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A simplified maturity index to quantify the development stage of perennial ryegrass (Lolium perenne L.) and its relationship with yield and nutritive value


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Classification of Lolium perenne L. developmental stages according to Park (1980)Tabelle 3. Klassifizierung des Entwicklungsstadiums von Lolium perenne L. nach Park (1980)

CodeAbbreviationDescription
1S0No elongated leaf sheath
2S11 elongated leaf sheath (1 fully developed leaf)
3S22 elongated leaf sheaths (2 fully developed leafs)
4S33 or more elongated leaf sheaths (3 fully developed leafs)
5K11 tactile node
6K22 tactile nodes
7K33 or more tactile nodes
8BSwelling of the upper leaf sheath indicating presence of the inflorescence inside it (boot stage)
9G1.1Beginning of heading, the upper 1-2 cm of the inflorescence is visible
10G1.550 % of the inflorescence emerged
11G1.9The inflorescence fully emerged and the inflorescence peduncle is visible
12OHInflorescence peduncle fully emerged
13G2.1Beginning of flowering (anthesis), some anthers are visible
14G2.5Full flowering, maximum number of anthers are visible
15G2.9End of flowering, only few anthers are still visible
16G3Milk ripe
17G4Dough ripe

Means of ADL and DOM contents (g kg-1) of the 20 tested genotypes for the two cuts as analyzed with and without the covariable (SMI8)Tabelle 9. Mittlere Gehalte für ADL und VOM (g kg-1) der 20 untersuchten Sorten aus 2 Aufwüchsen mit und ohne Berücksichtigung der Kovariable (SMI8)

ADLDOM
GenotypeWithout SMI8With SMI8Without SMI8With SMI8
Cut 1Cut 2Cut 1Cut 2Cut 1Cut 2Cut 1Cut 2
116.7 B a21.0 A ab16.2 B ab20.9 A ab803.0 A abc

Within parameters and within each set of means comparison, different capital letter points out significant difference between the two cuts for the same genotype, while, different small letter(s) point out significant difference(s) among the twenty genotypes within each cut according to the LSD test at 0.05 level of probability. Data were averaged over years and sampling dates. SMI8 represents the percentage of tillers above the boot stage.

752.1 B abcde807.2 A ab752.3 B abcde
216.9 B a20.1 A ab16.7 B a20.5 A ab803.2 A abc762.6 B ab805.0 A ab759.2 B abc
315.1 B abc22.8 A ab15.2 B ab22.5 A ab813.2 A abc732.3 B cde812.3 A ab735.2 B cde
412.0 B c20.8 A ab12.5 B b20.6 A ab832.6 A a745.5 B bcde828.3 A a747.5 B bcde
517.9 B a21.3 A ab17.2 B a21.3 A ab793.5 A bc746.8 B bcde799.8 A ab746.8 B bcde
618.0 B a20.9 A ab17.2 B a21.0 A ab785.6 A c755.1 B abcde792.4 A b754.6 B abcde
714.6 B abc22.1 A ab15.2 B ab22.1 A ab830.2 A a748.3 B bcde825.2 A ab748.4 B bcde
813.9 B abc21.1 A ab14.3 B ab21.3 A ab830.0 A a745.4 B bcde826.0 A a743.8 B bcde
912.4 B bc18.1 A b12.7 B b18.6 A b833.7 A a780.6 B a831.3 A a776.4 B a
1015.0 B abc18.5 A b14.8 B ab18.6 A b809.7 A abc778.6 B a811.6 A ab777.7 B a
1115.7 B abc20.3 A ab15.9 B ab20.7 A ab813.9 A abc764.8 B ab812.6 A ab761.6 B abc
1214.6 B abc20.9 A ab14.9 B ab20.8 A ab824.8 A ab751.7 B abcde822.0 A ab752.1 B abcde
1314.7 B abc19.1 A b14.9 B ab19.2 A b816.8 A abc765.8 B ab814.4 A ab764.6 B ab
1416.6 B ab21.1 A ab16.2 B ab21.2 A ab807.9 A abc759.3 B abc811.2 A ab759.1 B abc
1515.2 B abc24.8 A a15.3 B ab24.2 A a817.1 A abc726.0 B e816.8 A ab731.4 B de
1614.8 B abc21.5 A ab15.0 B ab21.2 A ab820.8 A ab753.0 B abcde819.3 A ab755.4 B abcd
1716.7 B a20.4 A ab16.3 B ab20.7 A ab804.9 A abc767.9 B ab808.2 A ab765.0 B ab
1815.0 B abc24.2 A a15.2 B ab24.1 A a827.4 A ab727.7 B de825.6 A a728.9 B e
1916.0 A abc18.5 A b15.9 B ab18.9 A b807.6 A abc772.1 B ab808.3 A ab768.6 B ab
2017.5 B a20.7 A ab17.0 B a20.6 A ab802.0 A abc757.1 B abcd806.9 A ab757.7 B abcd
Min.12.018.112.518.6785.6726.0792.4728.9
Max.18.024.817.224.2833.7780.6831.3777.7
Range6.06.74.75.648.154.638.948.8
S.E.0.850.825.75.5

