1. bookVolume 65 (2016): Issue 4 (December 2016)
Journal Details
License
Format
Journal
eISSN
2544-4646
First Published
04 Mar 1952
Publication timeframe
4 times per year
Languages
English
Open Access

Study of Acid Phosphatase in Solubilization of Inorganic Phosphates by Piriformospora indica

Published Online: 28 Feb 2016
Volume & Issue: Volume 65 (2016) - Issue 4 (December 2016)
Page range: 407 - 412
Received: 24 Nov 2013
Accepted: 23 May 2016
Journal Details
License
Format
Journal
eISSN
2544-4646
First Published
04 Mar 1952
Publication timeframe
4 times per year
Languages
English
Abstract

Phosphorus is an essential plant macronutrient present in the soil. Only a small portion of phosphorus in soil is taken up by plants and the rest of it becomes unavailable to plants as it is immobilized. Phosphate solubilizing microorganisms play a vital role in converting the insoluble form of phosphates to the soluble form. The present paper reports the solubilization of tricalcium phosphate, rock phosphate, single super phosphate, zinc phosphate and aluminum phosphate by Piriformospora indica with the production of organic acids as well as acid phosphatase. The amount of phosphate released (4.73 mg ml–1) and titratable acidity (0.12%) was found to be the highest in the case of single super phosphate as compared to other phosphate sources. High performance liquid chromatography (HPLC) revealed the presence of oxalic acid, lactic acid, citric acid and succinic acid in the media. Highest phosphatase activity was observed with the cell membrane extract of the organism in the presence of zinc phosphate.

Keywords

Agnihotri V.P. 1970. Solubilization of insoluble phosphates by some soil fungi isolated from nursery seedbeds. Can. J. Microbiol. 16(9): 877–880. Search in Google Scholar

Bagyaraj D.J., P.U. Krishnaraj and S.P.S. Khanuja. 2000. Mineral phosphate solubilization: agronomic implications, mechanism and molecular genetics. Proc. Indian Natn. Sci. Acad. (PINSA) 66(2–3): 69–82. Search in Google Scholar

Calleja M., B. Mousain, B. Lecouvreur and J. d’Auzac. 1980. Effects of phosphate deficiency on acid phosphatase activities of three mycorrhizal fungi: Hebeloma edurum Metrod., Suillus granulatus (L. ex Fr.) (in French). Kuntze O. and Pisolithus tinctorius (Pers.) Coker et Couch. Physiol. Vég. 18: 489–504. Search in Google Scholar

Fankem H., D. Nwaga, A. Deubel, L. Dieng, W. Merbach and F.X. Etoa. 2006. Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon. African J. Biotech. 5: 2450–2460. Search in Google Scholar

Fiske C.H. and Y. Subbarow. 1925. The colorimetric determination of phosphorus. J. Biol. Chem. 66: 375–400.10.1016/S0021-9258(18)84756-1 Search in Google Scholar

Gadagi R.S. and T. Sa. 2002. New isolation method for microorganisms solubilizing iron and aluminum phosphates using dyes. Soil Sci. Plant Nutr. 48 (4): 615–618.10.1080/00380768.2002.10409246 Search in Google Scholar

Gyaneshwar P., G.N. Kumar, L.J. Parekh and P.S. Poole. 2002. Role of soil microorganisms in improving P nutrition of plants. Plant Soil. 245: 83–93.10.1023/A:1020663916259 Search in Google Scholar

Fankem H., L. Ngo Nkot, A. Deubel, J. Quinn, W. Merbach, F.-X. Etoa and D. Nwaga. 2008. Solubilization of inorganic phosphates and plant growth promotion by strains of Pseudomonas fluo- rescens isolated from acidic soils of Cameroon. Afr. J. Microbiol. Res. 2: 171–178. Search in Google Scholar

Illmer P. and F. Schinner. 1995. Solubilization of inorganic phosphates by microorganisms isolated from forest soil. Soil Biol. Bio- chem. 24: 389–395.10.1016/0038-0717(92)90199-8 Search in Google Scholar

Jones D.L. 1998. Organic acids in the rhizosphere – a critical review. Plant Soil. 205: 25–44.10.1023/A:1004356007312 Search in Google Scholar

Kang S.C., C.G. Ha, T.G. Lee and D.K. Maheshwari. 2002. Solubilization of insoluble inorganic phosphates by a soil-inhabiting fungus Fomitopsis sp. PS 102. Curr. Sci. 82: 439–442. Search in Google Scholar

Khan A.A., G. Jilani, M.S. Akhtar, S.M. Saqlan Naqvi and M. Rasheed. 2009. Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J. Agric. Biol. Sci. 1(1): 48–58. Search in Google Scholar

