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Antifouling activity of bacterial extracts associated with soft coral and macroalgae from the Red Sea

Rabab Ibrahim Tunkal
Rabab Ibrahim Tunkal
, 
Mamdoh T. Jamal
Mamdoh T. Jamal
, 
Idris Abdulrahman
Idris Abdulrahman
, 
Arulazhagan Pugazhendi
Arulazhagan Pugazhendi
 and 
Sathianeson Satheesh
Sathianeson Satheesh
   | Jan 18, 2023
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Article Category: Original research paper
Published Online: Jan 18, 2023
Page range: 325 - 336
Received: Oct 10, 2022
Accepted: Nov 17, 2022
DOI: https://doi.org/10.26881/oahs-2022.4.02
Keywords
© 2022 Rabab Ibrahim Tunkal et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Phylogenetic tree showing the two organisms with other related species. The organisms from this study are highlighted in blue, while the red symbol was used to show the bootstrap values.
Phylogenetic tree showing the two organisms with other related species. The organisms from this study are highlighted in blue, while the red symbol was used to show the bootstrap values.

Figure 2

Bacterial growth inhibitory activity of the CSE of MBR1
Bacterial growth inhibitory activity of the CSE of MBR1

Figure 3

Bacterial growth inhibitory activity of the CSE of MBR4
Bacterial growth inhibitory activity of the CSE of MBR4

Figure 4

Percentage of biofilm inhibition activity of the extracts from MBR1 and MBR4 against the biofilm bacteria
Percentage of biofilm inhibition activity of the extracts from MBR1 and MBR4 against the biofilm bacteria

Figure 5

Toxicity of CSE extracted from the bacterial strains against barnacle larvae
Toxicity of CSE extracted from the bacterial strains against barnacle larvae

Figure 6

Antilarval settlement activity of CSE against cyprid-stage larvae of barnacles
Antilarval settlement activity of CSE against cyprid-stage larvae of barnacles

Figure 7

GC-MS spectrum of CSE extracted from the bacterial strain MBR1
GC-MS spectrum of CSE extracted from the bacterial strain MBR1

Figure 8

GC-MS spectrum of CSE extracted from the bacterial strain MBR4
GC-MS spectrum of CSE extracted from the bacterial strain MBR4

Compounds detected in the CSE of MBR1

Compound name RT (min) Peak area
Hexane, 2,2-dimethyl- 2.043 5682084
n-Tridecan-1-ol 7.046 5873147
3-Chloropropionic acid, heptadecyl ester 8.176 2029795
Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester 8.627 13557111
Hexacontane 8.808 5252629
Tetrapentacontane, 1,54-dibromo- 9.027 2603872
Tetrapentacontane, 1,54-dibromo- 9.2 4136789
Tetrapentacontane 9.412 1932874
Tetrapentacontane 9.832 13033013

Compounds detected in the CSE of MBR4

Compound name RT (min) Peak area
Pentane, 2,2,4-trimethyl- 2.04 4716273
Cyclopropane, 1-(1-hydroxy-1-heptyl)-2-methylene-3-pentyl- 8.542 3254671
Octadecanoic acid, 2,3-dihydroxypropyl ester 8.633 5949198
(7R,8R)-cis-anti-cis-Tricyclo[7.3.0.0(2,6)]dodecane-7,8-diol 8.783 6609750
Tetrapentacontane, 1,54-dibromo- 8.945 7398782
7-Hexyltridecan-1-ol 9.258 3273783
9,12,15-Octadecatrienoic acid, 2-phenyl-1,3-dioxan-2-yl ester, (Z,Z,Z)- 9.35 4608587
Tetrapentacontane, 1,54-dibromo- 9.426 4100361
17-Pentatriacontene 9.549 3437915
2H-Pyran-2-one, 5-ethylidenetetrahydro-4-(2-hydroxyethyl)- 9.777 3956877

Post hoc Tukey test results (approximate probabilities) for the antifouling activity of CSE extracted from three bacterial strains against biofilm-forming bacteria and barnacle larvae

Bacterial extract Antibiofilm activity Antilarval selement activity
V. harveyi Planomicrobium sp. Cyprid larvae
Control MBR1 <0.001 <0.01 <0.001
MBR4 <0.001 <0.001 <0.001
MBR1 MBR4 0.067 0.808 0.942
eISSN:
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Language:
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Journal Subjects:
Chemistry, other, Geosciences, Life Sciences
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