<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Microbiological indicators (total coliforms, faecal coliforms, E. coli and enterococci) and phylogenetic groups (domain Archaea, classes Alpha-, Beta-, Gammaproteobacteria and the Cytophaga-Flavobacterium group) detected by FISH were detected in profiles of a highly anthropogenically-affected stream (Luzická Nisa; Northern Bohemia, Czech Republic). This study aimed to assess the changes in the microbial communities of such a polluted stream, and possible relationships between “classic indicators” and the phylogenetic groups. One particular aim concerned a characterisation of the fluorescence in situ hybridization (FISH) method, the source of any uncertainty and its limit in terms of quality control (QA/QC). Of the phylogenetic groups studied, the Proteobacteria phylum was more abundant in comparison to the Cytophaga-Flavobacterium group or the Archaea domain. The profile Lucany (above the start of city urban areas) was very different from later downstream profiles, because of its very low faecal bacteria content, low counts of Gammaproteobacteria, and evident dominance of the Cytophaga-Flavobacterium group together with Betaproteobacteria. Later profiles did not show such large differences among themselves. The group of Gammaproteobacteria was very common mainly in profiles with high amounts of untreated faecal pollution. The repeatability of counting bacteria by the FISH method was 14 % on average, an “uncertainty” similar to that of cultivation methods.</p></abstract>

Microbiological indicators (total coliforms, faecal coliforms, E. coli and enterococci) and phylogenetic groups (domain Archaea, classes Alpha-, Beta-, Gammaproteobacteria and the Cytophaga-Flavobacterium group) detected by FISH were detected in profiles of a highly anthropogenically-affected stream (Luzická Nisa; Northern Bohemia, Czech Republic). This study aimed to assess the changes in the microbial communities of such a polluted stream, and possible relationships between “classic indicators” and the phylogenetic groups. One particular aim concerned a characterisation of the fluorescence in situ hybridization (FISH) method, the source of any uncertainty and its limit in terms of quality control (QA/QC). Of the phylogenetic groups studied, the Proteobacteria phylum was more abundant in comparison to the Cytophaga-Flavobacterium group or the Archaea domain. The profile Lucany (above the start of city urban areas) was very different from later downstream profiles, because of its very low faecal bacteria content, low counts of Gammaproteobacteria, and evident dominance of the Cytophaga-Flavobacterium group together with Betaproteobacteria. Later profiles did not show such large differences among themselves. The group of Gammaproteobacteria was very common mainly in profiles with high amounts of untreated faecal pollution. The repeatability of counting bacteria by the FISH method was 14 % on average, an “uncertainty” similar to that of cultivation methods.

Microbial communities in a strongly anthropogenic affected stream

T. G. Masaryk Water Research Institute, Public Research Institute, Podbabská 30, 160 00 Prague 6, Czech Republic

Acta Musei Silesiae, Scientiae Naturales

{"article-title":"Microbial communities in a strongly anthropogenic affected stream"}

Microbiological indicators (total coliforms, faecal coliforms, E. coli and enterococci) and phylogenetic groups (domain Archaea, classes Alpha-, Beta-,...

Microbial communities in a strongly anthropogenic affected stream

Published Online: Apr 17, 2013

Page range: 229 - 236

DOI: https://doi.org/10.2478/v10210-012-0023-1

Keywords
Anthropogenically-affected stream, microbiological indicators, FISH, Proteobacteria, Archaea, DAPI

This content is open access.

Microbial communities in a strongly anthropogenic affected stream

Published Online: Apr 17, 2013

Page range: 229 - 236

DOI: https://doi.org/10.2478/v10210-012-0023-1

KeywordsAnthropogenically-affected stream, microbiological indicators, FISH, Proteobacteria, Archaea, DAPI

This content is open access.

Keywords
Anthropogenically-affected stream, microbiological indicators, FISH, Proteobacteria, Archaea, DAPI