Kraiker H.P. and R.E Burch. 1973. Thin layer chromatography of Krebs cycle acids. Z. Klin. Chem. Klin. Biochem. 11: 393–39710.1515/cclm.1973.11.9.393Search in Google Scholar

Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259): 680–685.10.1038/227680a0 Search in Google Scholar

Lowry O.H., ?.J. Rosebrough, A.L. Farr and R.J. Randall. 1951. Protein measurement by Folin phenol reagent. J. Biol. Chem. 193: 265–275.10.1016/S0021-9258(19)52451-6 Search in Google Scholar

Malla R., R. Prasad, R. Kumari, P.H. Giang, U. Pokharel, R. Oelmüller and A. Varma. 2004. Phosphorus solubilizing symbiotic fungus: Piriformospora indica. Endocytobiosis Cell Res. 15(2): 579–600. Search in Google Scholar

Mardad I., A. Serrano and A. Soukri. 2013. Solubilization of inorganic phosphate and production of organic acids by bacteria isolatedfrom a Moroccan mineral phosphate deposit. Afr. J. Microbiol. Res. 7(8): 626–635. Search in Google Scholar

Nath R., G.D. Sharma and M. Barooah. 2012. Efficiency of tricalcium phosphate solubilization by two different endophytic Penicil- lium sp. isolated from tea (Camellia sinensis L.). Euro. J. Exp. Bio. 2(4): 1354–1358. Search in Google Scholar

Nenwani V., P. Doshi, T. Saha and S. Rajkumar. 2010. Isolation and characterization of a fungal isolate for phosphate solubilization and plant growth promoting activity. J. Yeast Fungal Res. 1: 9–14. Search in Google Scholar

Omar S.A. 1998. The role of rock-phosphate-solubilizing fungi and vesicular-arbuscular mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate. World J. Microbiol. Biotechnol. 14: 211–218. Search in Google Scholar

Panhwar Q.A., R. Othman, A.R. Zaharah, S. Meon and M.R. Ismail. 2011. Effect of phosphatic fertilizer on root colonization of aerobic rice by phosphate-solubilizing bacteria. Abstract of International Conference on Food Engineering and Biotechnology. IACSIT Press, Singapoore. Search in Google Scholar

Reyes I., L. Bernier, R.R. Simard and H. Antoun. 1999. Effect of nitrogen source on the solubilization of different inorganic phosphates by an isolate of Penicillium rugulosum and two UV-induced mutants. FEMS Microbiology Ecology. 28: 281–290.10.1111/j.1574-6941.1999.tb00583.x Search in Google Scholar

Rodriguez H. and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotech Adv. 17: 319–339.10.1016/S0734-9750(99)00014-2 Search in Google Scholar

Ryan P.R., E. Delhaize and D.L. Jones. 2001. Function and mechanism of organic anion exudation from plant roots. Annl. Rev. Plant Physiol. Plant Mol. Biol. 52: 527–560.10.1146/annurev.arplant.52.1.527 Search in Google Scholar

Sagervanshi A., P. Kumari, A. Nagee and A. Kumar. 2012. Media optimization for inorganic phosphate solubilizing bacteria isolated from Anand argiculture soil. Int. J. Lif. Sci. Pharm. Res. 2(3): 245–255.Search in Google Scholar

Singh A., J. Sharma, K.-H. Rexer and A. Varma. 2000. Plant productivity determinants beyond minerals, water and light: Pirifor- mospora indica – A revolutionary plant growth promoting fungus. Curr. Sci. 79(11): 1548–1554. Search in Google Scholar

Tallapragada P. and U. Seshachala. 2012. Phosphate-solubilizing microbes and their occurrence in the rhizospheres of Piper betel in Karnataka, India. Turk. J. Biol. 36:25–35.10.3906/biy-1012-160 Search in Google Scholar

Vazquez P., G. Holguin, M.E. Puente, A. Lopez-Cortes and Y. Bashan. 2000. Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biol. Fertil. Soils. 30: 460–468.10.1007/s003740050024 Search in Google Scholar

Vyas P. and A. Gulati. 2009. Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonas. BMC Microbio logy 9: 174–189.10.1186/1471-2180-9-174 Search in Google Scholar

Whitelaw M.A. 2000. Growth promotion of plants inoculated with phosphate-solubilizing fungi. Adv. Agron. 69: 99–151.10.1016/S0065-2113(08)60948-7 Search in Google Scholar

Xiao C., R. Chi, X. Pan, F. Liu and J. He. 2013. Rock phosphate solubilization by four yeast strains. Ann. Microbial. 63(1): 173–178. Search in Google Scholar

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