Harvesting schedule for the two experimental seasonsTabelle 2. Erntetermine für die zwei Versuchsjahre

CutSampling numberYear 1Year 2
115 May08 May
1229 May13 May
309 June23 May
128 June18 June
2203 July22 June
311 July03 July
3123 Aug.17 Aug.
4110 Oct.10 Oct.

Genotypes included in the studyTabelle 1. Liste der in der Studie verwendeten Sorten

GenotypeHeading date

the heading date is provided as number of days after April 1st

Registration year
1541993
2561997
3581987
4631995
553

not registered

653

not registered

762

not registered

863

not registered

9612005
10551999
11602003
12622004
13632009
14552008
1561

not registered

1664

not registered

17542007
1855

not registered

19552007
20562007

Statistical data of the calibration of NIRS for NDF, ADF, ADL and DOM of the investigated genotypesTabelle 4. NIRS Kalibrationsstatistik für NDF, ADF, ADL und VOM der untersuchten Sorten

Parameter (g kg-1)n

n: number of samples; SEC: Standard error of calibration; SEV: Standard error of validation

MeanRangeR2SECSEV
NDF96566.5413.9-692.60.9316.0020.79
ADF92280.0173.5-362.70.977.0310.28
ADL6320.03.7-41.10.545.605.32
DOM99768.7610.0-897.10.989.9015.50

Spearman’s rank correlation coefficients between the tested maturity indices, i.e., Mean stage by count (MSC) and Simple maturity indices SMIi (i = 3-12), and forage quality parametersTabelle 5. Spearman Korrelationskoeffizienten zwischen den getesteten Indices, d.h. “mean stage by count” (MSC) und “simply maturity indices” SMIi (i = 3-12), und Futterqualitätsparameter

