<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.1103.555_s_004_w2aab3b7b5b1b6b1aab1c17b1Aa"><h3>Background</h3><p>Bacillus Calmette–Guérin (BCG) Tokyo 172-1 strain has been used as a parent strain since 1988 by Queen Saovabha Memorial Institute, Thai Red Cross Society to manufacture BCG vaccine in Thailand. In general, the BCG Tokyo strain includes 2 BCG type populations: types I and II. Several studies have found that the type I population is higher than type II. Polymerase chain reaction has shown the presence of 2 types of subpopulations in the BCG Thai Red Cross Society (TRCS) strain. However, the characteristics of all genes from BCG TRCS have not been studied in depth, including whether or not the original features of the BCG Tokyo 172-1 strain are still retained.</p></sec><sec id="j_1905-7415.1103.555_s_005_w2aab3b7b5b1b6b1aab1c17b2Aa"><h3>Objectives</h3><p>To compare BCG TRCS and BCG Tokyo 172-1 for differences between their genomes.</p></sec><sec id="j_1905-7415.1103.555_s_006_w2aab3b7b5b1b6b1aab1c17b3Aa"><h3>Methods</h3><p>The whole genome of BCG TRCS was analyzed by next-generation sequencing.</p></sec><sec id="j_1905-7415.1103.555_s_007_w2aab3b7b5b1b6b1aab1c17b4Aa"><h3>Results</h3><p>BCG TRCS contained 23 different points and was 4 bases less in length than BCG Tokyo 172-1. However, its main features remain similar to those of the original BCG Tokyo 172-1 strain.</p></sec><sec id="j_1905-7415.1103.555_s_008_w2aab3b7b5b1b6b1aab1c17b5Aa"><h3>Conclusions</h3><p>The changes in the genome may be caused by a variety of factors including the strain of Tokyo 172, vaccine processing, storage, and natural changes in the genome.</p></sec></abstract>

BackgroundBacillus Calmette–Guérin (BCG) Tokyo 172-1 strain has been used as a parent strain since 1988 by Queen Saovabha Memorial Institute, Thai Red Cross Society to manufacture BCG vaccine in Thailand. In general, the BCG Tokyo strain includes 2 BCG type populations: types I and II. Several studies have found that the type I population is higher than type II. Polymerase chain reaction has shown the presence of 2 types of subpopulations in the BCG Thai Red Cross Society (TRCS) strain. However, the characteristics of all genes from BCG TRCS have not been studied in depth, including whether or not the original features of the BCG Tokyo 172-1 strain are still retained.ObjectivesTo compare BCG TRCS and BCG Tokyo 172-1 for differences between their genomes.MethodsThe whole genome of BCG TRCS was analyzed by next-generation sequencing.ResultsBCG TRCS contained 23 different points and was 4 bases less in length than BCG Tokyo 172-1. However, its main features remain similar to those of the original BCG Tokyo 172-1 strain.ConclusionsThe changes in the genome may be caused by a variety of factors including the strain of Tokyo 172, vaccine processing, storage, and natural changes in the genome.

Genetic characteristics of <italic>Mycobacterium bovis</italic> BCG Thai Red Cross Society strain

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic characteristics of  BCG Thai Red Cross Society strain

BackgroundBacillus Calmette–Guérin (BCG) Tokyo 172-1 strain has been used as a parent strain since 1988 by Queen Saovabha Memorial Institute, Thai Red Cross...

Genetic characteristics of Mycobacterium bovis BCG Thai Red Cross Society strain

Article Category: Brief communication (Original)

Published Online: Aug 31, 2017

Page range: 245 - 254

DOI: https://doi.org/10.5372/1905-7415.1103.555

Keywords
BCG, BCG Tokyo, next-generation sequencing

© 2017 Wichit Thaveekarn, Sunchai Payungporn, Narumol Pakmanee, Sunutcha Suntrarachun, Suchitra Khunsap, Suthidee Petsong

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic characteristics of Mycobacterium bovis BCG Thai Red Cross Society strain

Article Category: Brief communication (Original)

Published Online: Aug 31, 2017

Page range: 245 - 254

DOI: https://doi.org/10.5372/1905-7415.1103.555

KeywordsBCG, BCG Tokyo, next-generation sequencing

© 2017 Wichit Thaveekarn, Sunchai Payungporn, Narumol Pakmanee, Sunutcha Suntrarachun, Suchitra Khunsap, Suthidee Petsong

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
BCG, BCG Tokyo, next-generation sequencing