<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Capsicum annuum</italic> L. CM334 is susceptible to <italic>Nacobbus aberrans</italic> but highly resistant to <italic>Phy-tophthora capsici.</italic> Resistance to <italic>P. capsici</italic> is associated with the over-expression of various defense genes such as those encoding pathogenesis-related proteins. The transcriptional alterations of defense-related genes were determined in galls induced by <italic>N. aberrans (Na)</italic> in CM334 chili roots. Transcripts accumulation of <italic>WRKY-a, WRKY1, POX</italic> (peroxidase), <italic>PR-1</italic> (pathogenesis-related protein 1), and <italic>EAS</italic> (5-epiaristolochene synthase) was estimated by qRT-PCR, and they were compared with those recorded in the incompatible CM334- <italic>P. capsici (Pc)</italic> interaction. The levels of all studied genes were significantly (P s 0.05) lower <italic>(WRKY1, POX</italic> and <italic>PR-1)</italic> or down-regulated <italic>(WRKY-a</italic> and EAS) in the presence of <italic>N. aberrans</italic>; in contrast, in the incompatible interaction, all genes were significantly up-regulated. The alterations induced by <italic>N. aberrans</italic> could be necessary to ensure the successful completion of its life cycle in CM334 chili roots.</p></abstract>

Capsicum annuum L. CM334 is susceptible to Nacobbus aberrans but highly resistant to Phy-tophthora capsici. Resistance to P. capsici is associated with the over-expression of various defense genes such as those encoding pathogenesis-related proteins. The transcriptional alterations of defense-related genes were determined in galls induced by N. aberrans (Na) in CM334 chili roots. Transcripts accumulation of WRKY-a, WRKY1, POX (peroxidase), PR-1 (pathogenesis-related protein 1), and EAS (5-epiaristolochene synthase) was estimated by qRT-PCR, and they were compared with those recorded in the incompatible CM334- P. capsici (Pc) interaction. The levels of all studied genes were significantly (P s 0.05) lower (WRKY1, POX and PR-1) or down-regulated (WRKY-a and EAS) in the presence of N. aberrans; in contrast, in the incompatible interaction, all genes were significantly up-regulated. The alterations induced by N. aberrans could be necessary to ensure the successful completion of its life cycle in CM334 chili roots.

Defense gene expression in root galls induced by  <italic>Nacobbus aberrans</italic> in CM334 chilli plants

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Defense gene expression in root galls induced by   in CM334 chilli plants

Capsicum annuum L. CM334 is susceptible to Nacobbus aberrans but highly resistant to Phy-tophthora capsici. Resistance to P. capsici is associated with the...

Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants

Online veröffentlicht: 02. Feb. 2015

Seitenbereich: 77 - 82

Eingereicht: 10. Nov. 2014

Akzeptiert: 09. Okt. 2014

DOI: https://doi.org/10.1515/helmin-2015-0014

Schlüsselwörter
qRT-PCR, transcription factors, gene reprogramming

© 2015 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants

Online veröffentlicht: 02. Feb. 2015

Seitenbereich: 77 - 82

Eingereicht: 10. Nov. 2014

Akzeptiert: 09. Okt. 2014

DOI: https://doi.org/10.1515/helmin-2015-0014

SchlüsselwörterqRT-PCR, transcription factors, gene reprogramming

© 2015 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
qRT-PCR, transcription factors, gene reprogramming