<abstract xmlns="http://www.w3.org/1999/xhtml">

<italic>Staphylococcus aureus</italic> is an important causative pathogen of bloodstream infections. An amplification assay such as real-time PCR is a sensitive, specific technique to detect <italic>S. aureus</italic>. However, it needs well-trained personnel, and costs are high. A literature review focusing on rapid and simple methods for diagnosing <italic>S. aureus</italic> was performed. The following methods were included: (a) Hybrisep <italic>in situ</italic> hybridization test, (b) T2Dx system, (c) BinaxNow <italic>Staphylococcus aureus</italic> and PBP2a, (d) Gram staining, (e) PNA FISH and QuickFISH, (f) Accelerate PhenoTM system, (g) MALDI-TOF MS, (h) BioFire FilmArray, (i) Xpert MRSA/SA. These rapid and simple methods can rapidly identify <italic>S. aureus</italic> in positive blood cultures or direct blood samples. Furthermore, BioFire FilmArray and Xpert MRSA/SA identify methicillin-resistant <italic>S. aureus</italic> (MRSA), and the Accelerate PhenoTM system can also provide antimicrobial susceptibility testing (AST) results. The rapidity and simplicity of results generated by these methods have the potential to improve patient outcomes and aid in the prevention of the emergence and transmission of MRSA.
<inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2022-050_eq_003.png"></inline-graphic></abstract>

Staphylococcus aureus is an important causative pathogen of bloodstream infections. An amplification assay such as real-time PCR is a sensitive, specific technique to detect S. aureus. However, it needs well-trained personnel, and costs are high. A literature review focusing on rapid and simple methods for diagnosing S. aureus was performed. The following methods were included: (a) Hybrisep in situ hybridization test, (b) T2Dx system, (c) BinaxNow Staphylococcus aureus and PBP2a, (d) Gram staining, (e) PNA FISH and QuickFISH, (f) Accelerate PhenoTM system, (g) MALDI-TOF MS, (h) BioFire FilmArray, (i) Xpert MRSA/SA. These rapid and simple methods can rapidly identify S. aureus in positive blood cultures or direct blood samples. Furthermore, BioFire FilmArray and Xpert MRSA/SA identify methicillin-resistant S. aureus (MRSA), and the Accelerate PhenoTM system can also provide antimicrobial susceptibility testing (AST) results. The rapidity and simplicity of results generated by these methods have the potential to improve patient outcomes and aid in the prevention of the emergence and transmission of MRSA.

Rapid and Simple Approaches for Diagnosis of <italic>Staphylococcus aureus</italic> in Bloodstream Infections

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Rapid and Simple Approaches for Diagnosis of  in Bloodstream Infections

Staphylococcus aureus is an important causative pathogen of bloodstream infections. An amplification assay such as real-time PCR is a sensitive, specific...

Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections

Article Category: Minireview

Published Online: Dec 09, 2022

Page range: 481 - 489

Received: Aug 29, 2022

Accepted: Nov 01, 2022

DOI: https://doi.org/10.33073/pjm-2022-050

Keywords
bloodstream infections, rapid diagnostics, antimicrobial stewardship, bacteremia

© 2022 Rui Duan and Pei Wang published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections

Article Category: Minireview

Published Online: Dec 09, 2022

Page range: 481 - 489

Received: Aug 29, 2022

Accepted: Nov 01, 2022

DOI: https://doi.org/10.33073/pjm-2022-050

Keywordsbloodstream infections, rapid diagnostics, antimicrobial stewardship, bacteremia

© 2022 Rui Duan and Pei Wang published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
bloodstream infections, rapid diagnostics, antimicrobial stewardship, bacteremia