<abstract xmlns="http://www.w3.org/1999/xhtml">Daptomycin is a cyclolipopeptide antibiotic produced by <italic>Streptomyces roseosporus</italic>. It is widely used to treat drug-resistant bacterial infections; however, daptomycin yield in wild strains is very low. To improve the daptomycin production by the strain BNCC 342432, a modified method of ribosome engineering with superposition of streptomycin resistance was adopted in this study. The highest-yield mutant strain SR-2620 was obtained by increasing streptomycin resistance of BNCC 342432, and achieved daptomycin production of 38.5 mg/l in shake-flask fermentation, 1.79-fold higher than the parent strain and its heredity stability was stable. The morphological characteristics of the two strains were significantly different, and the 440th base G of the <italic>rpsL</italic> gene in the mutant strain was deleted, which resulted in a frameshift mutation. Our results demonstrate that gradually increasing strain resistance to streptomycin was an effective breeding method to improve daptomycin yield in <italic>S. roseosporus</italic>.<fig id="j_pjm-2022-041_fig_001a" position="float" fig-type="figure">
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2022-041_fig_001a.jpg"></graphic>
</fig></abstract>

Daptomycin is a cyclolipopeptide antibiotic produced by Streptomyces roseosporus. It is widely used to treat drug-resistant bacterial infections; however, daptomycin yield in wild strains is very low. To improve the daptomycin production by the strain BNCC 342432, a modified method of ribosome engineering with superposition of streptomycin resistance was adopted in this study. The highest-yield mutant strain SR-2620 was obtained by increasing streptomycin resistance of BNCC 342432, and achieved daptomycin production of 38.5 mg/l in shake-flask fermentation, 1.79-fold higher than the parent strain and its heredity stability was stable. The morphological characteristics of the two strains were significantly different, and the 440th base G of the rpsL gene in the mutant strain was deleted, which resulted in a frameshift mutation. Our results demonstrate that gradually increasing strain resistance to streptomycin was an effective breeding method to improve daptomycin yield in S. roseosporus.

Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Daptomycin is a cyclolipopeptide antibiotic produced by Streptomyces roseosporus. It is widely used to treat drug-resistant bacterial infections; however,...

Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition

Article Category: Original Paper

Published Online: Sep 24, 2022

Page range: 463 - 471

Received: May 05, 2022

Accepted: Aug 02, 2022

DOI: https://doi.org/10.33073/pjm-2022-041

Keywords
daptomycin, streptomycin resistance, strain breeding, fermentation,

© 2022 Shuaibei Chu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition

Article Category: Original Paper

Published Online: Sep 24, 2022

Page range: 463 - 471

Received: May 05, 2022

Accepted: Aug 02, 2022

DOI: https://doi.org/10.33073/pjm-2022-041

Keywordsdaptomycin, streptomycin resistance, strain breeding, fermentation,

© 2022 Shuaibei Chu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
daptomycin, streptomycin resistance, strain breeding, fermentation,