<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study aimed to investigate the PCR-based screening strategy for the prediction of the antimicrobial biosynthetic potential of the selected <italic>Streptomyces</italic> strains originated from an extreme environment (Cholistan Desert, Pakistan). The biosynthetic potential was determined by using both molecular and culture-dependent screening approaches. The four biosynthetic genes clusters, including the <italic>pks-1, nrps</italic>, cyp P450 hydroxylase (<italic>cyps</italic>), and glycopeptide <italic>oxy b</italic> genes, were investigated in the selected strains by PCR amplification, sequencing, and by subsequent bioinformatics approaches. Among the 40 selected <italic>Streptomyces</italic> strains, 33 strains possessed the <italic>nrps</italic> gene, 17 strains carried the <italic>pks-1</italic> gene, four strains were found to have the <italic>cyps</italic> gene, and none of the strain carried <italic>oxy b</italic> gene. The <italic>Streptomyces</italic> strains including NR-1, NR-10, NR-14, and NR-15 were investigated for <italic>in vitro</italic> antifungal activity against <italic>Fusarium oxysporum, Rhizoctonia solani</italic>, and <italic>Aspergillus</italic> sp. The extracts were analyzed for chemical profiling (TLC and HPLC-UV), and a unique pattern of secondary metabolites was observed. The selected strains exhibited pronounced antifungal activity against the fungal test strains with the zone of inhibition up to 17, 18, and 19 mm, respectively. The study depicts that gene-based screening can be successfully applied to identify potentially bioactive strains by usin a single screening process. This PCR-based approach is rapid and can be used for sorting out and selecting the potential candidate among actinobacterial culture collections. Such a preselection or strain prioritization consequently decreases the time and efforts required for selecting the potential bioactive strain, which then can be subjected to the detailed chemical analysis.</p>
</abstract>

This study aimed to investigate the PCR-based screening strategy for the prediction of the antimicrobial biosynthetic potential of the selected Streptomyces strains originated from an extreme environment (Cholistan Desert, Pakistan). The biosynthetic potential was determined by using both molecular and culture-dependent screening approaches. The four biosynthetic genes clusters, including the pks-1, nrps, cyp P450 hydroxylase (cyps), and glycopeptide oxy b genes, were investigated in the selected strains by PCR amplification, sequencing, and by subsequent bioinformatics approaches. Among the 40 selected Streptomyces strains, 33 strains possessed the nrps gene, 17 strains carried the pks-1 gene, four strains were found to have the cyps gene, and none of the strain carried oxy b gene. The Streptomyces strains including NR-1, NR-10, NR-14, and NR-15 were investigated for in vitro antifungal activity against Fusarium oxysporum, Rhizoctonia solani, and Aspergillus sp. The extracts were analyzed for chemical profiling (TLC and HPLC-UV), and a unique pattern of secondary metabolites was observed. The selected strains exhibited pronounced antifungal activity against the fungal test strains with the zone of inhibition up to 17, 18, and 19 mm, respectively. The study depicts that gene-based screening can be successfully applied to identify potentially bioactive strains by usin a single screening process. This PCR-based approach is rapid and can be used for sorting out and selecting the potential candidate among actinobacterial culture collections. Such a preselection or strain prioritization consequently decreases the time and efforts required for selecting the potential bioactive strain, which then can be subjected to the detailed chemical analysis.

PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This study aimed to investigate the PCR-based screening strategy for the prediction of the antimicrobial biosynthetic potential of the selected Streptomyces...

PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains

Article Category: original-paper

Published Online: May 12, 2020

Page range: 139 - 149

Received: Nov 29, 2019

Accepted: Mar 29, 2020

DOI: https://doi.org/10.33073/pjm-2020-016

Keywords
gene-based screening, polyene specific cytochrome P450 hydroxylase (CYP),

© 2020 Naila Noureen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains

Article Category: original-paper

Published Online: May 12, 2020

Page range: 139 - 149

Received: Nov 29, 2019

Accepted: Mar 29, 2020

DOI: https://doi.org/10.33073/pjm-2020-016

Keywordsgene-based screening, polyene specific cytochrome P450 hydroxylase (CYP),

© 2020 Naila Noureen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
gene-based screening, polyene specific cytochrome P450 hydroxylase (CYP),