<abstract xmlns="http://www.w3.org/1999/xhtml">Bacterial biofilms have recently gained considerable interest in the food production and medical industries due to their ability to resist destruction by disinfectants and other antimicrobials. Biofilms are extracellular polymer matrices that may enhance the survival of pathogens even when exposed to environmental stress. The effect of incubation temperatures (25°C, 37°C, and 40°C) and <italic>Salmonella</italic> serotype on biofilm-forming potentials was evaluated. Previously typed <italic>Salmonella</italic> serotypes (55) isolated from the gut of chickens were accessed for biofilms formation using a standard assay. <italic>Salmonella</italic> Typhimurium ATCC 14028TM and <italic>Salmonella</italic> Enteritidis ATCC 13076TM (positive controls), <italic>Escherichia coli</italic> (internal control) and un-inoculated Luria Bertani (LB) broth (negative control) were used. The isolates formed no biofilm (11.86–13.56%), weak (11.86–45.76%), moderate (18.64–20.34%), strong biofilms (23.73–54.24%) across the various temperatures investigated. Serotypes, <italic>Salmonella</italic> Heidelberg and <italic>Salmonella</italic> Weltevreden were the strongest biofilm formers at temperatures (25°C, 37°C, and 40°C, respectively). The potential of a large proportion (80%) of <italic>Salmonella</italic> serotypes to form biofilms increased with increasing incubation temperatures but decreased at 40°C. Findings indicate that average temperature favours biofilm formation by <italic>Salmonella</italic> serotypes. However, the influence of incubation temperature on biofilm formation was greater when compared to serotype. A positive correlation exists between <italic>Salmonella</italic> biofilm formed at 25°C, 37°C and 40°C (<italic>p</italic> ≥ 0.01). The ability of <italic>Salmonella</italic> species to form biofilms at 25°C and 37°C suggests that these serotypes may present severe challenges to food-processing and hospital facilities.</abstract>

Bacterial biofilms have recently gained considerable interest in the food production and medical industries due to their ability to resist destruction by disinfectants and other antimicrobials. Biofilms are extracellular polymer matrices that may enhance the survival of pathogens even when exposed to environmental stress. The effect of incubation temperatures (25°C, 37°C, and 40°C) and Salmonella serotype on biofilm-forming potentials was evaluated. Previously typed Salmonella serotypes (55) isolated from the gut of chickens were accessed for biofilms formation using a standard assay. Salmonella Typhimurium ATCC 14028TM and Salmonella Enteritidis ATCC 13076TM (positive controls), Escherichia coli (internal control) and un-inoculated Luria Bertani (LB) broth (negative control) were used. The isolates formed no biofilm (11.86–13.56%), weak (11.86–45.76%), moderate (18.64–20.34%), strong biofilms (23.73–54.24%) across the various temperatures investigated. Serotypes, Salmonella Heidelberg and Salmonella Weltevreden were the strongest biofilm formers at temperatures (25°C, 37°C, and 40°C, respectively). The potential of a large proportion (80%) of Salmonella serotypes to form biofilms increased with increasing incubation temperatures but decreased at 40°C. Findings indicate that average temperature favours biofilm formation by Salmonella serotypes. However, the influence of incubation temperature on biofilm formation was greater when compared to serotype. A positive correlation exists between Salmonella biofilm formed at 25°C, 37°C and 40°C (p ≥ 0.01). The ability of Salmonella species to form biofilms at 25°C and 37°C suggests that these serotypes may present severe challenges to food-processing and hospital facilities.

Biofilm Production Potential of <italic>Salmonella</italic> Serovars Isolated from Chickens in North West Province, South Africa

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Biofilm Production Potential of  Serovars Isolated from Chickens in North West Province, South Africa

Bacterial biofilms have recently gained considerable interest in the food production and medical industries due to their ability to resist destruction by...

Biofilm Production Potential of Salmonella Serovars Isolated from Chickens in North West Province, South Africa

Article Category: original-paper

Published Online: Nov 23, 2020

Page range: 427 - 439

Received: Aug 10, 2020

Accepted: Sep 24, 2020

DOI: https://doi.org/10.33073/pjm-2020-046

Keywords
biofilm, biofilm production potential, crystal violet microtitre

© 2020 Stephen Abiola Akinola et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Biofilm Production Potential of Salmonella Serovars Isolated from Chickens in North West Province, South Africa

Article Category: original-paper

Published Online: Nov 23, 2020

Page range: 427 - 439

Received: Aug 10, 2020

Accepted: Sep 24, 2020

DOI: https://doi.org/10.33073/pjm-2020-046

Keywordsbiofilm, biofilm production potential, crystal violet microtitre

© 2020 Stephen Abiola Akinola et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
biofilm, biofilm production potential, crystal violet microtitre