SMIi
CurSampling dateParameterMSC3456789101112
S2S3K1K2K3BGl.lG1.5G1.9OH
11NDF0.32**0.040.100.36**0.31*0.21*0.22*0.17
11ADF0.34**0.060.140.37**0.32*0.20*0.24*0.22*
11ADL0.54**0.030.180.48**0.55**0.48**0.44**0.40**
11DOM-0.84**-0.03-0.49**-0.85**-0.82**-0.69**-0.74**-0.59**
11Yield0.040.070.030.010.090.070.020.1
12NDF0.57**0.060.54**0.48**0.59**0.52**0.62**0.58**0.57**
12ADF0.67**0.110.62**0.56**0.68**0.62**0.68**0.59**0.61**
12ADL0.51**0.050.44**0.42**0.52**0.47**0.63**0.55**0.58**
12DOM-0.69**-0.08-0.59**-0.53**-0.72**-0.65**-0.68**-0.64**-0.69**
12Yield0.340.070.160.390.40*0.390.230.220.08
13NDF0.78**0.22*0.25*0.48**0.70**0.76**0.81**0.67**0.64**0.39**
13ADF0.72**0.31*0.32**0.53**0.72**0.74**0.74**0.55**0.56**0.59**
13ADL0.83**0.21*0.190.37**0.59**0.70**0.83**0.79**0.77**0.55**
13DOM-0.81**-0.23*-0.21*-0.39**-0.63**-0.75**-0.81**-0.73**-0.73**-0.54**
13Yield0.260.71**0.370.370.49*0.40*0.210.040.040.0002
21NDF0.47**0.140.21*0.43**0.39**0.33**0.39**0.49**0.55**0.56**0.40**
21ADF0.39**0.170.21*0.32**0.27*0.23*0.32**0.43**0.50**0.49**0.32**
21ADL0.48**0.170.31*0.54**0.49**0.40**0.38**0.41**0.46**0.46**0.31*
21DOM-0.51**-0.12-0.26*-0.44**-0.41**-0.37**-0.41**-0.56**-0.66**-0.65**-0.51**
21Yield0.120.020.020.060.040.030.040.190.33**0.28*0.28*
22NDF0.31*0.040.0370.20*0.22*0.26*0.28*0.36**0.40**0.21*0.19
22ADF0.33**0.180.080.170.180.25*0.34**0.37**0.38**0.20*0.21*
22ADL0.47**0.120.110.34**0.36**0.37**0.31*0.39**0.49**0.36**0.31*
22DOM-0.35**-0.10-0.05-0.28*-0.28*-0.30*-0.26*-0.33**-0.42**-0.24*-0.23*
22Yield0.0030.0060.0050.0020.0020.020.030.020.0040.0070.004
23NDF0.180.030.20*0.090.130.170.180.26*0.29*0.160.09
23ADF0.180.0010.20*0.090.160.180.20*0.27*0.30*0.170.09
23ADL0.21*0.00060.24*0.160.20*0.23*0.23*0.28*0.33**0.22*0.15
23DOM-0.40**-0.01-0.39**-0.28*-0.37**-0.41**-0.43**-0.50**-0.54**-0.41**-0.24*
23Yield0.140.00040.150.21*0.21*0.180.160.130.140.170.35**

Means of NDF and ADF contents (g kg-1) of the 20 tested genotypes for the two cuts as analyzed with and without the covariable (SMI8)Tabelle 8. Mittlere Gehalte für NDF und ADF (g kg-1) der 20 untersuchten Sorten aus 2 Aufwüchsen mit und ohne Berücksichtigung der Kovariable (SMI8)

NDFADF
GenotypeWithout SMI8With SMI8Without SMI8With SMI8
Cut 1Cut 2Cut 1Cut 2Cut 1Cut 2Cut 1Cut 2
1517.2 B abc

Within parameters and within each set of means comparison, a different capital letter points out significant difference between the two cuts for the same genotype, while, different small letter(s) point out significant difference(s) among the twenty genotypes within each cut according to the LSD test at 0.05 level of probability. Data were averaged over years and sampling dates. SMI8 represents the percentage of tillers above the boot stage.

