<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Norovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide, causing a substantial disease burden. Recent years have witnessed a dramatic increase in norovirus outbreaks in China, significantly higher than in previous years, among which GII norovirus is the predominant prevalent strain. Therefore, rapid norovirus diagnosis is critical for clinical treatment and transmission control. Hence, we developed a molecular assay based on RPA combined with the CRISPER-CAS12a technique targeting the conserved region of the GII norovirus genome, the results of which could be displayed by fluorescence curves and immunochromatographic lateral-flow test strips. The reaction only required approximately 50 min, and the results were visible by the naked eye with a sensitivity reaching 10<sup>2</sup> copies/μl. Also, our method does not cross-react with other common pathogens that cause intestinal diarrhea. Furthermore, this assay was easy to perform and inexpensive, which could be widely applied for detecting norovirus in settings including medical institutions at all levels, particularly township health centers in low-resource areas.</p>
</abstract>

Norovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide, causing a substantial disease burden. Recent years have witnessed a dramatic increase in norovirus outbreaks in China, significantly higher than in previous years, among which GII norovirus is the predominant prevalent strain. Therefore, rapid norovirus diagnosis is critical for clinical treatment and transmission control. Hence, we developed a molecular assay based on RPA combined with the CRISPER-CAS12a technique targeting the conserved region of the GII norovirus genome, the results of which could be displayed by fluorescence curves and immunochromatographic lateral-flow test strips. The reaction only required approximately 50 min, and the results were visible by the naked eye with a sensitivity reaching 102 copies/μl. Also, our method does not cross-react with other common pathogens that cause intestinal diarrhea. Furthermore, this assay was easy to perform and inexpensive, which could be widely applied for detecting norovirus in settings including medical institutions at all levels, particularly township health centers in low-resource areas.

Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Norovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide,...

Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a

Article Category: ORIGINAL PAPER

Published Online: Mar 04, 2024

Page range: 89 - 97

Received: Oct 28, 2023

Accepted: Jan 22, 2024

DOI: https://doi.org/10.33073/pjm-2024-009

Keywords
GII norovirus, gastroenteritis, RPA, CRISPR-Cas12a, diagnostic

© 2024 Xinyi Hu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a

Article Category: ORIGINAL PAPER

Published Online: Mar 04, 2024

Page range: 89 - 97

Received: Oct 28, 2023

Accepted: Jan 22, 2024

DOI: https://doi.org/10.33073/pjm-2024-009

KeywordsGII norovirus, gastroenteritis, RPA, CRISPR-Cas12a, diagnostic

© 2024 Xinyi Hu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
GII norovirus, gastroenteritis, RPA, CRISPR-Cas12a, diagnostic