum – the pharmaceutical base were examined by an X-band (9.3 GHz) spectrometer....

Effect of microwave power on EPR spectra of thermally sterilized eucerinum anhydricum

Online veröffentlicht: 06. Aug. 2015

Seitenbereich: 439 - 442

Eingereicht: 11. Sept. 2014

Akzeptiert: 30. Jan. 2015

DOI: https://doi.org/10.1515/nuka-2015-0078

Schlüsselwörter
EPR, microwave saturation, free radicals, thermal sterilization

© Paweł Ramos et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of microwave power on EPR spectra of thermally sterilized eucerinum anhydricum

Online veröffentlicht: 06. Aug. 2015

Seitenbereich: 439 - 442

Eingereicht: 11. Sept. 2014

Akzeptiert: 30. Jan. 2015

DOI: https://doi.org/10.1515/nuka-2015-0078

SchlüsselwörterEPR, microwave saturation, free radicals, thermal sterilization

© Paweł Ramos et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
EPR, microwave saturation, free radicals, thermal sterilization