1. bookVolume 66 (2017): Issue 1 (March 2017)
Journal Details
License
Format
Journal
eISSN
2544-4646
First Published
04 Mar 1952
Publication timeframe
4 times per year
Languages
English
access type Open Access

Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Published Online: 30 Mar 2017
Volume & Issue: Volume 66 (2017) - Issue 1 (March 2017)
Page range: 107 - 111
Received: 23 Dec 2015
Accepted: 10 May 2016
Journal Details
License
Format
Journal
eISSN
2544-4646
First Published
04 Mar 1952
Publication timeframe
4 times per year
Languages
English
Abstract

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains compared to unexposed controls. AFLP showed a high discriminatory power, confirming the existence of different genotypes within the species and presence of DNA polymorphisms in UVA-exposed colonies.

Keywords

Brunet-Galmés I., A. Busquets, A. Peña, M. Gomila, B. Nogales, E. García-Valdés, J. Lalucat, A. Bennasar and R. Bosch. 2012. Complete genome sequence of the naphthalene-degrading bacterium Pseudomonas stutzeri AN10 (CCUG 29243). J. Bacteriol. 194: 6642–6643.10.1128/JB.01753-12 Search in Google Scholar

Hensgens L.A., A. Tavanti, S. Mogavero, E. Ghelardi and S. Senesi. 2009. AFLP genotyping of Candida metapsilosis clinical isolates: evidence for recombination. Fungal Gen. Biol. 46: 750–758.10.1016/j.fgb.2009.06.006 Search in Google Scholar

Grüntzig V., S.C. Nold, J. Zhou and J.M. Tiedje. 2001. Pseudomonas stutzeri nitrite reductase gene abundance in environmental samples measured by real-time PCR. Appl. Environ. Microbiol. 67: 760–768.10.1128/AEM.67.2.760-768.2001 Search in Google Scholar

Krauss S.L. 2000. Accurate gene diversity estimates from amplified fragment length by polymorphism (AFLP) markers. Mol. Ecol. 9: 1241–1245.10.1046/j.1365-294x.2000.01001.x Search in Google Scholar

Lalucat J., A. Bennasar, R. Bosch, E. García-Valdés and N.J. Palleroni. 2006. Biology of Pseudomonas stutzeri. Microbiol. Mol. Biol. Rev. 70: 510–547.10.1128/MMBR.00047-05 Search in Google Scholar

Lima A.B., L.S. Leão-Vasconcelos, M. Costa de, L.O. Vilefort, M.C. André, M.A. Barbosa and M.A. Prado-Palos. 2015. Pseudomonas spp. isolated from the oral cavity of healthcare workers from an oncology hospital in Midwestern Brazil. Rev Inst Med Trop Sao Paulo. 57: 513–514.10.1590/S0036-46652015000600009 Search in Google Scholar

Santos A.L., V. Oliveira, I. Baptista, I. Henriques, N.C. Gomes, A. Almeida, A. Correia and A. Cunha. 2013. Wavelength dependence of biological damage induced by UV radiation on bacteria. Arch. Microbiol. 195: 63–74.10.1007/s00203-012-0847-5 Search in Google Scholar

Sepúlveda-Torres L.C., J. Zhou, C. Guasp, J. Lalucat, D. Knaebel, J.L. Plank and C. Criddle. 2001. Pseudomonas sp. strain KC represents a new genomovar within Pseudomonas stutzeri. Int. J. Syst. Evol. Microbiol. 51: 2013–2019.10.1099/00207713-51-6-2013 Search in Google Scholar

Sikorski J., J. Lalucat and W. Wackernagel. 2005. Genomovars 11 to 18 of Pseudomonas stutzeri, identified among isolates from soil and marine sediment. Intern. J. Syst. Evol. Microbiol. 55: 1767–1770. Search in Google Scholar

Sikorski J., R. Rosselló-Mora and M.G. Lorenz. 1999. Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis. Syst. Appl. Microbiol. 22: 393–402.10.1016/S0723-2020(99)80048-4 Search in Google Scholar

Tavanti A., L.A. Hensgens, E. Ghelardi, M. Campa and S. Senesi. 2007. Genotyping of Candida orthopsilosis by AFLP reveals genetic diversity among independent isolates and strain maintenance within the same patient. J. Clin. Microbiol. 45: 1455–1462.10.1128/JCM.00243-07186588917329454 Search in Google Scholar

Vos P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman and M. Kuiper. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23: 4407–4414.10.1093/nar/23.21.44073073977501463 Search in Google Scholar

Xiang S.R., M. Cook, S. Saucier, P. Gillespie, R. Socha, R. Scroggins and L.A. Beaudette. 2010. Development of amplified fragment length polymorphism-derived functional strain-specific markers to assess the persistence of 10 bacterial strains in soil microcosms. Appl. Environ. Microbiol. 76: 7126–7135.10.1128/AEM.00574-10297623020817796 Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo