Cross-transferability-based identification and validation of simple sequence repeat (SSR) markers in oaks of western Himalayas
Published Online: Jun 20, 2021
Page range: 108 - 116
DOI: https://doi.org/10.2478/sg-2021-0009
Keywords
© 2021 Chander Shekhar et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Cross-amplification is a cost-effective method to extend the applicability of SSR markers to closely related taxa which lack their own sequence information. In the present study, 35 SSR markers developed in four oak species of Europe, North America and Asia were selected and screened in five species of the western Himalayas. Fifteen markers were successfully amplified in