587.0 A abcd513.8 B a586.8 A abcd247.3 B ab290.3 A abc244.4 B a290.2 A abc
2536.5 B ab576.7 A bcde535.1 B a579.4 A bcde263.4 B a285.2 A bc262.2 B a287.5 A abc
3511.9 B abc596.5 A ab512.7 B a594.3 A abc241.8 B ab293.5 A abc242.4 B a291.6 A abc
4484.0 B bc587.2 A abcd487.4 B a585.6 A abcd223.3 B b291.3 A abc226.3 B a290.0 A abc
5538.7 B a593.5 A abc533.7 B a593.6 A abc259.4 B ab295.5 A abc255.1 B a295.6 A ab
6540.7 B a588.3 A abc535.3 B a588.7 A abcd264.2 B a293.6 A abc259.6 B a294.0 A abc
7515.0 B abc594.6 A abc519.0 B a594.5 A abc241.1 B ab291.5 A abc244.5 B a291.4 A abc
8500.8 B abc593.1 A abc503.9 B a594.3 A abc230.3 B ab293.8 A abc233.0 B a294.9 A abc
9482.2 B c558.8 A de484.1 B a562.1 A de220.3 B b265.3 A d222.0 B a268.1 A d
10504.7 B abc552.8 A e503.2 B a553.5 A e242.2 B ab265.8 A d240.9 B a266.4 A d
11513.6 B abc571.9 A bcde514.7 B a574.5 A cde245.8 B ab278.1 A cd246.7 B a280.3 A bcd
12503.0 B abc586.7 A abcd505.2 B a586.4 A abcd237.3 B ab292.2 A abc239.2 B a292.0 A abc
13505.8 B abc568.0 A bcde507.7 B a568.9 A cde242.1 B ab277.4 A cd243.8 B a278.2 A cd
14520.3 B abc582.4 A abcd517.8 B a582.5 A abcd246.2 B ab283.5 A bcd244.0 B a283.6 A bcd
15513.0 B abc610.0 A a513.3 B a605.7 A ab246.6 B ab306.5 A a246.8 B a302.8 A a
16502.0 B abc579.2 A bcde503.2 B a577.3 A cde234.5 B ab281.2 A cd235.5 B a279.6 A bcd
17521.0 B abc576.6 A bcde518.4 B a578.9 A bcde245.9 B ab281.1 A cd243.7 B a283.1 A bcd
18501.3 B abc609.6 A a502.7 B a608.7 A a229.8 B ab302.1 A ab231.0 B a301.3 A a
19512.8 B abc565.9 A cde512.2 B a568.7 A cde245.4 B ab277.3 A cd244.9 B a279.7 A bcd
20536.3 B ab582.5 A abcd532.4 B a582.0 A bcd261.5 B ab289.5 A abc258.1 B a289.1 A abc
Min.482.2552.8484.1553.5220.3265.3222.0266.4
Max.540.7610.0535.3608.7264.2306.5262.2302.8
Range58.557.251.255.243.941.240.236.4
S.E.8.98.87.27.2

Means of DOM (g kg-1) of the 20 tested genotypes for the 2nd sampling date within 1st and 2nd cuts as analysed with and without the covariable (SMI8)Tabelle 11. Mittlere VOM (g kg-1) der 20 untersuchten Sorten für den 2. Erntetermin des 1. und 2. Aufwuchses mit oder ohne Berücksichtigung der Kovariable (SMI8)

Genotype1st Cut2nd Cut
Without SMI8With SMI8Without SMI8With SMI8
1813.2 abc

Means followed by the same letter(s) within the same column are not significantly different according to the LSD test at 0.05 level of probability. SMI8 represents the percentage of tillers above the boot stage.

820.9 a757.0 bcd757.5 bcde
2809.5 abc810.4 a771.9 abc771.6 abcd
3818.9 abc815.3 a752.6 cd752.1 bcde
4844.4 a838.1 a750.2 cd750.2 cde
5801.0 bc812.5 a757.3 abcd758.1 bcde
6788.3 c804.5 a765.8 abcd766.2 abcde
7839.6 ab829.5 a755.5 bcd757.6 bcde
8838.4 ab827.4 a748.7 cd747.0 de
9842.2 ab835.8 a786.0 ab783.2 ab
10813.6 abc818.4 a789.5 a791.1 a
11816.1 abc812.5 a776.3 abc775.1 abcd
12828.1 abc821.2 a768.6 abc768.5 abcde
13831.6 ab827.4 a774.6 abc772.6 abcd
14810.6 abc818.5 a770.2 abc769.4 abcde
15829.1 abc823.0 a735.1 d737.8 e
16830.4 abc823.7 a760.0 abcd760.3 abcde
17809.3 abc815.6 a780.3 abc779.3 abc
18831.8 ab826.5 a734.3 d736.9 e
19814.8 abc815.6 a780.9 abc779.2 abc
20818.9 abc833.0 a759.9 abcd760.9 abcde
Min. DOM content788.3804.5734.3736.9
Max. DOM content844.4838.1789.5791.1
Range56.133.655.254.2
S.E.8.09.16.06.2

Genotypic influence on DOM (g kg-1) in absence and presence of the covariable (SMI8) for the 2nd sampling date within 1st and 2nd cutsTabelle 10. Einfluss der Sorte auf die VOM (g kg-1) mit oder ohne Berücksichtigung der Kovariable (SMI8) für den 2. Erntetermin des 1. und 2. Aufwuchses

