<abstract xmlns="http://www.w3.org/1999/xhtml">

Alkaline proteases have biotechnological importance due to their activity and stability at alkaline pH. 56 bacteria, capable of growing under alkaline conditions were isolated and their alkaline protease activities were carried out at different parameters to determine their optimum alkaline protease production conditions. Seven isolates were showed higher alkaline protease production capacity than the reference strains. The highest alkaline protease producing isolates (103125 U/g), E114 and C265, were identified as <italic>Bacillus licheniformis</italic> with 99.4% and <italic>Bacillus mojavensis</italic> 99.8% based on 16S rRNA gene sequence similarities, respectively. Interestingly, the isolates identified as <italic>Bacillus safensis</italic> were also found to be high alkaline protease producing strains. Genotypic characterizations of the isolates were also determined by using a wide range of molecular techniques (ARDRA, ITS-PCR, (GTG)5-PCR, BOX-PCR). These different techniques allowed us to differentiate the alkaliphilic isolates and the results were in concurrence with phylogenetic analyses of the 16S rRNA genes. While ITS-PCR provided the highest correlation with 16S rRNA groups, (GTG)5-PCR showed the highest differentiation at species and intra-species level. In this study, each of the biotechnologically valuable alkaline protease producing isolates was grouped into their taxonomic positions with multi-genotypic analyses.
</abstract>

Alkaline proteases have biotechnological importance due to their activity and stability at alkaline pH. 56 bacteria, capable of growing under alkaline conditions were isolated and their alkaline protease activities were carried out at different parameters to determine their optimum alkaline protease production conditions. Seven isolates were showed higher alkaline protease production capacity than the reference strains. The highest alkaline protease producing isolates (103125 U/g), E114 and C265, were identified as Bacillus licheniformis with 99.4% and Bacillus mojavensis 99.8% based on 16S rRNA gene sequence similarities, respectively. Interestingly, the isolates identified as Bacillus safensis were also found to be high alkaline protease producing strains. Genotypic characterizations of the isolates were also determined by using a wide range of molecular techniques (ARDRA, ITS-PCR, (GTG)5-PCR, BOX-PCR). These different techniques allowed us to differentiate the alkaliphilic isolates and the results were in concurrence with phylogenetic analyses of the 16S rRNA genes. While ITS-PCR provided the highest correlation with 16S rRNA groups, (GTG)5-PCR showed the highest differentiation at species and intra-species level. In this study, each of the biotechnologically valuable alkaline protease producing isolates was grouped into their taxonomic positions with multi-genotypic analyses.

A Comparative Study: Taxonomic Grouping of Alkaline Protease Producing Bacilli

Biology Department, Ankara University, Tandogan

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"A Comparative Study: Taxonomic Grouping of Alkaline Protease Producing Bacilli"}

Alkaline proteases have biotechnological importance due to their activity and stability at alkaline pH. 56 bacteria, capable of growing under alkaline...

A Comparative Study: Taxonomic Grouping of Alkaline Protease Producing Bacilli

Published Online: Mar 30, 2017

Page range: 39 - 56

Received: Nov 05, 2015

Accepted: Feb 19, 2016

DOI: https://doi.org/10.5604/pjm-2017-039

Keywords
16S rRNA gene sequence, alkaline protease, alkaliphilic, ARDRA, ITS-PCR, -PCR

© 2017 NILGUN TEKIN et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A Comparative Study: Taxonomic Grouping of Alkaline Protease Producing Bacilli

Published Online: Mar 30, 2017

Page range: 39 - 56

Received: Nov 05, 2015

Accepted: Feb 19, 2016

DOI: https://doi.org/10.5604/pjm-2017-039

Keywords16S rRNA gene sequence, alkaline protease, alkaliphilic, ARDRA, ITS-PCR, -PCR

© 2017 NILGUN TEKIN et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
16S rRNA gene sequence, alkaline protease, alkaliphilic, ARDRA, ITS-PCR, -PCR