Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants

GOKHAN DOGAN; BILGIN TASKIN

Open Access

Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants

GOKHAN DOGAN

and

BILGIN TASKIN

| Sep 17, 2021

Polish Journal of Microbiology

Volume 70 (2021): Issue 3 (September 2021)

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Published Online: Sep 17, 2021

Page range: 297 - 304

Received: Mar 24, 2021

Accepted: Jun 07, 2021

DOI: https://doi.org/10.33073/pjm-2021-026

Keywords
endophytic bacteria, extracellular enzymes, 16S rRNA gene, Poaceae family, Illumina MiSeq

© 2021 Gokhan Dogan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Petri dishes with colonies surrounded by zones of extracellular enzymatic activity; a) protease, b) amylase, c) lipase, d) cellulase, e) pectinase, f) xylanase.

Relative frequency (%) of strains (from a total of 128) producing individual hydrolytic enzymes.

The phylogenetic tree constructed by the maximum likelihood method and rooted by midpoint-rooting. Branches are scaled in terms of the expected number of nucleotide substitutions per site. Numbers at nodes indicate the bootstrap support values larger than 60% from maximum likelihood (left) and maximum parsimony (right) bootstrapping. GenBank accession numbers are given in brackets.

Identification of strains according to the results of sequence analysis using the EzBioCloud database and GenBank accession numbers.

Code of the isolates	Top-hit reference species	Top-hit reference strain	Similarity(%)	Coverage(%)	GenBankAccession Numbers
G119Y1T	Bacillus toyonensis	BCT-7112	100.00	70.10	MW752891
G118S2T	Pseudomonas congelans	DSM 14939	100.00	100.0	MW752990
G117Y1T	Streptococcus thermophilus	ATCC 19258	94.58	89.30	MW774413
G116K1T	Micrococcus luteus	NCTC 2665	99.58	100.00	MW755305
G113Y3	Bacillus halotolerans	ATCC 25096	99.93	100.00	MW753050
G107Y2	Curtobacterium flaccumfaciens	LMG 3645	100.00	100.00	MW753051
G105Y1	Bacillus subtilis subsp. inaquosorum	KCTC 13429	99.92	84.50	MW753052
G105S1	Bacillus idriensis	SMC 4352-2	99.58	100.00	MW753132
G100Y1	Paenibacillus nuruki	TI45-13ar	99.25	100.00	MW753131
G90Y2	Paenibacillus tundrae	A10b	99.84	83.30	MW753134
G90S1	Curtobacterium flaccumfaciens	LMG 3645	100.00	100.00	MW757038
G88K1	Pseudomonas orientalis	CFML 96-170	99.62	89.20	MW753212
G83S3	Paenibacillus seodonensis	DCT19	99.23	88.00	MW753225
G80K3	Paenibacillus xylanexedens	B22a	99.80	100.00	MW753226
G70K2	Paenibacillus xylanexedens	B22a	99.80	100.00	MW753223
G42K2	Chryseobacterium luteum	DSM 18605	99.44	100.00	MW753224

The plant species and the tissues from which the endophytic bacteria were isolated and enzymatic indexes (EIs) of hydrolytic enzymes of 16 strains selected for the 16S rRNA gene amplicon sequence analysis.

