<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The proteomes of outer membrane vesicles (OMVs) secreted by <italic>C. jejuni</italic> 81–176 strain, which was exposed to oxygen or antibiotic stress (polymyxin B), were characterized. We also assessed the OMVs production and their content in two mutated strains – ∆<italic>dsbI</italic> and ∆<italic>htrA</italic>. OMVs production was significantly increased under the polymyxin B stress and remained unaltered in all other variants. Interestingly, the qualitative load of OMVs was constant regardless of the stress conditions or genetic background. However, certain proteins exhibited notable quantitative changes, ranging from 4-fold decrease to 10-fold increase. Up- or downregulated proteins (e.g. major outer membrane protein <italic>porA</italic>, iron ABC transporter, serine protease- <italic>htrA</italic>, 60 kDa chaperonin-<italic>groL</italic>, enolase) represented various cell compartments (cytoplasm, periplasm, and membrane) and exhibited various functions; nevertheless, one common group was noted that consisted of components of flagellar apparatus, i.e., FlaA/B, FlgC/E, which were mostly upregulated. Some of these proteins are the putative substrates of DsbI protein. Further investigation of the regulation of <italic>C. jejuni</italic> OMVs composition and their role in virulence will allow a better understanding of the infectious process of <italic>C. jejuni</italic>.</p></abstract>

The proteomes of outer membrane vesicles (OMVs) secreted by C. jejuni 81–176 strain, which was exposed to oxygen or antibiotic stress (polymyxin B), were characterized. We also assessed the OMVs production and their content in two mutated strains – ∆dsbI and ∆htrA. OMVs production was significantly increased under the polymyxin B stress and remained unaltered in all other variants. Interestingly, the qualitative load of OMVs was constant regardless of the stress conditions or genetic background. However, certain proteins exhibited notable quantitative changes, ranging from 4-fold decrease to 10-fold increase. Up- or downregulated proteins (e.g. major outer membrane protein porA, iron ABC transporter, serine protease- htrA, 60 kDa chaperonin-groL, enolase) represented various cell compartments (cytoplasm, periplasm, and membrane) and exhibited various functions; nevertheless, one common group was noted that consisted of components of flagellar apparatus, i.e., FlaA/B, FlgC/E, which were mostly upregulated. Some of these proteins are the putative substrates of DsbI protein. Further investigation of the regulation of C. jejuni OMVs composition and their role in virulence will allow a better understanding of the infectious process of C. jejuni.

Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from <italic>Campylobacter jejuni</italic>

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from 

The proteomes of outer membrane vesicles (OMVs) secreted by C. jejuni 81–176 strain, which was exposed to oxygen or antibiotic stress (polymyxin B), were...

Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni

Article Category: original-paper

Published Online: Jun 28, 2019

Page range: 255 - 261

Received: Jan 30, 2019

Accepted: Apr 12, 2019

DOI: https://doi.org/10.33073/pjm-2019-027

Keywords
outer membrane vesicles (OMVs), proteomics

© 2019 Renata Godlewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni

Article Category: original-paper

Published Online: Jun 28, 2019

Page range: 255 - 261

Received: Jan 30, 2019

Accepted: Apr 12, 2019

DOI: https://doi.org/10.33073/pjm-2019-027

Keywordsouter membrane vesicles (OMVs), proteomics

© 2019 Renata Godlewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
outer membrane vesicles (OMVs), proteomics