<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In this work, the effects of sodium dodecyl sulfate (SDS) concentrations on retention, separation selectivity, peak shapes and systems efficiency were investigated. Herein, the retention data for 11 alkaloids were determined on an RP18 silica column with mobile phases containing methanol as organic modifier, with acetate buffer at pH 3.5, and, subsequently, with the addition of sodium dodecyl sulfate (SDS). The results of this study indicate that the retention of alkaloids decreases with the increase of SDS concentration in the mobile phase. The increase of SDS concentration, however, leads to the significantly improvement of peak symmetry and the increase of theoretical plate number in all cases. The best system efficiency for most of the investigated alkaloids was obtained in a mobile phase containing 0.1 M SDS, while most symmetrical peaks were obtained through the addition of 0.3 M of SDS to the mobile phase.</p></abstract>

In this work, the effects of sodium dodecyl sulfate (SDS) concentrations on retention, separation selectivity, peak shapes and systems efficiency were investigated. Herein, the retention data for 11 alkaloids were determined on an RP18 silica column with mobile phases containing methanol as organic modifier, with acetate buffer at pH 3.5, and, subsequently, with the addition of sodium dodecyl sulfate (SDS). The results of this study indicate that the retention of alkaloids decreases with the increase of SDS concentration in the mobile phase. The increase of SDS concentration, however, leads to the significantly improvement of peak symmetry and the increase of theoretical plate number in all cases. The best system efficiency for most of the investigated alkaloids was obtained in a mobile phase containing 0.1 M SDS, while most symmetrical peaks were obtained through the addition of 0.3 M of SDS to the mobile phase.

Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

Department of Inorganic Chemistry Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland

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This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In this work, the effects of sodium dodecyl sulfate (SDS) concentrations on retention, separation selectivity, peak shapes and systems efficiency were...

Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

Online veröffentlicht: 16. Juli 2015

Seitenbereich: 126 - 130

Eingereicht: 31. März 2015

Akzeptiert: 08. Mai 2015

DOI: https://doi.org/10.1515/cipms-2015-0058

Schlüsselwörter
alkaloids, HPLC, micellar chromatography, SDS, system efficiency

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

Online veröffentlicht: 16. Juli 2015

Seitenbereich: 126 - 130

Eingereicht: 31. März 2015

Akzeptiert: 08. Mai 2015

DOI: https://doi.org/10.1515/cipms-2015-0058

Schlüsselwörteralkaloids, HPLC, micellar chromatography, SDS, system efficiency

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
alkaloids, HPLC, micellar chromatography, SDS, system efficiency