Isolate No	Host Plant	Plant Tissue	Protease	Lipase	Amylase	Cellulase	Pectinase	Xylanase
G90Y2	Aegilops sp.	Leaf	3.46 ± 0.15^efg	9.80 ± 0.20^a	2.14 ± 0.03^de	6.10 ± 0.16^cd	1.73 ± 0.03^c	–
G90S1	Aegilops sp.	Stem	2.94 ± 0.08^gh1	6.79 ± 2.01^bc	3.23 ± 0.09^bc	5.02 ± 0.27^de	–	–
G88K1	Triticum aestivum L.	Root	3.78 ± 0.06^def	1.90 ± 0.11^e	–	–	–	2.88 ± 0.38^ns
G83S3	Triticum aestivum L.	Stem	2.85 ± 0.05^h1	3.67 ± 0.15^de	3.91 ± 0.37^ab	4.40 ± 0.10^e	2.05 ± 0.05^bc	–
G105Y1	Dactylis glomerata L.	Leaf	7.29 ± 0.71^a	1.87 ± 0.34^e	3.03 ± 0.29^bcd	12.75 ± 1.38^a	3.81 ± 0.38^a	–
G105S1	Dactylis spp.	Stem	–	–	–	–	–	–
G100Y1	Festuca spp.	Leaf	3.40 ± 0.12^fgh	6.96 ± 0.54^b	2.18 ± 0.08^de	7.02 ± 0.46^c	–	–
G80K3	Secale cereale L.	Root	4.03 ± 0.17^de	–	3.05 ± 0.13^bcd	–	4.44 ± 0.90^a	–
G70K2	Secale cereale L.	Root	2.73 ± 0.34¹	7.24 ± 0.78^b	2.69 ± 0.04^cd	4.07 ± 0.13^ef	2.34 ± 0.18^bc	–
G42K2	Cultivated Poaceae spp.	Root	3.57 ± 0.20^ef	4.89 ± 0.22^bcd	2.68 ± 0.09^cd	2.66 ± 0.04^f	1.76 ± 0.14^c	–
G119Y1T	Eremopoa sp.	Leaf	–	4.37 ± 0.15^cd	1.29 ± 0.04^e	7.50 ± 0.00^c	–	–
G118S2T	Eremopoa songarica L.	Stem	4.22 ± 0.16^cd	4.46 ± 0.22^cd	1.69 ± 0.08^de	3.46 ± 0.19^ef	–	2.65 ± 0.41^ns
G117Y1T	Eremopoa sp.	Leaf	3.22 ± 0.13^fgh1	6.32 ± 1.78^bc	2.81 ± 0.01^cd	2.46 ± 0.12^f	–	1.90 ± 0.27^ns
G116K1T	Eremopoa songarica L.	Root	4.68 ± 0.25^bc	1.91 ± 0.18^e	–	–	–	–
G113Y3	Triticum aestivum L.	Leaf	5.12 ± 0.07^b	3.15 ± 0.13^de	4.70 ± 0.17^a	9.77 ± 0.42^b	3.48 ± 0.29^ab	1.75 ± 0.25^ns
G107Y2	Triticum aestivum L.	Leaf	3.26 ± 0.09^fgh1	7.33 ± 0.67^b	2.68 ± 0.27^cd	4.95 ± 0.30^de	–	–

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Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants

Article Category: original-paper

Published Online: Sep 17, 2021

Page range: 297 - 304

Received: Mar 24, 2021

Accepted: Jun 07, 2021

DOI: https://doi.org/10.33073/pjm-2021-026

Keywords
endophytic bacteria, extracellular enzymes, 16S rRNA gene, Poaceae family, Illumina MiSeq

© 2021 Gokhan Dogan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Identification of strains according to the results of sequence analysis using the EzBioCloud database and GenBank accession numbers.

The plant species and the tissues from which the endophytic bacteria were isolated and enzymatic indexes (EIs) of hydrolytic enzymes of 16 strains selected for the 16S rRNA gene amplicon sequence analysis.

Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants

Article Category: original-paper

Published Online: Sep 17, 2021

Page range: 297 - 304

Received: Mar 24, 2021

Accepted: Jun 07, 2021

DOI: https://doi.org/10.33073/pjm-2021-026

Keywordsendophytic bacteria, extracellular enzymes, 16S rRNA gene, Poaceae family, Illumina MiSeq

© 2021 Gokhan Dogan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Identification of strains according to the results of sequence analysis using the EzBioCloud database and GenBank accession numbers.

The plant species and the tissues from which the endophytic bacteria were isolated and enzymatic indexes (EIs) of hydrolytic enzymes of 16 strains selected for the 16S rRNA gene amplicon sequence analysis.

Keywords
endophytic bacteria, extracellular enzymes, 16S rRNA gene, Poaceae family, Illumina MiSeq