<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium <italic>Pseudomonas stutzeri</italic>, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains compared to unexposed controls. AFLP showed a high discriminatory power, confirming the existence of different genotypes within the species and presence of DNA polymorphisms in UVA-exposed colonies.</p>
</abstract>

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains compared to unexposed controls. AFLP showed a high discriminatory power, confirming the existence of different genotypes within the species and presence of DNA polymorphisms in UVA-exposed colonies.

Use of Amplification Fragment Length Polymorphism to Genotype <italic>Pseudomonas stutzeri</italic> Strains Following Exposure to Ultraviolet Light A

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Use of Amplification Fragment Length Polymorphism to Genotype  Strains Following Exposure to Ultraviolet Light A

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common...

Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Published Online: Mar 30, 2017

Page range: 107 - 111

Received: Dec 23, 2015

Accepted: May 10, 2016

DOI: https://doi.org/10.5604/pjm-2017-107

Keywords
AFLP, polymorphisms, UVA exposure

© 2017 LISA LOMBARDI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Published Online: Mar 30, 2017

Page range: 107 - 111

Received: Dec 23, 2015

Accepted: May 10, 2016

DOI: https://doi.org/10.5604/pjm-2017-107

KeywordsAFLP, polymorphisms, UVA exposure

© 2017 LISA LOMBARDI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
AFLP, polymorphisms, UVA exposure