1. bookVolume 12 (2012): Issue 6 (December 2012)
Journal Details
License
Format
Journal
eISSN
1335-8871
First Published
07 Mar 2008
Publication timeframe
6 times per year
Languages
English
access type Open Access

Signal Detection of Multi-Channel Capillary Electrophoresis Chip Based on CCD

Published Online: 15 Dec 2012
Volume & Issue: Volume 12 (2012) - Issue 6 (December 2012)
Page range: 272 - 276
Journal Details
License
Format
Journal
eISSN
1335-8871
First Published
07 Mar 2008
Publication timeframe
6 times per year
Languages
English

A kind of multi-channel capillary electrophoresis (CE) chip signal detection system based on CCD was developed. The output signal of the CCD sensor was processed by a series of pre-processing circuits and ADC, and then it was collected by the Field Programmable Gate Array (FPGA) chip which communicated with a host computer. The core in FPGA was designed to control the signal flow of the CCD and transfer the data to PC based on a Nios II embedded soft-processor. The application of PC was used to store the data and demonstrate the curve. The measurement of the fluorescent signals for different concentration Rhodamine B dyes is presented and the comparison with other detection systems is also discussed.

Keywords

[1] Renzi, R.F., Stamps, J., Horn, B.A., Ferko, S., VanderNoot, V.A., West, J.A.A., Crocker, R., Wiedenman, B., Yee, D., Fruetel, J.A. (2005). Handheld microanalytical instrument for chip-based electrophoretic separations of proteins. AnalyticalChemistry, 77 (2), 435-441.Search in Google Scholar

[2] Weiping, Y., Liqun, D., Jing, W., Lingzhi, M., Jianbo, Z. (2005). Simulation and experimental study of PCR chip based on silicon. Sensors and Actuators B:Chemical, 108 (1), 695-699.10.1016/j.snb.2004.11.049Search in Google Scholar

[3] Lo, R.C., Ugaz, V.M. (2008). Microchip DNA electrophoresis with automated whole-gel scanning detection. Lab on a Chip, 8 (12), 2135-2145.10.1039/b811033f19023477Search in Google Scholar

[4] Lv, H.F., Yan, W.P., Pan, L. (2011). Continue flow PCR-CE chip for DNA analysis. Sensor Letters, 9 (2), 755-758.10.1166/sl.2011.1609Search in Google Scholar

[5] Shang, F., Guihen, E., Glennon, J.D. (2012). Recent advances in miniaturisation-The role of microchip electrophoresis in clinical analysis. Electrophoresis, 33 (1), 105-116.10.1002/elps.20110045422124936Search in Google Scholar

[6] Pascali, J.P., Bortolotti, F., Tagliaro, F. (2012). Recent advances in the application of CE to forensic sciences, an update over years 2009-2011. Electrophoresis, 33 (1), 117-126.10.1002/elps.20110046322213526Search in Google Scholar

[7] Mora, M.F., Stockton, A.M., Willis, P.A. (2012). Microchip capillary electrophoresis instrumentation for in situ analysis in the search for extraterrestrial life. Electrophoresis, 33 (17), 2624-2638.10.1002/elps.20120010222965706Search in Google Scholar

[8] Song, J.M., Vo-Dinh, T. (2004). Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay. AnalyticaChimica Acta, 507 (1), 115-121.10.1016/j.aca.2003.11.072Search in Google Scholar

[9] Woolley, A.T., Sensabaugh, G.F., Mathies, R.A. (1997). High-speed DNA genotyping using microfabricated capillary array electrophoresis chips. Analytical Chemistry, 69 (11), 2181-2186.10.1021/ac961237+9183181Search in Google Scholar

[10] Shen, Z., Liu, X., Long, Z., Liu, D., Ye, N., Qin, J., Dai, Z., Lin, B. (2006). Parallel analysis of biomolecules on a microfabricated capillary array chip. Electrophoresis, 27 (5-6), 1084-1092.10.1002/elps.20050068916470779Search in Google Scholar

[11] Hsiung, S.K., Lin, C.H., Lee, G.B. (2005). A microfabricated capillary electrophoresis chip with multiple buried optical fibers and microfocusing lens for multiwavelength detection. Electrophoresis, 26 (6), 1122-1129.10.1002/elps.20041003415704249Search in Google Scholar

[12] Geiger, M., Hogerton, A.L., Bowser, M.T. (2011). Capillary electrophoresis. Analytical Chemistry, 84 (2), 577-596.Search in Google Scholar

[13] Lin, S.W., Chang, C.H., Lin, C.H. (2011). Highthroughput fluorescence detections in microfluidic systems. Genomic Medicine, Biomarkers, and HealthSciences, 3 (1), 27-38.10.1016/S2211-4254(11)60005-8Search in Google Scholar

[14] De Castro, A., Zumel, P., García, O., Riesgo, T., Uceda, J. (2003). Concurrent and simple digital controller of an AC/DC converter with power factor correction based on an FPGA. IEEE Transactions onPower Electronics, 18 (1), 334-343.10.1109/TPEL.2002.807106Search in Google Scholar

[15] Kepa, K., Coburn, D., Dainty, J.C., Morgan, F. (2008). High speed optical wavefront sensing with low cost FPGAs. Measurement Science Review, 8 (4), 87-93.10.2478/v10048-008-0021-zSearch in Google Scholar

[16] Kornaros, G. (2010). A soft multi-core architecture for edge detection and data analysis of microarray images. Journal of Systems Architecture, 56 (1), 48-62.10.1016/j.sysarc.2009.11.004Search in Google Scholar

[17] Zou, L., Fu, Z., Zhao, Y.Z., Yang, J.Y. (2010). A pipelined architecture for real time correction of nonuniformity in infrared focal plane arrays imaging system using multiprocessors. Infrared Physics andTechnology, 53 (4), 254-266.10.1016/j.infrared.2010.03.001Search in Google Scholar

[18] Texas Instruments, Inc. (1997). TLC5510 8-Bit HighspeedAnalog-to-digital Converters.Search in Google Scholar

[19] Kalomiros, J.A., Lygouras, J. (2008). Design and evaluation of a hardware/software FPGA-based system for fast image processing. Microprocessors andMicrosystems, 32 (2), 95-106.10.1016/j.micpro.2007.09.001Search in Google Scholar

[20] Fung, R.F., Weng, M.H., Kung, Y.S. (2009). FPGAbased adaptive backstepping fuzzy control for a micropositioning Scott-Russell mechanism. MechanicalSystems and Signal Processing, 23 (8), 2671-2686.Search in Google Scholar

[21] Kawate, E., Hain, M. (2012). New scatterometer for spatial distribution measurements of light scattering from materials. Measurement Science Review, 12 (2), 56-61.10.2478/v10048-012-0012-ySearch in Google Scholar

[22] Pfleging, W., Kohler, R., Schierjott, P., Hoffmann, W. (2009). Laser patterning and packaging of CCD-CEChips made of PMMA. Sensors and Actuators B:Chemical, 138 (1), 336-343.10.1016/j.snb.2009.01.036Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo