<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The microbiology of caves is an important topic for better understanding subsurface biosphere diversity. The diversity and taxonomic composition of bacterial communities associated with cave walls of the Oylat Cave was studied first time by molecular cloning based on Sanger/pyrosequencing approach. Results showed an average of 1,822 operational taxonomic units per sample. Clones analyzed from Oylat Cave were found to belong to 10 common phyla within the domain Bacteria. <italic>Proteobacteria</italic> dominated the phyla, followed by <italic>Actinobacteria, Acidobacteria</italic> and <italic>Nitrospirae</italic>. Shannon diversity index was between to 3.76 and 5.35. The robust analysis conducted for this study demonstrated high bacterial diversity on cave rock wall surfaces.</p>
</abstract>

The microbiology of caves is an important topic for better understanding subsurface biosphere diversity. The diversity and taxonomic composition of bacterial communities associated with cave walls of the Oylat Cave was studied first time by molecular cloning based on Sanger/pyrosequencing approach. Results showed an average of 1,822 operational taxonomic units per sample. Clones analyzed from Oylat Cave were found to belong to 10 common phyla within the domain Bacteria. Proteobacteria dominated the phyla, followed by Actinobacteria, Acidobacteria and Nitrospirae. Shannon diversity index was between to 3.76 and 5.35. The robust analysis conducted for this study demonstrated high bacterial diversity on cave rock wall surfaces.

Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The microbiology of caves is an important topic for better understanding subsurface biosphere diversity. The diversity and taxonomic composition of bacterial...

Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach

Published Online: Mar 15, 2016

Page range: 69 - 75

Received: Nov 03, 2015

Accepted: Apr 19, 2015

DOI: https://doi.org/10.5604/pjm-2016-069

Keywords
bacterial diversity, Oylat Cave, Sanger/pyrosequencing, subsurface biosphere

© 2016 YASEMÌN GULECAL-PEKTAS published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach

Published Online: Mar 15, 2016

Page range: 69 - 75

Received: Nov 03, 2015

Accepted: Apr 19, 2015

DOI: https://doi.org/10.5604/pjm-2016-069

Keywordsbacterial diversity, Oylat Cave, Sanger/pyrosequencing, subsurface biosphere

© 2016 YASEMÌN GULECAL-PEKTAS published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
bacterial diversity, Oylat Cave, Sanger/pyrosequencing, subsurface biosphere