<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Lignin recalcitrance is a key issue in producing value-added products from lignocellulose biomass. <italic>In situ</italic> biodegradable lignin-modifying enzymes-producing bacteria are considered a suitable solution to lignin biodegradation problems, but exploitation of ligninolytic bacteria is still limited to date. Hence, this study aimed to isolate and characterize potential lignin peroxidase ligninolytic bacteria from decomposing soil, sawdust, and cow dung at Richard Bay, South Africa. The samples were collected and cultured in the lignin-enriched medium. Pure isolated colonies were characterized through 16S rRNA gene sequencing. The ability of the isolates to grow and utilize aromatic monomers (veratryl and guaiacol alcohol) and decolorize lignin-like dyes (Azure B, Congo Red, Remazol Brilliant Blue R) was evaluated. Of the twenty-six (26) bacteria isolates 10 isolates, including <italic>Pseudomonas</italic> spp. (88%), <italic>Enterobacter</italic> spp. (8%), and <italic>Escherichia coli</italic> (4%) were identified as true lignin peroxidase producers. <italic>Pseudomonas aeruginosa (</italic>CP031449.2) and <italic>E. coli</italic> (LR025096.1) exhibited the highest ligninolytic activities. These isolates could potentially be exploited in the industry and wastewater treatment as effective lignin degrading agents.</p>
</abstract>

Lignin recalcitrance is a key issue in producing value-added products from lignocellulose biomass. In situ biodegradable lignin-modifying enzymes-producing bacteria are considered a suitable solution to lignin biodegradation problems, but exploitation of ligninolytic bacteria is still limited to date. Hence, this study aimed to isolate and characterize potential lignin peroxidase ligninolytic bacteria from decomposing soil, sawdust, and cow dung at Richard Bay, South Africa. The samples were collected and cultured in the lignin-enriched medium. Pure isolated colonies were characterized through 16S rRNA gene sequencing. The ability of the isolates to grow and utilize aromatic monomers (veratryl and guaiacol alcohol) and decolorize lignin-like dyes (Azure B, Congo Red, Remazol Brilliant Blue R) was evaluated. Of the twenty-six (26) bacteria isolates 10 isolates, including Pseudomonas spp. (88%), Enterobacter spp. (8%), and Escherichia coli (4%) were identified as true lignin peroxidase producers. Pseudomonas aeruginosa (CP031449.2) and E. coli (LR025096.1) exhibited the highest ligninolytic activities. These isolates could potentially be exploited in the industry and wastewater treatment as effective lignin degrading agents.

Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa)

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Lignin recalcitrance is a key issue in producing value-added products from lignocellulose biomass. In situ biodegradable lignin-modifying enzymes-producing...

Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa)

Article Category: ORIGINAL PAPER

Published Online: Jun 14, 2023

Page range: 117 - 124

Received: Nov 07, 2022

Accepted: Feb 27, 2023

DOI: https://doi.org/10.33073/pjm-2023-003

Keywords
lignin, bacteria, ligninolytic activity, lignin peroxidase, 16S rDNA

© 2023 Sindiswa L. Dube et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa)

Article Category: ORIGINAL PAPER

Published Online: Jun 14, 2023

Page range: 117 - 124

Received: Nov 07, 2022

Accepted: Feb 27, 2023

DOI: https://doi.org/10.33073/pjm-2023-003

Keywordslignin, bacteria, ligninolytic activity, lignin peroxidase, 16S rDNA

© 2023 Sindiswa L. Dube et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
lignin, bacteria, ligninolytic activity, lignin peroxidase, 16S rDNA