<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of disease. In this study, the ability of 128 endophyte bacterial isolates from some cultivated and wild grain plants (Poaceae family) in Van, Turkey, were investigated in terms of producing several extracellular hydrolytic enzymes. It was demonstrated that lipases, proteases, amylases, cellulases, pectinases, and xylanases were produced by the bacteria with relative frequencies of 74.2%, 65.6%, 55.4%, 32%, 21.8%, and 7.8%, respectively. In addition, molecular identification of a certain number of isolates selected according to their enzyme-producing capabilities was performed by 16S rRNA gene sequencing using a next-generation sequencing platform. As a result of the analysis, the isolates yielded certain strains belonging to <italic>Pseudomonas, Micrococcus, Paenibacillus, Streptococcus, Curtobacterium, Chryseobacterium</italic>, and <italic>Bacillus</italic> genera. Also, the strain G117Y1T was evaluated as a member of potential novel species based on 16S rRNA sequencing results.</p></abstract>

Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of disease. In this study, the ability of 128 endophyte bacterial isolates from some cultivated and wild grain plants (Poaceae family) in Van, Turkey, were investigated in terms of producing several extracellular hydrolytic enzymes. It was demonstrated that lipases, proteases, amylases, cellulases, pectinases, and xylanases were produced by the bacteria with relative frequencies of 74.2%, 65.6%, 55.4%, 32%, 21.8%, and 7.8%, respectively. In addition, molecular identification of a certain number of isolates selected according to their enzyme-producing capabilities was performed by 16S rRNA gene sequencing using a next-generation sequencing platform. As a result of the analysis, the isolates yielded certain strains belonging to Pseudomonas, Micrococcus, Paenibacillus, Streptococcus, Curtobacterium, Chryseobacterium, and Bacillus genera. Also, the strain G117Y1T was evaluated as a member of potential novel species based on 16S rRNA sequencing results.

Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants"}

Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of...

Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants

Article Category: original-paper

Published Online: Sep 17, 2021

Page range: 297 - 304

Received: Mar 24, 2021

Accepted: Jun 07, 2021

DOI: https://doi.org/10.33073/pjm-2021-026

Keywords
endophytic bacteria, extracellular enzymes, 16S rRNA gene, Poaceae family, Illumina MiSeq

© 2021 Gokhan Dogan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants

Article Category: original-paper

Published Online: Sep 17, 2021

Page range: 297 - 304

Received: Mar 24, 2021

Accepted: Jun 07, 2021

DOI: https://doi.org/10.33073/pjm-2021-026

Keywordsendophytic bacteria, extracellular enzymes, 16S rRNA gene, Poaceae family, Illumina MiSeq

© 2021 Gokhan Dogan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
endophytic bacteria, extracellular enzymes, 16S rRNA gene, Poaceae family, Illumina MiSeq