<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>In marine environments where biofouling occurs and has an impact on the maritime economy and environment, compounds that inhibit the attachment, growth and survival of microorganisms in a biofilm complex as well as settlement of larvae are considered potential antifouling compounds. In this study, the extracellular metabolites from two surface-associated bacteria isolated from soft coral and macroalga were evaluated for antibiofilm and antisettlement activity. The bacteria were identified using 16S rRNA gene sequencing, and the culture supernatant extract of each strain was evaluated for antibiofilm activity. The compounds present in the extracts were analysed using GC-MS. The two bacterial strains were identified as <italic>Bacillus licheniformis</italic> MBR1 and <italic>Vibrio alginolyticus</italic> MBR4 for the isolates from soft coral and macroalgae, respectively. The extracts inhibited the growth of biofilm-forming bacteria, biofilm formation and barnacle larval settlement. The GC-MS analysis of the extract detected the presence of compounds such as tetrapentacontane, octadecanoic acid, 2,3-dihydroxypropyl ester, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester and 17-pentatriacontene. The results of the study show that extracellular metabolites of the bacteria associated with marine organisms could be used as natural antifouling compounds to control biofouling.</p>
</abstract>

In marine environments where biofouling occurs and has an impact on the maritime economy and environment, compounds that inhibit the attachment, growth and survival of microorganisms in a biofilm complex as well as settlement of larvae are considered potential antifouling compounds. In this study, the extracellular metabolites from two surface-associated bacteria isolated from soft coral and macroalga were evaluated for antibiofilm and antisettlement activity. The bacteria were identified using 16S rRNA gene sequencing, and the culture supernatant extract of each strain was evaluated for antibiofilm activity. The compounds present in the extracts were analysed using GC-MS. The two bacterial strains were identified as Bacillus licheniformis MBR1 and Vibrio alginolyticus MBR4 for the isolates from soft coral and macroalgae, respectively. The extracts inhibited the growth of biofilm-forming bacteria, biofilm formation and barnacle larval settlement. The GC-MS analysis of the extract detected the presence of compounds such as tetrapentacontane, octadecanoic acid, 2,3-dihydroxypropyl ester, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester and 17-pentatriacontene. The results of the study show that extracellular metabolites of the bacteria associated with marine organisms could be used as natural antifouling compounds to control biofouling.

Antifouling activity of bacterial extracts associated with soft coral and macroalgae from the Red Sea

Center of Excellence in Environmental Studies

Oceanological and Hydrobiological Studies

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

In marine environments where biofouling occurs and has an impact on the maritime economy and environment, compounds that inhibit the attachment, growth and...

Antifouling activity of bacterial extracts associated with soft coral and macroalgae from the Red Sea

Article Category: Original research paper

Published Online: Jan 18, 2023

Page range: 325 - 336

Received: Oct 10, 2022

Accepted: Nov 17, 2022

DOI: https://doi.org/10.26881/oahs-2022.4.02

Keywords
antifouling, antibiofilm, antisettlement, marine bacteria, bioactive compounds, Red Sea

© 2022 Rabab Ibrahim Tunkal et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Antifouling activity of bacterial extracts associated with soft coral and macroalgae from the Red Sea

Article Category: Original research paper

Published Online: Jan 18, 2023

Page range: 325 - 336

Received: Oct 10, 2022

Accepted: Nov 17, 2022

DOI: https://doi.org/10.26881/oahs-2022.4.02

Keywordsantifouling, antibiofilm, antisettlement, marine bacteria, bioactive compounds, Red Sea

© 2022 Rabab Ibrahim Tunkal et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
antifouling, antibiofilm, antisettlement, marine bacteria, bioactive compounds, Red Sea