<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of research is to assess the active substance by a HPLC method for the separation and quantitative determination of loratadine. The method has been developed and validated on the standard solutions, in previous research. The current study was undertaken to present the results obtained from loratadine determination in biological samples (human serum, urine and breast milk). These results may be applicable on patients with different physiological conditions (aging, pregnancy or recently giving birth, etc.) and pathological conditions which may interfere with the metabolism of loratadine. The used HPLC method detected loratadine concentrations in human serum samples, respectively urine samples, at 2 hours after drug administration. The method detected traces of loratadine which passed into breast milk, as well. Data were statistically interpreted using MED CALC 10.2 software. These results show that the applied method can be used for quantitative analysis of loratadine in biological fluids (all permissible limits of quality specifications being in the range 95- 105%).</p></abstract>

The aim of research is to assess the active substance by a HPLC method for the separation and quantitative determination of loratadine. The method has been developed and validated on the standard solutions, in previous research. The current study was undertaken to present the results obtained from loratadine determination in biological samples (human serum, urine and breast milk). These results may be applicable on patients with different physiological conditions (aging, pregnancy or recently giving birth, etc.) and pathological conditions which may interfere with the metabolism of loratadine. The used HPLC method detected loratadine concentrations in human serum samples, respectively urine samples, at 2 hours after drug administration. The method detected traces of loratadine which passed into breast milk, as well. Data were statistically interpreted using MED CALC 10.2 software. These results show that the applied method can be used for quantitative analysis of loratadine in biological fluids (all permissible limits of quality specifications being in the range 95- 105%).

Applications of a DAD-HPLC method for determination of loratadine on biological samples

Faculty of Pharmacy, “Ovidius” University of Constanta, Romania

Faculty of Applied Sciences and Engineering, “Ovidius” University of Constanta, Romania

Ovidius University Annals of Chemistry

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The aim of research is to assess the active substance by a HPLC method for the separation and quantitative determination of loratadine. The method has been...

Applications of a DAD-HPLC method for determination of loratadine on biological samples

Online veröffentlicht: 25. Juni 2015

Seitenbereich: 32 - 36

Eingereicht: 29. Apr. 2015

Akzeptiert: 09. Juni 2015

DOI: https://doi.org/10.1515/auoc-2015-0006

Schlüsselwörter
loratadine, HPLC, biological samples

© Ovidius University Press

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Applications of a DAD-HPLC method for determination of loratadine on biological samples

Online veröffentlicht: 25. Juni 2015

Seitenbereich: 32 - 36

Eingereicht: 29. Apr. 2015

Akzeptiert: 09. Juni 2015

DOI: https://doi.org/10.1515/auoc-2015-0006

Schlüsselwörterloratadine, HPLC, biological samples

© Ovidius University Press

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
loratadine, HPLC, biological samples