<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Glehnia littoralis</italic> is an endangered medicinal plant growing in the coastal ecological environment and plays an important role in coastal ecosystems. The endophytes in the plant have a significant role in promoting plant growth and enhancing plant stress resistance. However, the endophytic bacterial structure associated with halophyte <italic>G. littoralis</italic> is still not revealed. In this project, the construction and diversity of endophytic bacterial consortium associated with different tissues of <italic>G. littoralis</italic> were illustrated with high throughput sequencing of the V3-V4 region of the bacterial 16S rRNA. The results resolved that the diversity and richness of endophytic bacteria were significantly higher in root than in leaf and stem. The operational taxonomic units (OTU) analysis demonstrated that the Actinobacteria and Proteobacteria were dominant in all the samples at the phylum level, and <italic>Pseudomonas, Bacillus, Rhizobium</italic> were the dominant genera. Our results unraveled that the bacterial communities differed among different tissues of <italic>G. littoralis</italic>. Endophytic bacterial communities in leaf and stem shared more similarity than that in the root. Furthermore, the difference of bacteria community and structure among different tissues were also detected by principal coordinate analysis. Taken altogether, we can conclude that the bacterial communities of different tissues are unique, which could facilitate understanding the diversity of endophytic bacteria in <italic>G. littoralis</italic>.</p></abstract>

Glehnia littoralis is an endangered medicinal plant growing in the coastal ecological environment and plays an important role in coastal ecosystems. The endophytes in the plant have a significant role in promoting plant growth and enhancing plant stress resistance. However, the endophytic bacterial structure associated with halophyte G. littoralis is still not revealed. In this project, the construction and diversity of endophytic bacterial consortium associated with different tissues of G. littoralis were illustrated with high throughput sequencing of the V3-V4 region of the bacterial 16S rRNA. The results resolved that the diversity and richness of endophytic bacteria were significantly higher in root than in leaf and stem. The operational taxonomic units (OTU) analysis demonstrated that the Actinobacteria and Proteobacteria were dominant in all the samples at the phylum level, and Pseudomonas, Bacillus, Rhizobium were the dominant genera. Our results unraveled that the bacterial communities differed among different tissues of G. littoralis. Endophytic bacterial communities in leaf and stem shared more similarity than that in the root. Furthermore, the difference of bacteria community and structure among different tissues were also detected by principal coordinate analysis. Taken altogether, we can conclude that the bacterial communities of different tissues are unique, which could facilitate understanding the diversity of endophytic bacteria in G. littoralis.

Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb <italic>Glehnia littoralis</italic> Based on Illumina Sequencing

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb  Based on Illumina Sequencing

Glehnia littoralis is an endangered medicinal plant growing in the coastal ecological environment and plays an important role in coastal ecosystems. The...

Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Glehnia littoralis Based on Illumina Sequencing

Article Category: original-paper

Published Online: Sep 08, 2020

Page range: 283 - 291

Received: Apr 17, 2020

Accepted: Jun 19, 2020

DOI: https://doi.org/10.33073/pjm-2020-031

Keywords
halophyte, endophytic bacteria, diversity, Illumina sequencing

© 2020 Xiaowei Huo et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Glehnia littoralis Based on Illumina Sequencing

Article Category: original-paper

Published Online: Sep 08, 2020

Page range: 283 - 291

Received: Apr 17, 2020

Accepted: Jun 19, 2020

DOI: https://doi.org/10.33073/pjm-2020-031

Keywordshalophyte, endophytic bacteria, diversity, Illumina sequencing

© 2020 Xiaowei Huo et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
halophyte, endophytic bacteria, diversity, Illumina sequencing