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<p>The capability of the biosurfactant-producing strain <italic>Rhodococcus wratislawiensis</italic> BN38 to mineralize both aromatic and aliphatic xeno- biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by <italic>Rhodococcus</italic> free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l n-hexadecane (40 cycles). The alkanotrophic strain <italic>R. wratislawiensis</italic> BN38 preferably degraded hexadecane rather than phenol. SEM revealed well preserved cells entrapped in the heterogeneous super-macroporous structure of the cryogel which allowed unhindered mass transfer of xenobiotics. The immobilized strain can be used in real conditions for the treatment of contaminated industrial waste water.
</p>
</abstract>

The capability of the biosurfactant-producing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno- biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by Rhodococcus free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l n-hexadecane (40 cycles). The alkanotrophic strain R. wratislawiensis BN38 preferably degraded hexadecane rather than phenol. SEM revealed well preserved cells entrapped in the heterogeneous super-macroporous structure of the cryogel which allowed unhindered mass transfer of xenobiotics. The immobilized strain can be used in real conditions for the treatment of contaminated industrial waste water.

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain <italic>Rhodococcus wratislawiensis</italic> BN38

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain  BN38

The capability of the biosurfactant-producing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno- biotics was proved....

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Published Online: Aug 26, 2016

Page range: 287 - 293

Received: Oct 20, 2014

Accepted: Feb 11, 2016

DOI: https://doi.org/10.5604/17331331.1215608

Keywords
sp., biodegradation, immobilization, n-hexadecane, phenol

© 2016 ALEXANDER E. HRISTOV et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Published Online: Aug 26, 2016

Page range: 287 - 293

Received: Oct 20, 2014

Accepted: Feb 11, 2016

DOI: https://doi.org/10.5604/17331331.1215608

Keywordssp., biodegradation, immobilization, n-hexadecane, phenol

© 2016 ALEXANDER E. HRISTOV et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
sp., biodegradation, immobilization, n-hexadecane, phenol