Effect1st Cut2nd Cut
Without SMI8With SMI8Without SMI8With SMI8
Genotype3.39***1.216.57***5.45***
Block0.910.691.841.80
Covariable (B)8.60**2.25
Slope-0.70-0.28

F-values of genotype, sampling date, cut and their interactions on yield and feed quality parameters without using covariable (SMI8)Tabelle 6. F-Werte für Sorte, Erntetermin, Aufwuchs und Wechselwirkungen für Ertrag und Futterqualität ohne Kovariable (SMI8)

FactorDry matter yield (t ha-1)NDFADFADLDOM
--------------g kg-1--------------
Genotype (G)1.708.95

Significant at 0.001 level of probability

7.23

Significant at 0.001 level of probability

6.67

Significant at 0.001 level of probability

16.21

Significant at 0.001 level of probability

Cut (C)0.00688.95

Significant at 0.001 level of probability

460.61

Significant at 0.001 level of probability

391.26

Significant at 0.001 level of probability

759.43

Significant at 0.001 level of probability

Sampling date (SD)367.78

Significant at 0.001 level of probability

179.69

Significant at 0.001 level of probability

366.78

Significant at 0.001 level of probability

105.18

Significant at 0.001 level of probability

1144.59

Significant at 0.001 level of probability

Block0.0714.95

Significant at 0.001 level of probability

13.79

Significant at 0.001 level of probability

2.700.84
G × C2.07

Non-significant after applying Bonferroni-Holm test.

2.89

Significant at 0.01 level of probability

2.68

Significant at 0.01 level of probability

3.44

Significant at 0.001 level of probability

5.17

Significant at 0.001 level of probability

G × SD1.480.770.911.131.39
C × SD109.45

Significant at 0.001 level of probability

18.14

Significant at 0.001 level of probability

47.64

Significant at 0.001 level of probability

1.5846.50

Significant at 0.001 level of probability

G × C × SD0.890.981.071.161.59

F-values of genotype, sampling date, cut and their interactions on yield and forage quality parameters with covariable (SMI8)Tabelle 7. F-Werte für Sorte, Erntetermin, Aufwuchs und Wechselwirkungen für Ertrag und Futterqualität mit Kovariable (SMI8)

FactorDry matter yield (t ha-1)NDFADFADLDOM
------------------------g kg-1------------------------
Genotype (G)1.476.78

Significant at 0.001 level of probability.

5.35

Significant at 0.001 level of probability.

4.42

Significant at 0.001 level of probability.

8.78

Significant at 0.001 level of probability.

Cut (C)0.06775.99

Significant at 0.001 level of probability.

526.60

Significant at 0.001 level of probability.

493.86

Significant at 0.001 level of probability.

980.28

Significant at 0.001 level of probability.

Sampling date (SD)122.78

Significant at 0.001 level of probability.

87.94

Significant at 0.001 level of probability.

167.79

Significant at 0.001 level of probability.

44.71

Significant at 0.001 level of probability.

278.84

Significant at 0.001 level of probability.

Block0.1515.94

Significant at 0.001 level of probability.

14.97

Significant at 0.001 level of probability.

2.531.26
Covariable (SMI8)8.64

Significant at 0.01 level of probability.

8.95

Significant at 0.01 level of probability.

15.41

Significant at 0.001 level of probability.

12.27

Significant at 0.01 level of probability.

20.65

Significant at 0.001 level of probability.

G × C1.542.09

Significant at 0.05 level of probability.

2.00

Significant at 0.05 level of probability.

2.19

Significant at 0.05 level of probability.

3.80

Significant at 0.001 level of probability.

G × SD1.350.740.900.971.21
C × SD65.34

Significant at 0.001 level of probability.

7.62

Significant at 0.01 level of probability.

23.69

Significant at 0.001 level of probability.

3.51

Significant at 0.05 level of probability.

12.58

Significant at 0.001 level of probability.

G × C × SD1.031.041.111.151.60
Slope0.900.300.300.05-0.43
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